Protective effects of ciliary neurotrophic factor on the retinal ganglion cells by injure of hydrogen peroxide
AIM: To explore the effect of ciliary neurotrophic factor (CNTF) on retinal ganglion cell (RGC)-5 induced by hydrogen peroxide (H2O2). METHODS: After cell adherence, RGC-5 culture medium was changed to contain different concentrations of H2O2 from 50 to 150 µmol/L at four time points (0.5, 1, 1.5 a...
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doaj-6501c5406e064c61966fc08887930ee92020-11-24T21:41:03ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982018-06-0111692392810.18240/ijo.2018.06.05Protective effects of ciliary neurotrophic factor on the retinal ganglion cells by injure of hydrogen peroxideWen-Jun Wang0Wei Jin1An-Huai Yang2Zhen Chen3Yi-Qiao Xing4Eye Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, ChinaEye Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, ChinaEye Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, ChinaEye Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, ChinaEye Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, ChinaAIM: To explore the effect of ciliary neurotrophic factor (CNTF) on retinal ganglion cell (RGC)-5 induced by hydrogen peroxide (H2O2). METHODS: After cell adherence, RGC-5 culture medium was changed to contain different concentrations of H2O2 from 50 to 150 µmol/L at four time points (0.5, 1, 1.5 and 2h) to select the concentration and time point for H2O2 induced model. Two different ways of interventions for injured RGC-5 cells respectively were CNTF as an addition in the culture medium or recombinant lentiviral plasmid carrying CNTF gene transfecting bone mesenchymal stem cells (BMSCs) for co-culture with RGC-5. RESULTS: Compared to the control group, H2O2 led to RGC-5 death closely associated with concentrations and action time of H2O2 and we chose 125 µmol/L and 2h to establish the H2O2-induced model. While CNTF inhibited the loss of RGC-5 cells obviously with a dose-dependent survival rate. Nevertheless two administration routes had different survival rate yet higher rate in recombinant lentiviral plasmid group but there were no statistically significant differences. CONCLUSION: Both the two administration routes of CNTF have effects on RGC-5 cells induced by H2O2. If their own advantages were combined, there may be a better administration route.http://www.ijo.cn/en_publish/2018/6/20180605.pdf928retinal ganglion cellsciliary neurotrophic factorhydrogen peroxideneuroprotectionrecombinant lentiviral vector |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wen-Jun Wang Wei Jin An-Huai Yang Zhen Chen Yi-Qiao Xing |
spellingShingle |
Wen-Jun Wang Wei Jin An-Huai Yang Zhen Chen Yi-Qiao Xing Protective effects of ciliary neurotrophic factor on the retinal ganglion cells by injure of hydrogen peroxide International Journal of Ophthalmology 928 retinal ganglion cells ciliary neurotrophic factor hydrogen peroxide neuroprotection recombinant lentiviral vector |
author_facet |
Wen-Jun Wang Wei Jin An-Huai Yang Zhen Chen Yi-Qiao Xing |
author_sort |
Wen-Jun Wang |
title |
Protective effects of ciliary neurotrophic factor on the retinal ganglion cells by injure of hydrogen peroxide |
title_short |
Protective effects of ciliary neurotrophic factor on the retinal ganglion cells by injure of hydrogen peroxide |
title_full |
Protective effects of ciliary neurotrophic factor on the retinal ganglion cells by injure of hydrogen peroxide |
title_fullStr |
Protective effects of ciliary neurotrophic factor on the retinal ganglion cells by injure of hydrogen peroxide |
title_full_unstemmed |
Protective effects of ciliary neurotrophic factor on the retinal ganglion cells by injure of hydrogen peroxide |
title_sort |
protective effects of ciliary neurotrophic factor on the retinal ganglion cells by injure of hydrogen peroxide |
publisher |
Press of International Journal of Ophthalmology (IJO PRESS) |
series |
International Journal of Ophthalmology |
issn |
2222-3959 2227-4898 |
publishDate |
2018-06-01 |
description |
AIM: To explore the effect of ciliary neurotrophic factor (CNTF) on retinal ganglion cell (RGC)-5 induced by hydrogen peroxide (H2O2).
METHODS: After cell adherence, RGC-5 culture medium was changed to contain different concentrations of H2O2 from 50 to 150 µmol/L at four time points (0.5, 1, 1.5 and 2h) to select the concentration and time point for H2O2 induced model. Two different ways of interventions for injured RGC-5 cells respectively were CNTF as an addition in the culture medium or recombinant lentiviral plasmid carrying CNTF gene transfecting bone mesenchymal stem cells (BMSCs) for co-culture with RGC-5.
RESULTS: Compared to the control group, H2O2 led to RGC-5 death closely associated with concentrations and action time of H2O2 and we chose 125 µmol/L and 2h to establish the H2O2-induced model. While CNTF inhibited the loss of RGC-5 cells obviously with a dose-dependent survival rate. Nevertheless two administration routes had different survival rate yet higher rate in recombinant lentiviral plasmid group but there were no statistically significant differences.
CONCLUSION: Both the two administration routes of CNTF have effects on RGC-5 cells induced by H2O2. If their own advantages were combined, there may be a better administration route. |
topic |
928 retinal ganglion cells ciliary neurotrophic factor hydrogen peroxide neuroprotection recombinant lentiviral vector |
url |
http://www.ijo.cn/en_publish/2018/6/20180605.pdf |
work_keys_str_mv |
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1725923472020865024 |