A Fungal Diterpene Synthase Is Responsible for Sterol Biosynthesis for Growth
A conserved open reading frame, dps, is described in Pestalotiopsis microspora, sharing a remarkable similarity with fungal diterpene synthases whose function is less studied. Loss-of-function approach manifested that dps was necessary for the growth and the development of the fungus. A deletion str...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2020-07-01
|
Series: | Frontiers in Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.01426/full |
id |
doaj-650c3e1d0a97471bb4882e1263a55662 |
---|---|
record_format |
Article |
spelling |
doaj-650c3e1d0a97471bb4882e1263a556622020-11-25T03:38:46ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-07-011110.3389/fmicb.2020.01426537340A Fungal Diterpene Synthase Is Responsible for Sterol Biosynthesis for GrowthYanjie Liu0Anqing Duan1Longfei Chen2Longfei Chen3Dan Wang4Qiaohong Xie5Biyun Xiang6Yamin Lin7Xiaoran Hao8Xudong Zhu9Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing, ChinaBeijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing, ChinaZhejiang Medicine Co., Ltd., Zhejiang, ChinaDepartment of Microbiology, College of Life Sciences, Nankai University, Tianjin, ChinaDepartment of Microbiology, College of Life Sciences, Nankai University, Tianjin, ChinaBeijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing, ChinaBeijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing, ChinaBeijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing, ChinaBeijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing, ChinaBeijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing, ChinaA conserved open reading frame, dps, is described in Pestalotiopsis microspora, sharing a remarkable similarity with fungal diterpene synthases whose function is less studied. Loss-of-function approach manifested that dps was necessary for the growth and the development of the fungus. A deletion strain, dpsΔ, showed a fundamental retardation in growth, which could deliberately be restored by the addition of exogenous sterols to the media. Gas chromatography–mass spectrometry analysis confirmed the loss of the ability to produce certain sterols. Thus, the tolerance and the resistance of dpsΔ to several stress conditions were impaired. Secondary metabolites, such as the polyketide derivative dibenzodioxocinones, were significantly diminished. At the molecular level, the deletion of dps even affected the expression of genes in the mevalonate pathway. This report adds knowledge about fungal diterpene synthases in Pestalitiopsis microspora.https://www.frontiersin.org/article/10.3389/fmicb.2020.01426/fullPestalotiopsis microsporaditerpene synthaseMVA genessterol biosynthesispolyketide |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yanjie Liu Anqing Duan Longfei Chen Longfei Chen Dan Wang Qiaohong Xie Biyun Xiang Yamin Lin Xiaoran Hao Xudong Zhu |
spellingShingle |
Yanjie Liu Anqing Duan Longfei Chen Longfei Chen Dan Wang Qiaohong Xie Biyun Xiang Yamin Lin Xiaoran Hao Xudong Zhu A Fungal Diterpene Synthase Is Responsible for Sterol Biosynthesis for Growth Frontiers in Microbiology Pestalotiopsis microspora diterpene synthase MVA genes sterol biosynthesis polyketide |
author_facet |
Yanjie Liu Anqing Duan Longfei Chen Longfei Chen Dan Wang Qiaohong Xie Biyun Xiang Yamin Lin Xiaoran Hao Xudong Zhu |
author_sort |
Yanjie Liu |
title |
A Fungal Diterpene Synthase Is Responsible for Sterol Biosynthesis for Growth |
title_short |
A Fungal Diterpene Synthase Is Responsible for Sterol Biosynthesis for Growth |
title_full |
A Fungal Diterpene Synthase Is Responsible for Sterol Biosynthesis for Growth |
title_fullStr |
A Fungal Diterpene Synthase Is Responsible for Sterol Biosynthesis for Growth |
title_full_unstemmed |
A Fungal Diterpene Synthase Is Responsible for Sterol Biosynthesis for Growth |
title_sort |
fungal diterpene synthase is responsible for sterol biosynthesis for growth |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2020-07-01 |
description |
A conserved open reading frame, dps, is described in Pestalotiopsis microspora, sharing a remarkable similarity with fungal diterpene synthases whose function is less studied. Loss-of-function approach manifested that dps was necessary for the growth and the development of the fungus. A deletion strain, dpsΔ, showed a fundamental retardation in growth, which could deliberately be restored by the addition of exogenous sterols to the media. Gas chromatography–mass spectrometry analysis confirmed the loss of the ability to produce certain sterols. Thus, the tolerance and the resistance of dpsΔ to several stress conditions were impaired. Secondary metabolites, such as the polyketide derivative dibenzodioxocinones, were significantly diminished. At the molecular level, the deletion of dps even affected the expression of genes in the mevalonate pathway. This report adds knowledge about fungal diterpene synthases in Pestalitiopsis microspora. |
topic |
Pestalotiopsis microspora diterpene synthase MVA genes sterol biosynthesis polyketide |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2020.01426/full |
work_keys_str_mv |
AT yanjieliu afungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT anqingduan afungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT longfeichen afungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT longfeichen afungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT danwang afungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT qiaohongxie afungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT biyunxiang afungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT yaminlin afungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT xiaoranhao afungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT xudongzhu afungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT yanjieliu fungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT anqingduan fungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT longfeichen fungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT longfeichen fungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT danwang fungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT qiaohongxie fungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT biyunxiang fungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT yaminlin fungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT xiaoranhao fungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth AT xudongzhu fungalditerpenesynthaseisresponsibleforsterolbiosynthesisforgrowth |
_version_ |
1724540619473289216 |