Generation of 20 human induced pluripotent stem cell lines from patients with focal segmental glomerulosclerosis (FSGS)

Generation of 20 human induced pluripotent stem cell lines from patients with focal segmental glomerulosclerosis (FSGS)

Focal segmental glomerulosclerosis (FSGS) is a major cause of familial nephrotic syndrome. We generated 20 induced pluripotent stem cell lines from patients diagnosed with FSGS. The iPSC lines include 8 female and 12 male lines and cover a donor age range from 31 to 78. The lines were generated from...

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Main Authors: Janine Cernoch, Tanja Fisch, Iris Fischer, Kristin Fischer, Anna Iwanska, Norman Krüger, Judit Küchler, Claudia Schaar, Sandra Schommer, Narasimha Telugu, Duncan Miller, Valeria Fernández Vallone, Andreas Kurtz, Petra Reinke, Sebastian Diecke, Harald Stachelscheid
Format: Article
Language:English
Published: Elsevier 2021-07-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S187350612100252X
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spelling doaj-6483970034ec4e99bd13d0f0538933e22021-07-09T04:42:37ZengElsevierStem Cell Research1873-50612021-07-0154102406Generation of 20 human induced pluripotent stem cell lines from patients with focal segmental glomerulosclerosis (FSGS)Janine Cernoch0Tanja Fisch1Iris Fischer2Kristin Fischer3Anna Iwanska4Norman Krüger5Judit Küchler6Claudia Schaar7Sandra Schommer8Narasimha Telugu9Duncan Miller10Valeria Fernández Vallone11Andreas Kurtz12Petra Reinke13Sebastian Diecke14Harald Stachelscheid15Berlin Institute of Health (BIH) at Charité – Universitätsmedizin Berlin, Stem Cell Core Facility, Berlin, GermanyBerlin Institute of Health (BIH) at Charité – Universitätsmedizin Berlin, Stem Cell Core Facility, Berlin, GermanyBerlin Institute of Health (BIH) at Charité – Universitätsmedizin Berlin, Stem Cell Core Facility, Berlin, GermanyBerlin Institute of Health (BIH) at Charité – Universitätsmedizin Berlin, Stem Cell Core Facility, Berlin, GermanyMax Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, GermanyMax Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, GermanyBerlin Institute of Health (BIH) at Charité – Universitätsmedizin Berlin, Stem Cell Core Facility, Berlin, GermanyBerlin Institute of Health (BIH) at Charité – Universitätsmedizin Berlin, Stem Cell Core Facility, Berlin, GermanyMax Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, GermanyMax Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, GermanyMax Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, GermanyBerlin Institute of Health (BIH) at Charité – Universitätsmedizin Berlin, Stem Cell Core Facility, Berlin, GermanyBerlin Institute of Health (BIH) at Charité – Universitätsmedizin Berlin, BIH Center for Regenerative Therapies (BCRT), Berlin, GermanyBerlin Institute of Health (BIH) at Charité – Universitätsmedizin Berlin, BIH Center for Regenerative Therapies (BCRT), Berlin, Germany; Berlin Center for Advanced Therapies (BeCAT), Charité – Universitätsmedizin Berlin, Berlin, Germany; Dept. of Nephrology and Internal Intensive Care, Charité – Universitätsmedizin Berlin, Berlin, GermanyMax Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany; Corresponding authors.Berlin Institute of Health (BIH) at Charité – Universitätsmedizin Berlin, Stem Cell Core Facility, Berlin, Germany; Berlin Institute of Health (BIH) at Charité – Universitätsmedizin Berlin, BIH Center for Regenerative Therapies (BCRT), Berlin, Germany; Corresponding authors.Focal segmental glomerulosclerosis (FSGS) is a major cause of familial nephrotic syndrome. We generated 20 induced pluripotent stem cell lines from patients diagnosed with FSGS. The iPSC lines include 8 female and 12 male lines and cover a donor age range from 31 to 78. The lines were generated from peripheral blood mononuclear cells by integration-free reprogramming using Sendai virus vectors. Cell lines were fully characterized regarding their pluripotency and differentiation potential, and quality controlled for karyotypic integrity, identity and clearance of reprogramming vectors. The generated cell lines represent a valuable tool for disease modelling and drug development for FSGS.http://www.sciencedirect.com/science/article/pii/S187350612100252X
collection DOAJ
language English
format Article
sources DOAJ
author Janine Cernoch
Tanja Fisch
Iris Fischer
Kristin Fischer
Anna Iwanska
Norman Krüger
Judit Küchler
Claudia Schaar
Sandra Schommer
Narasimha Telugu
Duncan Miller
Valeria Fernández Vallone
Andreas Kurtz
Petra Reinke
Sebastian Diecke
Harald Stachelscheid
spellingShingle Janine Cernoch
Tanja Fisch
Iris Fischer
Kristin Fischer
Anna Iwanska
Norman Krüger
Judit Küchler
Claudia Schaar
Sandra Schommer
Narasimha Telugu
Duncan Miller
Valeria Fernández Vallone
Andreas Kurtz
Petra Reinke
Sebastian Diecke
Harald Stachelscheid
Generation of 20 human induced pluripotent stem cell lines from patients with focal segmental glomerulosclerosis (FSGS)
Stem Cell Research
author_facet Janine Cernoch
Tanja Fisch
Iris Fischer
Kristin Fischer
Anna Iwanska
Norman Krüger
Judit Küchler
Claudia Schaar
Sandra Schommer
Narasimha Telugu
Duncan Miller
Valeria Fernández Vallone
Andreas Kurtz
Petra Reinke
Sebastian Diecke
Harald Stachelscheid
author_sort Janine Cernoch
title Generation of 20 human induced pluripotent stem cell lines from patients with focal segmental glomerulosclerosis (FSGS)
title_short Generation of 20 human induced pluripotent stem cell lines from patients with focal segmental glomerulosclerosis (FSGS)
title_full Generation of 20 human induced pluripotent stem cell lines from patients with focal segmental glomerulosclerosis (FSGS)
title_fullStr Generation of 20 human induced pluripotent stem cell lines from patients with focal segmental glomerulosclerosis (FSGS)
title_full_unstemmed Generation of 20 human induced pluripotent stem cell lines from patients with focal segmental glomerulosclerosis (FSGS)
title_sort generation of 20 human induced pluripotent stem cell lines from patients with focal segmental glomerulosclerosis (fsgs)
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2021-07-01
description Focal segmental glomerulosclerosis (FSGS) is a major cause of familial nephrotic syndrome. We generated 20 induced pluripotent stem cell lines from patients diagnosed with FSGS. The iPSC lines include 8 female and 12 male lines and cover a donor age range from 31 to 78. The lines were generated from peripheral blood mononuclear cells by integration-free reprogramming using Sendai virus vectors. Cell lines were fully characterized regarding their pluripotency and differentiation potential, and quality controlled for karyotypic integrity, identity and clearance of reprogramming vectors. The generated cell lines represent a valuable tool for disease modelling and drug development for FSGS.
url http://www.sciencedirect.com/science/article/pii/S187350612100252X
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