Correlation Between Protein Primary Structure and Soluble Expression Level of HSA dAb in Escherichia coli

Correlation Between Protein Primary Structure and Soluble Expression Level of HSA dAb in Escherichia coli

It is widely accepted that features such as pI, length, molecular mass and amino acid (AA) sequence have a significant influence on protein solubility. Here, we mainly focused on AA composition and explored those that most affected the soluble expression level of human serum albumin (HSA) domain ant...

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Bibliographic Details
Main Authors: Yankun Yang, Guoqiang Liu, Meng Liu, Zhonghu Bai, Xiuxia Liu, Wenwen Guo, Xiaofeng Dai
Format: Article
Language:English
Published: University of Zagreb 2018-01-01
Series:Food Technology and Biotechnology
Subjects:
domain antibody (dAb)
Escherichia coli
heterologous protein soluble expression
linear modelling
primary structure
Online Access:http://hrcak.srce.hr/file/290750

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