Non-Mulberry and Mulberry Silk Protein Sericins as Potential Media Supplement for Animal Cell Culture
Silk protein sericins, in the recent years, find application in cosmetics and pharmaceuticals and as biomaterials. We investigate the potential of sericin, extracted from both mulberry Bombyx mori and different non-mulberry sources, namely, tropical tasar, Antheraea mylitta; muga, Antheraea assama;...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2016-01-01
|
Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2016/7461041 |
id |
doaj-64669c9fd5ea4420899a3635b17d4d2e |
---|---|
record_format |
Article |
spelling |
doaj-64669c9fd5ea4420899a3635b17d4d2e2020-11-25T01:48:37ZengHindawi LimitedBioMed Research International2314-61332314-61412016-01-01201610.1155/2016/74610417461041Non-Mulberry and Mulberry Silk Protein Sericins as Potential Media Supplement for Animal Cell CultureNeety Sahu0Shilpa Pal1Sunaina Sapru2Joydip Kundu3Sarmistha Talukdar4N. Ibotambi Singh5Juming Yao6Subhas C. Kundu7Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, IndiaDepartment of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, IndiaDepartment of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, IndiaDepartment of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, IndiaDepartment of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, IndiaCentral Muga Eri Research and Training Institute, Lahdoigarh, Jorhat, Assam 785700, IndiaThe Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaDepartment of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, IndiaSilk protein sericins, in the recent years, find application in cosmetics and pharmaceuticals and as biomaterials. We investigate the potential of sericin, extracted from both mulberry Bombyx mori and different non-mulberry sources, namely, tropical tasar, Antheraea mylitta; muga, Antheraea assama; and eri, Samia ricini, as growth supplement in serum-free culture medium. Sericin supplemented media containing different concentrations of sericins from the different species are examined for attachment, growth, proliferation, and morphology of fibrosarcoma cells. The optimum sericin supplementation seems to vary with the source of sericins. The results indicate that all the sericins promote the growth of L929 cells in serum-free culture media; however, S. ricini sericin seems to promote better growth of cells amongst other non-mulberry sericins.http://dx.doi.org/10.1155/2016/7461041 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Neety Sahu Shilpa Pal Sunaina Sapru Joydip Kundu Sarmistha Talukdar N. Ibotambi Singh Juming Yao Subhas C. Kundu |
spellingShingle |
Neety Sahu Shilpa Pal Sunaina Sapru Joydip Kundu Sarmistha Talukdar N. Ibotambi Singh Juming Yao Subhas C. Kundu Non-Mulberry and Mulberry Silk Protein Sericins as Potential Media Supplement for Animal Cell Culture BioMed Research International |
author_facet |
Neety Sahu Shilpa Pal Sunaina Sapru Joydip Kundu Sarmistha Talukdar N. Ibotambi Singh Juming Yao Subhas C. Kundu |
author_sort |
Neety Sahu |
title |
Non-Mulberry and Mulberry Silk Protein Sericins as Potential Media Supplement for Animal Cell Culture |
title_short |
Non-Mulberry and Mulberry Silk Protein Sericins as Potential Media Supplement for Animal Cell Culture |
title_full |
Non-Mulberry and Mulberry Silk Protein Sericins as Potential Media Supplement for Animal Cell Culture |
title_fullStr |
Non-Mulberry and Mulberry Silk Protein Sericins as Potential Media Supplement for Animal Cell Culture |
title_full_unstemmed |
Non-Mulberry and Mulberry Silk Protein Sericins as Potential Media Supplement for Animal Cell Culture |
title_sort |
non-mulberry and mulberry silk protein sericins as potential media supplement for animal cell culture |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2016-01-01 |
description |
Silk protein sericins, in the recent years, find application in cosmetics and pharmaceuticals and as biomaterials. We investigate the potential of sericin, extracted from both mulberry Bombyx mori and different non-mulberry sources, namely, tropical tasar, Antheraea mylitta; muga, Antheraea assama; and eri, Samia ricini, as growth supplement in serum-free culture medium. Sericin supplemented media containing different concentrations of sericins from the different species are examined for attachment, growth, proliferation, and morphology of fibrosarcoma cells. The optimum sericin supplementation seems to vary with the source of sericins. The results indicate that all the sericins promote the growth of L929 cells in serum-free culture media; however, S. ricini sericin seems to promote better growth of cells amongst other non-mulberry sericins. |
url |
http://dx.doi.org/10.1155/2016/7461041 |
work_keys_str_mv |
AT neetysahu nonmulberryandmulberrysilkproteinsericinsaspotentialmediasupplementforanimalcellculture AT shilpapal nonmulberryandmulberrysilkproteinsericinsaspotentialmediasupplementforanimalcellculture AT sunainasapru nonmulberryandmulberrysilkproteinsericinsaspotentialmediasupplementforanimalcellculture AT joydipkundu nonmulberryandmulberrysilkproteinsericinsaspotentialmediasupplementforanimalcellculture AT sarmisthatalukdar nonmulberryandmulberrysilkproteinsericinsaspotentialmediasupplementforanimalcellculture AT nibotambisingh nonmulberryandmulberrysilkproteinsericinsaspotentialmediasupplementforanimalcellculture AT jumingyao nonmulberryandmulberrysilkproteinsericinsaspotentialmediasupplementforanimalcellculture AT subhasckundu nonmulberryandmulberrysilkproteinsericinsaspotentialmediasupplementforanimalcellculture |
_version_ |
1725011145978281984 |