Activity of encapsulated Lactobacillus bulgaricus in alginate-whey protein microspheres
In this work, alginate-whey protein was used as wall materials for encapsulating Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus). The characteristics of encapsulated and free L. bulgaricus showed that the free L. bulgaricus lost viability after 1 min exposure to simulated gastric fluid (...
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Instituto de Tecnologia do Paraná (Tecpar)
2014-10-01
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doaj-650f00c598ac4d59975619c33ea9ec492020-11-24T21:57:51ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242014-10-0157573674110.1590/S1516-8913201402377S1516-89132014000500736Activity of encapsulated Lactobacillus bulgaricus in alginate-whey protein microspheresMeng-Yan ChenWei ZhengQiu-Yue DongZhen-Hua LiLu-E ShiZhen-Xing TangIn this work, alginate-whey protein was used as wall materials for encapsulating Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus). The characteristics of encapsulated and free L. bulgaricus showed that the free L. bulgaricus lost viability after 1 min exposure to simulated gastric fluid (SGF) at pH 2.0 and 2.5. However, the viability of encapsulated L. bulgaricus did not decrease in SGF at pH 2.5 for 2 h incubation. The viable numbers of encapsulated L. bulgaricus decreased less than 1.0 log unit for 2 h incubation in SGF at pH 2.0. For bile stability, only 1.2 log units and 2.0 log units viability of the encapsulated L. bulgaricus was lost in 1 and 2% bile for 1 h exposure, respectively, compared with no survival of free L. bulgaricus under the same conditions. Encapsulated L. bulgaricus was completely released from the microspheres in simulated intestinal fluid (SIF, pH 6.8) in 3 h. The viability of the encapsulated L. bulgaricus retained more 8.0 log CFU/g after stored at 4°C for four weeks. However, for free L. bulgaricus, only around 3.0 log CFU/mL was found at the same storage conditions. Results showed that the encapsulation could improve the stability of L. bulgaricus.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500736&lng=en&tlng=enEncapsulationAlginateWhey proteinProbioticsStability |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Meng-Yan Chen Wei Zheng Qiu-Yue Dong Zhen-Hua Li Lu-E Shi Zhen-Xing Tang |
spellingShingle |
Meng-Yan Chen Wei Zheng Qiu-Yue Dong Zhen-Hua Li Lu-E Shi Zhen-Xing Tang Activity of encapsulated Lactobacillus bulgaricus in alginate-whey protein microspheres Brazilian Archives of Biology and Technology Encapsulation Alginate Whey protein Probiotics Stability |
author_facet |
Meng-Yan Chen Wei Zheng Qiu-Yue Dong Zhen-Hua Li Lu-E Shi Zhen-Xing Tang |
author_sort |
Meng-Yan Chen |
title |
Activity of encapsulated Lactobacillus bulgaricus in alginate-whey protein microspheres |
title_short |
Activity of encapsulated Lactobacillus bulgaricus in alginate-whey protein microspheres |
title_full |
Activity of encapsulated Lactobacillus bulgaricus in alginate-whey protein microspheres |
title_fullStr |
Activity of encapsulated Lactobacillus bulgaricus in alginate-whey protein microspheres |
title_full_unstemmed |
Activity of encapsulated Lactobacillus bulgaricus in alginate-whey protein microspheres |
title_sort |
activity of encapsulated lactobacillus bulgaricus in alginate-whey protein microspheres |
publisher |
Instituto de Tecnologia do Paraná (Tecpar) |
series |
Brazilian Archives of Biology and Technology |
issn |
1678-4324 |
publishDate |
2014-10-01 |
description |
In this work, alginate-whey protein was used as wall materials for encapsulating Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus). The characteristics of encapsulated and free L. bulgaricus showed that the free L. bulgaricus lost viability after 1 min exposure to simulated gastric fluid (SGF) at pH 2.0 and 2.5. However, the viability of encapsulated L. bulgaricus did not decrease in SGF at pH 2.5 for 2 h incubation. The viable numbers of encapsulated L. bulgaricus decreased less than 1.0 log unit for 2 h incubation in SGF at pH 2.0. For bile stability, only 1.2 log units and 2.0 log units viability of the encapsulated L. bulgaricus was lost in 1 and 2% bile for 1 h exposure, respectively, compared with no survival of free L. bulgaricus under the same conditions. Encapsulated L. bulgaricus was completely released from the microspheres in simulated intestinal fluid (SIF, pH 6.8) in 3 h. The viability of the encapsulated L. bulgaricus retained more 8.0 log CFU/g after stored at 4°C for four weeks. However, for free L. bulgaricus, only around 3.0 log CFU/mL was found at the same storage conditions. Results showed that the encapsulation could improve the stability of L. bulgaricus. |
topic |
Encapsulation Alginate Whey protein Probiotics Stability |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500736&lng=en&tlng=en |
work_keys_str_mv |
AT mengyanchen activityofencapsulatedlactobacillusbulgaricusinalginatewheyproteinmicrospheres AT weizheng activityofencapsulatedlactobacillusbulgaricusinalginatewheyproteinmicrospheres AT qiuyuedong activityofencapsulatedlactobacillusbulgaricusinalginatewheyproteinmicrospheres AT zhenhuali activityofencapsulatedlactobacillusbulgaricusinalginatewheyproteinmicrospheres AT lueshi activityofencapsulatedlactobacillusbulgaricusinalginatewheyproteinmicrospheres AT zhenxingtang activityofencapsulatedlactobacillusbulgaricusinalginatewheyproteinmicrospheres |
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