Production of Functional Buttermilk and Soymilk Using <i>Pediococcus acidilactici</i> BD16 (<i>alaD</i>⁺)

• Main Authors: Anshula Sharma, Masafumi Noda, Masanori Sugiyama, Ajaz Ahmad, Baljinder Kaur
• Format: Article
• Language: English
• Published: MDPI AG 2021-08-01
• Series: Molecules
• Subjects: bioactive metabolites; functional drinks; L-alanine; LAB fermentation; metabolomic fingerprinting; <i>Pediococcus acidilactici</i>
• Online Access: https://www.mdpi.com/1420-3049/26/15/4671

Functional foods or drinks prepared using lactic acid bacteria (LAB) have recently gained considerable attention because they can offer additional nutritional and health benefits. The present study aimed to develop functional drinks by the fermentation of buttermilk and soymilk preparations using the <i>Pediococcus acidilactici</i> BD16 (<i>alaD</i>⁺) strain expressing the L-alanine dehydrogenase enzyme. LAB fermentation was carried out for 24 h and its impact on the physicochemical and quality attributes of the fermented drinks was evaluated. Levels of total antioxidants, phenolics, flavonoids, and especially L-alanine enhanced significantly after LAB fermentation. Further, GC-MS-based metabolomic fingerprinting was performed to identify the presence of bioactive metabolites such as 1,2-benzenedicarboxylic acid, 1-dodecene, 2-aminononadecane, 3-octadecene, 4-octen-3-one, acetic acid, azanonane, benzaldehyde, benzoic acid, chloroacetic acid, colchicine, heptadecanenitrile, hexadecanal, quercetin, and triacontane, which could be accountable for the improvement of organoleptic attributes and health benefits of the drinks. Meanwhile, the levels of certain undesirable metabolites such as 1-pentadecene, 2-bromopropionic acid, 8-heptadecene, formic acid, and propionic acid, which impart bitterness, rancidity, and unpleasant odor to the fermented drinks, were reduced considerably after LAB fermentation. This study is probably the first of its kind that highlights the application of <i>P. acidilactici</i> BD16 (<i>alaD</i>⁺) as a starter culture candidate for the production of functional buttermilk and soymilk.