Genomic DNA from rat blood: A comparison of two extraction methods

• Main Author: Takić Miladinov, D.
• Format: Article
• Language: English
• Published: University of Niš 2016-09-01
• Series: Biologica Nyssana
• Subjects: DNA extraction; CTAB; salting out; rat whole blood; DNA extraction; CTAB; salting out; rat whole blood
• Online Access: http://journal.pmf.ni.ac.rs/bionys/index.php/bionys/article/view/144/105

In this study, two methods for DNA extraction from fresh rat blood were compared. One is based on the use of cetyltrimethylammonium bromide (CTAB method), while the other one is well-known salting out method. Spectrophotometric analysis was employed to assess yield and purity of isolated DNA, while agarose gel electrophoresis was carried out to evaluate DNA integrity. The results have clearly demonstrated that the extraction method has significantly influenced the quantity and purity of isolated DNA. By using the CTAB method, a larger quantity of high-molecular weight DNA with good purity is obtained which, along with timeand cost-efficiency of the procedure, makes this method more suitable for the extraction of DNA from rat whole blood.