Neurexin–Neuroligin 1 regulates synaptic morphology and functions via the WAVE regulatory complex in Drosophila neuromuscular junction
Neuroligins are postsynaptic adhesion molecules that are essential for postsynaptic specialization and synaptic function. But the underlying molecular mechanisms of neuroligin functions remain unclear. We found that Drosophila Neuroligin 1 (DNlg1) regulates synaptic structure and function through WA...
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doaj-646643a9036347eabc32dac5f03379ea2021-05-05T15:43:44ZengeLife Sciences Publications LtdeLife2050-084X2018-03-01710.7554/eLife.30457Neurexin–Neuroligin 1 regulates synaptic morphology and functions via the WAVE regulatory complex in Drosophila neuromuscular junctionGuanglin Xing0https://orcid.org/0000-0002-8258-0293Moyi Li1https://orcid.org/0000-0003-3566-3931Yichen Sun2Menglong Rui3Yan Zhuang4Huihui Lv5Junhai Han6https://orcid.org/0000-0001-8941-2578Zhengping Jia7https://orcid.org/0000-0003-4413-5364Wei Xie8https://orcid.org/0000-0002-9179-4787Institute of Life Sciences, the Collaborative Innovation Center for Brain Science, Southeast University, Nanjing, ChinaInstitute of Life Sciences, the Collaborative Innovation Center for Brain Science, Southeast University, Nanjing, China; The Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaInstitute of Life Sciences, the Collaborative Innovation Center for Brain Science, Southeast University, Nanjing, ChinaInstitute of Life Sciences, the Collaborative Innovation Center for Brain Science, Southeast University, Nanjing, ChinaInstitute of Life Sciences, the Collaborative Innovation Center for Brain Science, Southeast University, Nanjing, ChinaThe Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing, ChinaInstitute of Life Sciences, the Collaborative Innovation Center for Brain Science, Southeast University, Nanjing, China; The Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaInstitute of Life Sciences, the Collaborative Innovation Center for Brain Science, Southeast University, Nanjing, China; Neurosciences and Mental Health Program, The Hospital for Sick Children, University of Toronto, Ontario, CanadaInstitute of Life Sciences, the Collaborative Innovation Center for Brain Science, Southeast University, Nanjing, China; The Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaNeuroligins are postsynaptic adhesion molecules that are essential for postsynaptic specialization and synaptic function. But the underlying molecular mechanisms of neuroligin functions remain unclear. We found that Drosophila Neuroligin 1 (DNlg1) regulates synaptic structure and function through WAVE regulatory complex (WRC)-mediated postsynaptic actin reorganization. The disruption of DNlg1, DNlg2, or their presynaptic partner neurexin (DNrx) led to a dramatic decrease in the amount of F-actin. Further study showed that DNlg1, but not DNlg2 or DNlg3, directly interacts with the WRC via its C-terminal interacting receptor sequence. That interaction is required to recruit WRC to the postsynaptic membrane to promote F-actin assembly. Furthermore, the interaction between DNlg1 and the WRC is essential for DNlg1 to rescue the morphological and electrophysiological defects in dnlg1 mutants. Our results reveal a novel mechanism by which the DNrx-DNlg1 trans-synaptic interaction coordinates structural and functional properties at the neuromuscular junction.https://elifesciences.org/articles/30457NeuroliginWAVE regulatory complexpostsynaptic assemblyF-actinsynapse |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guanglin Xing Moyi Li Yichen Sun Menglong Rui Yan Zhuang Huihui Lv Junhai Han Zhengping Jia Wei Xie |
spellingShingle |
Guanglin Xing Moyi Li Yichen Sun Menglong Rui Yan Zhuang Huihui Lv Junhai Han Zhengping Jia Wei Xie Neurexin–Neuroligin 1 regulates synaptic morphology and functions via the WAVE regulatory complex in Drosophila neuromuscular junction eLife Neuroligin WAVE regulatory complex postsynaptic assembly F-actin synapse |
author_facet |
Guanglin Xing Moyi Li Yichen Sun Menglong Rui Yan Zhuang Huihui Lv Junhai Han Zhengping Jia Wei Xie |
author_sort |
Guanglin Xing |
title |
Neurexin–Neuroligin 1 regulates synaptic morphology and functions via the WAVE regulatory complex in Drosophila neuromuscular junction |
title_short |
Neurexin–Neuroligin 1 regulates synaptic morphology and functions via the WAVE regulatory complex in Drosophila neuromuscular junction |
title_full |
Neurexin–Neuroligin 1 regulates synaptic morphology and functions via the WAVE regulatory complex in Drosophila neuromuscular junction |
title_fullStr |
Neurexin–Neuroligin 1 regulates synaptic morphology and functions via the WAVE regulatory complex in Drosophila neuromuscular junction |
title_full_unstemmed |
Neurexin–Neuroligin 1 regulates synaptic morphology and functions via the WAVE regulatory complex in Drosophila neuromuscular junction |
title_sort |
neurexin–neuroligin 1 regulates synaptic morphology and functions via the wave regulatory complex in drosophila neuromuscular junction |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2018-03-01 |
description |
Neuroligins are postsynaptic adhesion molecules that are essential for postsynaptic specialization and synaptic function. But the underlying molecular mechanisms of neuroligin functions remain unclear. We found that Drosophila Neuroligin 1 (DNlg1) regulates synaptic structure and function through WAVE regulatory complex (WRC)-mediated postsynaptic actin reorganization. The disruption of DNlg1, DNlg2, or their presynaptic partner neurexin (DNrx) led to a dramatic decrease in the amount of F-actin. Further study showed that DNlg1, but not DNlg2 or DNlg3, directly interacts with the WRC via its C-terminal interacting receptor sequence. That interaction is required to recruit WRC to the postsynaptic membrane to promote F-actin assembly. Furthermore, the interaction between DNlg1 and the WRC is essential for DNlg1 to rescue the morphological and electrophysiological defects in dnlg1 mutants. Our results reveal a novel mechanism by which the DNrx-DNlg1 trans-synaptic interaction coordinates structural and functional properties at the neuromuscular junction. |
topic |
Neuroligin WAVE regulatory complex postsynaptic assembly F-actin synapse |
url |
https://elifesciences.org/articles/30457 |
work_keys_str_mv |
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