The HMGA1 Pseudogene 7 Induces miR-483 and miR-675 Upregulation by Activating Egr1 through a ceRNA Mechanism
Several studies have established that pseudogene mRNAs can work as competing endogenous RNAs and, when deregulated, play a key role in the onset of human neoplasias. Recently, we have isolated two HMGA1 pseudogenes, HMGA1P6 and HMGA1P7. These pseudogenes have a critical role in cancer progression, a...
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doaj-64d3f94016f045679beab8bb5f7cff9c2020-11-25T00:53:00ZengMDPI AGGenes2073-44252017-11-0181133010.3390/genes8110330genes8110330The HMGA1 Pseudogene 7 Induces miR-483 and miR-675 Upregulation by Activating Egr1 through a ceRNA MechanismMarco De Martino0Giuseppe Palma1Amalia Azzariti2Claudio Arra3Alfredo Fusco4Francesco Esposito5Istituto di Endocrinologia ed Oncologia Sperimentale del CNR c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli “Federico II”, via Pansini, 5, 80131 Naples, ItalyIstituto Nazionale dei Tumori, Fondazione Pascale, via Mariano Semmola, 52, 80131 Naples, ItalyIRCCS Istituto Tumori Giovanni Paolo II, Viale O. Flacco, 65, 70124 Bari, ItalyIstituto Nazionale dei Tumori, Fondazione Pascale, via Mariano Semmola, 52, 80131 Naples, ItalyIstituto di Endocrinologia ed Oncologia Sperimentale del CNR c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli “Federico II”, via Pansini, 5, 80131 Naples, ItalyIstituto di Endocrinologia ed Oncologia Sperimentale del CNR c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli “Federico II”, via Pansini, 5, 80131 Naples, ItalySeveral studies have established that pseudogene mRNAs can work as competing endogenous RNAs and, when deregulated, play a key role in the onset of human neoplasias. Recently, we have isolated two HMGA1 pseudogenes, HMGA1P6 and HMGA1P7. These pseudogenes have a critical role in cancer progression, acting as micro RNA (miRNA) sponges for HMGA1 and other cancer-related genes. HMGA1 pseudogenes were found overexpressed in several human carcinomas, and their expression levels positively correlate with an advanced cancer stage and a poor prognosis. In order to investigate the molecular alterations following HMGA1 pseudogene 7 overexpression, we carried out miRNA sequencing analysis on HMGA1P7 overexpressing mouse embryonic fibroblasts. Intriguingly, the most upregulated miRNAs were miR-483 and miR-675 that have been described as key regulators in cancer progression. Here, we report that HMGA1P7 upregulates miR-483 and miR-675 through a competing endogenous RNA mechanism with Egr1, a transcriptional factor that positively regulates miR-483 and miR-675 expression.https://www.mdpi.com/2073-4425/8/11/330HMGA1 pseudogenesHMGA1P7micro RNAceRNA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marco De Martino Giuseppe Palma Amalia Azzariti Claudio Arra Alfredo Fusco Francesco Esposito |
spellingShingle |
Marco De Martino Giuseppe Palma Amalia Azzariti Claudio Arra Alfredo Fusco Francesco Esposito The HMGA1 Pseudogene 7 Induces miR-483 and miR-675 Upregulation by Activating Egr1 through a ceRNA Mechanism Genes HMGA1 pseudogenes HMGA1P7 micro RNA ceRNA |
author_facet |
Marco De Martino Giuseppe Palma Amalia Azzariti Claudio Arra Alfredo Fusco Francesco Esposito |
author_sort |
Marco De Martino |
title |
The HMGA1 Pseudogene 7 Induces miR-483 and miR-675 Upregulation by Activating Egr1 through a ceRNA Mechanism |
title_short |
The HMGA1 Pseudogene 7 Induces miR-483 and miR-675 Upregulation by Activating Egr1 through a ceRNA Mechanism |
title_full |
The HMGA1 Pseudogene 7 Induces miR-483 and miR-675 Upregulation by Activating Egr1 through a ceRNA Mechanism |
title_fullStr |
The HMGA1 Pseudogene 7 Induces miR-483 and miR-675 Upregulation by Activating Egr1 through a ceRNA Mechanism |
title_full_unstemmed |
The HMGA1 Pseudogene 7 Induces miR-483 and miR-675 Upregulation by Activating Egr1 through a ceRNA Mechanism |
title_sort |
hmga1 pseudogene 7 induces mir-483 and mir-675 upregulation by activating egr1 through a cerna mechanism |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2017-11-01 |
description |
Several studies have established that pseudogene mRNAs can work as competing endogenous RNAs and, when deregulated, play a key role in the onset of human neoplasias. Recently, we have isolated two HMGA1 pseudogenes, HMGA1P6 and HMGA1P7. These pseudogenes have a critical role in cancer progression, acting as micro RNA (miRNA) sponges for HMGA1 and other cancer-related genes. HMGA1 pseudogenes were found overexpressed in several human carcinomas, and their expression levels positively correlate with an advanced cancer stage and a poor prognosis. In order to investigate the molecular alterations following HMGA1 pseudogene 7 overexpression, we carried out miRNA sequencing analysis on HMGA1P7 overexpressing mouse embryonic fibroblasts. Intriguingly, the most upregulated miRNAs were miR-483 and miR-675 that have been described as key regulators in cancer progression. Here, we report that HMGA1P7 upregulates miR-483 and miR-675 through a competing endogenous RNA mechanism with Egr1, a transcriptional factor that positively regulates miR-483 and miR-675 expression. |
topic |
HMGA1 pseudogenes HMGA1P7 micro RNA ceRNA |
url |
https://www.mdpi.com/2073-4425/8/11/330 |
work_keys_str_mv |
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