Comparative Evaluation of RNAlater Solution and Snap Frozen Methods for Gene Expression Studies in Different Tissues

Comparative Evaluation of RNAlater Solution and Snap Frozen Methods for Gene Expression Studies in Different Tissues

Introduction: Freezing of tissues with liquid nitrogen is the most common method in studies performed at the RNA level. However, the use of RNA stabilization solutions has become a popular alternative method. The aim of this study is to investigate the effectiveness of RNAlater on RNA stabilization...

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Bibliographic Details
Main Authors: Ozkan Huseyin, Kerman Enes
Format: Article
Language:English
Published: Sciendo 2020-07-01
Series:Romanian Journal of Laboratory Medicine
Subjects:
rnalater
snap frozen
rna quality
reference gene
gene expression
Online Access:https://doi.org/10.2478/rrlm-2020-0024
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spelling doaj-64e9b979d0884d0a887c6857cecdfeb82021-09-05T14:01:31ZengSciendoRomanian Journal of Laboratory Medicine2284-56232020-07-0128328729710.2478/rrlm-2020-0024rrlm-2020-0024Comparative Evaluation of RNAlater Solution and Snap Frozen Methods for Gene Expression Studies in Different TissuesOzkan Huseyin0Kerman Enes1Hatay Mustafa Kemal University, Faculty of Veterinary Medicine, Dept. of Genetic, Hatay, TurkeyDept. of Forensic Clinic, Aydın Public Hospital, Aydın, TurkeyIntroduction: Freezing of tissues with liquid nitrogen is the most common method in studies performed at the RNA level. However, the use of RNA stabilization solutions has become a popular alternative method. The aim of this study is to investigate the effectiveness of RNAlater on RNA stabilization in different tissues.https://doi.org/10.2478/rrlm-2020-0024rnalatersnap frozenrna qualityreference genegene expression
collection DOAJ
language English
format Article
sources DOAJ
author Ozkan Huseyin
Kerman Enes
spellingShingle Ozkan Huseyin
Kerman Enes
Comparative Evaluation of RNAlater Solution and Snap Frozen Methods for Gene Expression Studies in Different Tissues
Romanian Journal of Laboratory Medicine
rnalater
snap frozen
rna quality
reference gene
gene expression
author_facet Ozkan Huseyin
Kerman Enes
author_sort Ozkan Huseyin
title Comparative Evaluation of RNAlater Solution and Snap Frozen Methods for Gene Expression Studies in Different Tissues
title_short Comparative Evaluation of RNAlater Solution and Snap Frozen Methods for Gene Expression Studies in Different Tissues
title_full Comparative Evaluation of RNAlater Solution and Snap Frozen Methods for Gene Expression Studies in Different Tissues
title_fullStr Comparative Evaluation of RNAlater Solution and Snap Frozen Methods for Gene Expression Studies in Different Tissues
title_full_unstemmed Comparative Evaluation of RNAlater Solution and Snap Frozen Methods for Gene Expression Studies in Different Tissues
title_sort comparative evaluation of rnalater solution and snap frozen methods for gene expression studies in different tissues
publisher Sciendo
series Romanian Journal of Laboratory Medicine
issn 2284-5623
publishDate 2020-07-01
description Introduction: Freezing of tissues with liquid nitrogen is the most common method in studies performed at the RNA level. However, the use of RNA stabilization solutions has become a popular alternative method. The aim of this study is to investigate the effectiveness of RNAlater on RNA stabilization in different tissues.
topic rnalater
snap frozen
rna quality
reference gene
gene expression
url https://doi.org/10.2478/rrlm-2020-0024
work_keys_str_mv AT ozkanhuseyin comparativeevaluationofrnalatersolutionandsnapfrozenmethodsforgeneexpressionstudiesindifferenttissues
AT kermanenes comparativeevaluationofrnalatersolutionandsnapfrozenmethodsforgeneexpressionstudiesindifferenttissues
_version_ 1717810050940010496

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