Bile lipids in rats under chronic social stress

Our experiments studied changes in lipid compound of bile of non-purebred male rats under the condition of social stress while the preparation “Korvitin” was used against the stress. Using the method of thin-layer chromatography, we determined the concentrations of phospholipids, cholesterol and its...

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Main Authors: A. M. Liashevych, I. I. Tubaltseva, Y. M. Reshetnik, O. V. Bondarenko, S. P. Veselsky, M. Y. Makarchuk
Format: Article
Language:English
Published: Oles Honchar Dnipro National University 2017-08-01
Series:Regulatory Mechanisms in Biosystems
Subjects:
Online Access:https://medicine.dp.ua/index.php/med/article/view/350
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spelling doaj-644dcc317c0148f4ae4a4b44b22827982020-11-25T01:12:45Zeng Oles Honchar Dnipro National UniversityRegulatory Mechanisms in Biosystems2519-85212017-08-018335636210.15421/021755312Bile lipids in rats under chronic social stressA. M. Liashevych0I. I. Tubaltseva1Y. M. Reshetnik2O. V. Bondarenko3S. P. Veselsky4M. Y. Makarchuk5Taras Shevchenko National University of KyivTaras Shevchenko National University of KyivTaras Shevchenko National University of KyivTaras Shevchenko National University of KyivTaras Shevchenko National University of KyivTaras Shevchenko National University of KyivOur experiments studied changes in lipid compound of bile of non-purebred male rats under the condition of social stress while the preparation “Korvitin” was used against the stress. Using the method of thin-layer chromatography, we determined the concentrations of phospholipids, cholesterol and its esters, free fatty acids and triglycerides in the animals’ bile, which was obtained through vivesection a day and a month after the rats were first subjected to chronic social stress (model of social defeat), and also in the bile of the animals which were treated intragastrically with “Korvitin” against the stress (1 mg/kg, 7 days). In the bile of the male rats which experienced chronic social stress the concentration of free cholesterol decreased and the content of its esters increased both immediately after the initiation of stress and after a month of exposure to stress. The concentration of free fatty acids in the bile decreased after modeling chronic social stress, but increased in liver secretion, taken a month after the animals had first experienced stress. In the bile of male rats immediately after the procedure of exposing the animals to stress, the content of phospholipids decreased. Using “Korvitin” during the modeling of social stress caused decrease in the content of phospholipids in the rats’ bile and caused significant increase in the concentration of free fatty acids, triglycerides and cholesterol esters in the liver secretion. The study found significant changes in the concentration of lipids in the bile and in their distribution in the organism of male rats under the conditions of experimentally induced chronic stress. The effect of stress on the bile of rats requires further study for determining its pathogenic role.https://medicine.dp.ua/index.php/med/article/view/350liverbile cholesterolbile phospholipidsbile fat acidsbile triglyceridesKorvitinsocial defeat
collection DOAJ
language English
format Article
sources DOAJ
author A. M. Liashevych
I. I. Tubaltseva
Y. M. Reshetnik
O. V. Bondarenko
S. P. Veselsky
M. Y. Makarchuk
spellingShingle A. M. Liashevych
I. I. Tubaltseva
Y. M. Reshetnik
O. V. Bondarenko
S. P. Veselsky
M. Y. Makarchuk
Bile lipids in rats under chronic social stress
Regulatory Mechanisms in Biosystems
liver
bile cholesterol
bile phospholipids
bile fat acids
bile triglycerides
Korvitin
social defeat
author_facet A. M. Liashevych
I. I. Tubaltseva
Y. M. Reshetnik
O. V. Bondarenko
S. P. Veselsky
M. Y. Makarchuk
author_sort A. M. Liashevych
title Bile lipids in rats under chronic social stress
title_short Bile lipids in rats under chronic social stress
title_full Bile lipids in rats under chronic social stress
title_fullStr Bile lipids in rats under chronic social stress
title_full_unstemmed Bile lipids in rats under chronic social stress
title_sort bile lipids in rats under chronic social stress
publisher Oles Honchar Dnipro National University
series Regulatory Mechanisms in Biosystems
issn 2519-8521
publishDate 2017-08-01
description Our experiments studied changes in lipid compound of bile of non-purebred male rats under the condition of social stress while the preparation “Korvitin” was used against the stress. Using the method of thin-layer chromatography, we determined the concentrations of phospholipids, cholesterol and its esters, free fatty acids and triglycerides in the animals’ bile, which was obtained through vivesection a day and a month after the rats were first subjected to chronic social stress (model of social defeat), and also in the bile of the animals which were treated intragastrically with “Korvitin” against the stress (1 mg/kg, 7 days). In the bile of the male rats which experienced chronic social stress the concentration of free cholesterol decreased and the content of its esters increased both immediately after the initiation of stress and after a month of exposure to stress. The concentration of free fatty acids in the bile decreased after modeling chronic social stress, but increased in liver secretion, taken a month after the animals had first experienced stress. In the bile of male rats immediately after the procedure of exposing the animals to stress, the content of phospholipids decreased. Using “Korvitin” during the modeling of social stress caused decrease in the content of phospholipids in the rats’ bile and caused significant increase in the concentration of free fatty acids, triglycerides and cholesterol esters in the liver secretion. The study found significant changes in the concentration of lipids in the bile and in their distribution in the organism of male rats under the conditions of experimentally induced chronic stress. The effect of stress on the bile of rats requires further study for determining its pathogenic role.
topic liver
bile cholesterol
bile phospholipids
bile fat acids
bile triglycerides
Korvitin
social defeat
url https://medicine.dp.ua/index.php/med/article/view/350
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