Anti-angiogenic activity of ShengMaBieJia decoction in vitro and in acute myeloid leukaemia tumour-bearing mouse models
Context ShengMaBieJia decoction (SMBJD) is used to treat solid and hematological tumours; however, its anti-angiogenesis activity remains unclear. Objective This study verified the anti-angiogenic effects of SMBJD in vitro and in tumour-bearing acute myeloid leukaemia (AML) mouse models. Materials a...
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doaj-643b7df44315478cbafef907ce4b2db42021-05-06T15:44:47ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162020-01-0158145446410.1080/13880209.2020.17640591764059Anti-angiogenic activity of ShengMaBieJia decoction in vitro and in acute myeloid leukaemia tumour-bearing mouse modelsMengya Wang0Bangyun Ma1Xingbin Dai2Hong Zhang3Huibo Dai4Jingyu Wang5Li Liu6Xuemei Sun7Department of Hematology, No. 1 Clinical Medical College, Nanjing University of Chinese MedicineDepartment of Hematology, Jiangsu Province Hospital of TCM, The Affiliated Hospital of Nanjing University of TCMDepartment of Hematology, Jiangsu Province Hospital of TCM, The Affiliated Hospital of Nanjing University of TCMDepartment of Hematology, No. 1 Clinical Medical College, Nanjing University of Chinese MedicineDepartment of Hematology, No. 1 Clinical Medical College, Nanjing University of Chinese MedicineDepartment of Hematology, No. 1 Clinical Medical College, Nanjing University of Chinese MedicineThe Central Laboratory of Jiangsu Province Hospital of TCM, The Affiliated Hospital of Nanjing University of TCMDepartment of Hematology, Jiangsu Province Hospital of TCM, The Affiliated Hospital of Nanjing University of TCMContext ShengMaBieJia decoction (SMBJD) is used to treat solid and hematological tumours; however, its anti-angiogenesis activity remains unclear. Objective This study verified the anti-angiogenic effects of SMBJD in vitro and in tumour-bearing acute myeloid leukaemia (AML) mouse models. Materials and methods In vivo, the chicken chorioallantoic membrane (CAM) and BALB/c null mouse xenograft models were treated with SMBJD (0, 2, 4, and 8 mg/mL) for 48 h and for 2 weeks, respectively. Anti-angiogenic activity was assessed according to microvessel density (MVD) and immunohistochemistry (IHC) targeting CD31 and VEGFR2. In vitro, proliferation viability, migratory activity and tube formation were measured. Western blots and polymerase chain reaction (PCR) assays were used to examine the levels of PI3K, Akt, and VEGF. Results HPLC analyses revealed the active constituents of SMBJD such as liquiritin, cimifugin, ferulic, isoferulic, and glycyrrhizic acids. In vitro, SMBJD treatment decreased cellular migration, chemotaxis, and tube formation at non-cytotoxic concentrations (2, 4, and 8 mg/mL) in a time- and dose-dependent manner. The dosage of less than IC20 is considered safe. In vivo, CAM models exhibited a decrease in MVD, and the tissues of xenografted mice possessed reduced CD31 and VEGFR2 expression. Conditioned media (CM) from AML cells (HL60 and NB4 cells) treated with non-cytotoxic doses of SMBJD inhibited chemotactic migration and tube formation in vitro. Both CM (HL60) and CM (NB4) exhibited downregulated expression of PI3K, Akt, and VEGF. Discussion and conclusions SMBJD inhibited angiogenesis in AML through the PI3K/AKT pathway, which might be combined with targeted therapy to provide more effective treatment.http://dx.doi.org/10.1080/13880209.2020.1764059smbjdangiogenesisaml |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mengya Wang Bangyun Ma Xingbin Dai Hong Zhang Huibo Dai Jingyu Wang Li Liu Xuemei Sun |
spellingShingle |
Mengya Wang Bangyun Ma Xingbin Dai Hong Zhang Huibo Dai Jingyu Wang Li Liu Xuemei Sun Anti-angiogenic activity of ShengMaBieJia decoction in vitro and in acute myeloid leukaemia tumour-bearing mouse models Pharmaceutical Biology smbjd angiogenesis aml |
author_facet |
Mengya Wang Bangyun Ma Xingbin Dai Hong Zhang Huibo Dai Jingyu Wang Li Liu Xuemei Sun |
author_sort |
Mengya Wang |
title |
Anti-angiogenic activity of ShengMaBieJia decoction in vitro and in acute myeloid leukaemia tumour-bearing mouse models |
title_short |
Anti-angiogenic activity of ShengMaBieJia decoction in vitro and in acute myeloid leukaemia tumour-bearing mouse models |
title_full |
Anti-angiogenic activity of ShengMaBieJia decoction in vitro and in acute myeloid leukaemia tumour-bearing mouse models |
title_fullStr |
Anti-angiogenic activity of ShengMaBieJia decoction in vitro and in acute myeloid leukaemia tumour-bearing mouse models |
title_full_unstemmed |
Anti-angiogenic activity of ShengMaBieJia decoction in vitro and in acute myeloid leukaemia tumour-bearing mouse models |
title_sort |
anti-angiogenic activity of shengmabiejia decoction in vitro and in acute myeloid leukaemia tumour-bearing mouse models |
publisher |
Taylor & Francis Group |
series |
Pharmaceutical Biology |
issn |
1388-0209 1744-5116 |
publishDate |
2020-01-01 |
description |
Context ShengMaBieJia decoction (SMBJD) is used to treat solid and hematological tumours; however, its anti-angiogenesis activity remains unclear. Objective This study verified the anti-angiogenic effects of SMBJD in vitro and in tumour-bearing acute myeloid leukaemia (AML) mouse models. Materials and methods In vivo, the chicken chorioallantoic membrane (CAM) and BALB/c null mouse xenograft models were treated with SMBJD (0, 2, 4, and 8 mg/mL) for 48 h and for 2 weeks, respectively. Anti-angiogenic activity was assessed according to microvessel density (MVD) and immunohistochemistry (IHC) targeting CD31 and VEGFR2. In vitro, proliferation viability, migratory activity and tube formation were measured. Western blots and polymerase chain reaction (PCR) assays were used to examine the levels of PI3K, Akt, and VEGF. Results HPLC analyses revealed the active constituents of SMBJD such as liquiritin, cimifugin, ferulic, isoferulic, and glycyrrhizic acids. In vitro, SMBJD treatment decreased cellular migration, chemotaxis, and tube formation at non-cytotoxic concentrations (2, 4, and 8 mg/mL) in a time- and dose-dependent manner. The dosage of less than IC20 is considered safe. In vivo, CAM models exhibited a decrease in MVD, and the tissues of xenografted mice possessed reduced CD31 and VEGFR2 expression. Conditioned media (CM) from AML cells (HL60 and NB4 cells) treated with non-cytotoxic doses of SMBJD inhibited chemotactic migration and tube formation in vitro. Both CM (HL60) and CM (NB4) exhibited downregulated expression of PI3K, Akt, and VEGF. Discussion and conclusions SMBJD inhibited angiogenesis in AML through the PI3K/AKT pathway, which might be combined with targeted therapy to provide more effective treatment. |
topic |
smbjd angiogenesis aml |
url |
http://dx.doi.org/10.1080/13880209.2020.1764059 |
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