Anti-fatigue activity of purified flavonoids prepared from chestnut (Castanea mollissima) flower
Castanea mollissima Blume has been used to treat a variety of diseases in Chinese traditional medicine. This study was aimed to examine the anti-fatigue effect of purified Chestnut flower flavonoids (CFF) in mice and its underlying mechanisms using the weight-loaded swimming test. The study revealed...
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doaj-64c338c8e9a648079aeb938de131966e2021-04-30T07:20:31ZengElsevierJournal of Functional Foods1756-46462021-04-0179104365Anti-fatigue activity of purified flavonoids prepared from chestnut (Castanea mollissima) flowerFei Peng0Hongyang Yin1Bin Du2Kui Niu3Xin Ren4Yuedong Yang5Hebei Key Laboratory of Active Components and Functions in Natural Products (under Planning), Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; Collaborative Innovation Centre of Hebei Chestnut Industry, Hebei Normal University of Science and Technology, Qinhuangdao 066000, ChinaHebei Key Laboratory of Active Components and Functions in Natural Products (under Planning), Hebei Normal University of Science and Technology, Qinhuangdao 066000, ChinaHebei Key Laboratory of Active Components and Functions in Natural Products (under Planning), Hebei Normal University of Science and Technology, Qinhuangdao 066000, ChinaHebei Key Laboratory of Active Components and Functions in Natural Products (under Planning), Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; Collaborative Innovation Centre of Hebei Chestnut Industry, Hebei Normal University of Science and Technology, Qinhuangdao 066000, ChinaHebei Key Laboratory of Active Components and Functions in Natural Products (under Planning), Hebei Normal University of Science and Technology, Qinhuangdao 066000, ChinaHebei Key Laboratory of Active Components and Functions in Natural Products (under Planning), Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; Collaborative Innovation Centre of Hebei Chestnut Industry, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; Corresponding author at: Hebei Normal University of Science and Technology, No. 360 Hebei Road, Haigang District, Qinhuangdao 066000, China.Castanea mollissima Blume has been used to treat a variety of diseases in Chinese traditional medicine. This study was aimed to examine the anti-fatigue effect of purified Chestnut flower flavonoids (CFF) in mice and its underlying mechanisms using the weight-loaded swimming test. The study revealed that the content of total flavonoids in CFF was 754.20 mgRE/gdm, which identified six main types of flavonoids, myricetin-3-O-glucoside, quercetin-3-O-rutinoside, quercetin-O-glucoside, kaempherol-3-O-rutinoside, isorhamnetin-3-O-rutinoside, kaempferol, respectively. CFF showed excellent free radical scavenging and anti-fatigue effect. Notably, CFF significantly prolonged the swimming time of mice, while down-regulating the levels of lactate dehydrogenase (LDH), blood lactic acid (BLA), blood urea nitrogen (BUN), and creatine kinase (CK), and increasing liver and muscle glycogen. Meanwhile, CFF suppressed exercise-elicited oxidative stress through promoting the SOD activity and reducing the MDA level. Furthermore, CFF up-regulated PGC-1α and PPARα expression in mouse skeletal muscle. In conclusion, these findings indicate that CFF exerts an anti-fatigue effect presumably via energy reserves and oxidative stress inhibition.http://www.sciencedirect.com/science/article/pii/S1756464621000141Chestnut flowerFlavonoidsAnti-fatigueOxidative stress |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fei Peng Hongyang Yin Bin Du Kui Niu Xin Ren Yuedong Yang |
spellingShingle |
Fei Peng Hongyang Yin Bin Du Kui Niu Xin Ren Yuedong Yang Anti-fatigue activity of purified flavonoids prepared from chestnut (Castanea mollissima) flower Journal of Functional Foods Chestnut flower Flavonoids Anti-fatigue Oxidative stress |
author_facet |
Fei Peng Hongyang Yin Bin Du Kui Niu Xin Ren Yuedong Yang |
author_sort |
Fei Peng |
title |
Anti-fatigue activity of purified flavonoids prepared from chestnut (Castanea mollissima) flower |
title_short |
Anti-fatigue activity of purified flavonoids prepared from chestnut (Castanea mollissima) flower |
title_full |
Anti-fatigue activity of purified flavonoids prepared from chestnut (Castanea mollissima) flower |
title_fullStr |
Anti-fatigue activity of purified flavonoids prepared from chestnut (Castanea mollissima) flower |
title_full_unstemmed |
Anti-fatigue activity of purified flavonoids prepared from chestnut (Castanea mollissima) flower |
title_sort |
anti-fatigue activity of purified flavonoids prepared from chestnut (castanea mollissima) flower |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2021-04-01 |
description |
Castanea mollissima Blume has been used to treat a variety of diseases in Chinese traditional medicine. This study was aimed to examine the anti-fatigue effect of purified Chestnut flower flavonoids (CFF) in mice and its underlying mechanisms using the weight-loaded swimming test. The study revealed that the content of total flavonoids in CFF was 754.20 mgRE/gdm, which identified six main types of flavonoids, myricetin-3-O-glucoside, quercetin-3-O-rutinoside, quercetin-O-glucoside, kaempherol-3-O-rutinoside, isorhamnetin-3-O-rutinoside, kaempferol, respectively. CFF showed excellent free radical scavenging and anti-fatigue effect. Notably, CFF significantly prolonged the swimming time of mice, while down-regulating the levels of lactate dehydrogenase (LDH), blood lactic acid (BLA), blood urea nitrogen (BUN), and creatine kinase (CK), and increasing liver and muscle glycogen. Meanwhile, CFF suppressed exercise-elicited oxidative stress through promoting the SOD activity and reducing the MDA level. Furthermore, CFF up-regulated PGC-1α and PPARα expression in mouse skeletal muscle. In conclusion, these findings indicate that CFF exerts an anti-fatigue effect presumably via energy reserves and oxidative stress inhibition. |
topic |
Chestnut flower Flavonoids Anti-fatigue Oxidative stress |
url |
http://www.sciencedirect.com/science/article/pii/S1756464621000141 |
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