Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells
In the present paper, we developed a primary culture of Rhodnius prolixus salivary gland and main salivary canal cells. Cells remained viable in culture for 30 days. Three types of cells were indentified in the salivary gland cultures, with binuclear cells being the most abundant. The supernatants o...
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Instituto Oswaldo Cruz, Ministério da Saúde
2010-03-01
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doaj-651071b786924ad888ad46dafaa51a092020-11-25T01:09:44ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.0074-02761678-80602010-03-01105213213610.1590/S0074-02762010000200004Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cellsFernanda F RochaRicardo N AraujoLuciana M SilvaNelder F GontijoMarcos H PereiraIn the present paper, we developed a primary culture of Rhodnius prolixus salivary gland and main salivary canal cells. Cells remained viable in culture for 30 days. Three types of cells were indentified in the salivary gland cultures, with binuclear cells being the most abundant. The supernatants of salivary cultures contained mainly 16-24 kDa proteins and presented anticoagulant and apyrase activities. Secretion vesicles were observed budding from the cellular monolayer of the main salivary canal cells. These results indicate that R. prolixus salivary proteins may be produced in vitro and suggest that the main salivary canal may have a possible secretory role.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000200004Rhodnius prolixussalivary glandprimary culture |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fernanda F Rocha Ricardo N Araujo Luciana M Silva Nelder F Gontijo Marcos H Pereira |
spellingShingle |
Fernanda F Rocha Ricardo N Araujo Luciana M Silva Nelder F Gontijo Marcos H Pereira Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells Memórias do Instituto Oswaldo Cruz. Rhodnius prolixus salivary gland primary culture |
author_facet |
Fernanda F Rocha Ricardo N Araujo Luciana M Silva Nelder F Gontijo Marcos H Pereira |
author_sort |
Fernanda F Rocha |
title |
Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells |
title_short |
Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells |
title_full |
Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells |
title_fullStr |
Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells |
title_full_unstemmed |
Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells |
title_sort |
primary culture of rhodnius prolixus (hemiptera: reduviidae) salivary gland cells |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
0074-0276 1678-8060 |
publishDate |
2010-03-01 |
description |
In the present paper, we developed a primary culture of Rhodnius prolixus salivary gland and main salivary canal cells. Cells remained viable in culture for 30 days. Three types of cells were indentified in the salivary gland cultures, with binuclear cells being the most abundant. The supernatants of salivary cultures contained mainly 16-24 kDa proteins and presented anticoagulant and apyrase activities. Secretion vesicles were observed budding from the cellular monolayer of the main salivary canal cells. These results indicate that R. prolixus salivary proteins may be produced in vitro and suggest that the main salivary canal may have a possible secretory role. |
topic |
Rhodnius prolixus salivary gland primary culture |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000200004 |
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