The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts
Platelet lysate (PL) provides a natural source of growth factors and other bioactive molecules, and the local controlled release of these bioactive PL components is capable of improving the healing of chronic wounds. Therefore, we prepared composite nanofibrous meshes via the needleless electrospinn...
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doaj-64aaa119ffbc430a94aa1a1c2938f0912021-04-13T23:03:57ZengMDPI AGNanomaterials2079-49912021-04-011199599510.3390/nano11040995The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and FibroblastsElena Filova0Andreu Blanquer1Jarmila Knitlova2Martin Plencner3Vera Jencova4Barbora Koprivova5Maxim Lisnenko6Eva Kuzelova Kostakova7Renata Prochazkova8Lucie Bacakova9Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, 1083, 142 20 Videnska, Prague, Czech RepublicDepartment of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, 1083, 142 20 Videnska, Prague, Czech RepublicDepartment of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, 1083, 142 20 Videnska, Prague, Czech RepublicDepartment of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, 1083, 142 20 Videnska, Prague, Czech RepublicTechnical University of Liberec, Faculty of Science, Humanities and Education, Department of Chemistry, Studentska 1402/2, 461 17 Liberec, Czech RepublicTechnical University of Liberec, Faculty of Science, Humanities and Education, Department of Chemistry, Studentska 1402/2, 461 17 Liberec, Czech RepublicTechnical University of Liberec, Faculty of Science, Humanities and Education, Department of Chemistry, Studentska 1402/2, 461 17 Liberec, Czech RepublicTechnical University of Liberec, Faculty of Science, Humanities and Education, Department of Chemistry, Studentska 1402/2, 461 17 Liberec, Czech RepublicRegional Hospital Liberec, Husova 357/10, 460 63 Liberec, Czech RepublicDepartment of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, 1083, 142 20 Videnska, Prague, Czech RepublicPlatelet lysate (PL) provides a natural source of growth factors and other bioactive molecules, and the local controlled release of these bioactive PL components is capable of improving the healing of chronic wounds. Therefore, we prepared composite nanofibrous meshes via the needleless electrospinning technique using poly(vinyl alcohol) (PVA) with a high molecular weight and with a high degree of hydrolysis with the incorporated PL (10% <i>w/w</i>). The morphology, wettability and protein release from the nanofibers was then assessed from the resulting composite PVA–PL nanomats. The bioactivity of the PVA–PL nanomats was proved in vitro using HaCaT keratinocytes, human saphenous endothelial cells (HSVECs) and 3T3 fibroblasts. The PVA–PL supported cell adhesion, proliferation, and viability. The improved phenotypic maturation of the HaCaT cells due to the PVA–PL was manifested via the formation of intermediate filaments positive for cytokeratin 10. The PVA–PL enhanced both the synthesis of the von Willebrand factor via HSVECs and HSVECs chemotaxis through membranes with 8 µm-sized pores. These results indicated the favorable effects of the PVA–PL nanomats on the three cell types involved in the wound healing process, and established PVA–PL nanomats as a promising candidate for further evaluation with respect to in vivo experiments.https://www.mdpi.com/2079-4991/11/4/995controlled releaseplatelet lysatePVA nanofibersendothelial cellskeratinocytesfibroblasts |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elena Filova Andreu Blanquer Jarmila Knitlova Martin Plencner Vera Jencova Barbora Koprivova Maxim Lisnenko Eva Kuzelova Kostakova Renata Prochazkova Lucie Bacakova |
spellingShingle |
Elena Filova Andreu Blanquer Jarmila Knitlova Martin Plencner Vera Jencova Barbora Koprivova Maxim Lisnenko Eva Kuzelova Kostakova Renata Prochazkova Lucie Bacakova The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts Nanomaterials controlled release platelet lysate PVA nanofibers endothelial cells keratinocytes fibroblasts |
author_facet |
Elena Filova Andreu Blanquer Jarmila Knitlova Martin Plencner Vera Jencova Barbora Koprivova Maxim Lisnenko Eva Kuzelova Kostakova Renata Prochazkova Lucie Bacakova |
author_sort |
Elena Filova |
title |
The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts |
title_short |
The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts |
title_full |
The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts |
title_fullStr |
The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts |
title_full_unstemmed |
The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts |
title_sort |
effect of the controlled release of platelet lysate from pva nanomats on keratinocytes, endothelial cells and fibroblasts |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2021-04-01 |
description |
Platelet lysate (PL) provides a natural source of growth factors and other bioactive molecules, and the local controlled release of these bioactive PL components is capable of improving the healing of chronic wounds. Therefore, we prepared composite nanofibrous meshes via the needleless electrospinning technique using poly(vinyl alcohol) (PVA) with a high molecular weight and with a high degree of hydrolysis with the incorporated PL (10% <i>w/w</i>). The morphology, wettability and protein release from the nanofibers was then assessed from the resulting composite PVA–PL nanomats. The bioactivity of the PVA–PL nanomats was proved in vitro using HaCaT keratinocytes, human saphenous endothelial cells (HSVECs) and 3T3 fibroblasts. The PVA–PL supported cell adhesion, proliferation, and viability. The improved phenotypic maturation of the HaCaT cells due to the PVA–PL was manifested via the formation of intermediate filaments positive for cytokeratin 10. The PVA–PL enhanced both the synthesis of the von Willebrand factor via HSVECs and HSVECs chemotaxis through membranes with 8 µm-sized pores. These results indicated the favorable effects of the PVA–PL nanomats on the three cell types involved in the wound healing process, and established PVA–PL nanomats as a promising candidate for further evaluation with respect to in vivo experiments. |
topic |
controlled release platelet lysate PVA nanofibers endothelial cells keratinocytes fibroblasts |
url |
https://www.mdpi.com/2079-4991/11/4/995 |
work_keys_str_mv |
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