Nonrandom DNA Segregation Detection under Replication Stress

Summary: Nonrandom DNA segregation (NDS) is a mitotic event in which sister chromatids carrying the old (parent) DNA strands are distributed exclusively to one of the two daughter cells. Although this phenomenon occurs in multiple organisms, the low frequency poses an obstacle to observation. Here,...

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Main Authors:	Sisi Chen, Fang Ji, Meichun Xing, Gaoying Chen, Jiaqian Li, Xinwei Geng, Chao Zhang, Madiha Zahra Syeda, Songmin Ying
Format:	Article
Language:	English
Published:	Elsevier 2020-12-01
Series:	STAR Protocols
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166720301301

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record_format	Article
spelling	doaj-64be0bd3de7942d59c39def67535a72e2020-12-21T04:48:51ZengElsevierSTAR Protocols2666-16672020-12-0113100143Nonrandom DNA Segregation Detection under Replication StressSisi Chen0Fang Ji1Meichun Xing2Gaoying Chen3Jiaqian Li4Xinwei Geng5Chao Zhang6Madiha Zahra Syeda7Songmin Ying8Department of Pharmacology and Department of Respiratory and Critical Care Medicine of the Second Affiliated Hospital, Zhejiang University School of Medicine, Key Laboratory of Respiratory Disease of Zhejiang Province, Hangzhou, Zhejiang 310009, ChinaDepartment of Pharmacology and Department of Respiratory and Critical Care Medicine of the Second Affiliated Hospital, Zhejiang University School of Medicine, Key Laboratory of Respiratory Disease of Zhejiang Province, Hangzhou, Zhejiang 310009, ChinaDepartment of Pathology, Shanghai Tenth People’s Hospital Affiliated to Tongji University, Shanghai 200072, ChinaDepartment of Pharmacology and Department of Respiratory and Critical Care Medicine of the Second Affiliated Hospital, Zhejiang University School of Medicine, Key Laboratory of Respiratory Disease of Zhejiang Province, Hangzhou, Zhejiang 310009, ChinaDepartment of Pharmacology and Department of Respiratory and Critical Care Medicine of the Second Affiliated Hospital, Zhejiang University School of Medicine, Key Laboratory of Respiratory Disease of Zhejiang Province, Hangzhou, Zhejiang 310009, ChinaDepartment of Pharmacology and Department of Respiratory and Critical Care Medicine of the Second Affiliated Hospital, Zhejiang University School of Medicine, Key Laboratory of Respiratory Disease of Zhejiang Province, Hangzhou, Zhejiang 310009, ChinaDepartment of Pharmacology and Department of Respiratory and Critical Care Medicine of the Second Affiliated Hospital, Zhejiang University School of Medicine, Key Laboratory of Respiratory Disease of Zhejiang Province, Hangzhou, Zhejiang 310009, ChinaDepartment of Pharmacology and Department of Respiratory and Critical Care Medicine of the Second Affiliated Hospital, Zhejiang University School of Medicine, Key Laboratory of Respiratory Disease of Zhejiang Province, Hangzhou, Zhejiang 310009, ChinaDepartment of Pharmacology and Department of Respiratory and Critical Care Medicine of the Second Affiliated Hospital, Zhejiang University School of Medicine, Key Laboratory of Respiratory Disease of Zhejiang Province, Hangzhou, Zhejiang 310009, China; International Institutes of Medicine, the Fourth Affiliated Hospital of Zhejiang University School of Medicine, Yiwu 322000, China; Corresponding authorSummary: Nonrandom DNA segregation (NDS) is a mitotic event in which sister chromatids carrying the old (parent) DNA strands are distributed exclusively to one of the two daughter cells. Although this phenomenon occurs in multiple organisms, the low frequency poses an obstacle to observation. Here, we present an improved protocol to induce NDS under replication stress. This protocol can be modified to accommodate various cell lines.For complete details on the use and execution of this protocol, please refer to Xing et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720301301
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Sisi Chen Fang Ji Meichun Xing Gaoying Chen Jiaqian Li Xinwei Geng Chao Zhang Madiha Zahra Syeda Songmin Ying
spellingShingle	Sisi Chen Fang Ji Meichun Xing Gaoying Chen Jiaqian Li Xinwei Geng Chao Zhang Madiha Zahra Syeda Songmin Ying Nonrandom DNA Segregation Detection under Replication Stress STAR Protocols
author_facet	Sisi Chen Fang Ji Meichun Xing Gaoying Chen Jiaqian Li Xinwei Geng Chao Zhang Madiha Zahra Syeda Songmin Ying
author_sort	Sisi Chen
title	Nonrandom DNA Segregation Detection under Replication Stress
title_short	Nonrandom DNA Segregation Detection under Replication Stress
title_full	Nonrandom DNA Segregation Detection under Replication Stress
title_fullStr	Nonrandom DNA Segregation Detection under Replication Stress
title_full_unstemmed	Nonrandom DNA Segregation Detection under Replication Stress
title_sort	nonrandom dna segregation detection under replication stress
publisher	Elsevier
series	STAR Protocols
issn	2666-1667
publishDate	2020-12-01
description	Summary: Nonrandom DNA segregation (NDS) is a mitotic event in which sister chromatids carrying the old (parent) DNA strands are distributed exclusively to one of the two daughter cells. Although this phenomenon occurs in multiple organisms, the low frequency poses an obstacle to observation. Here, we present an improved protocol to induce NDS under replication stress. This protocol can be modified to accommodate various cell lines.For complete details on the use and execution of this protocol, please refer to Xing et al. (2020).
url	http://www.sciencedirect.com/science/article/pii/S2666166720301301
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Nonrandom DNA Segregation Detection under Replication Stress

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