Microdissection testicular sperm extraction in five Japanese patients with non‐mosaic Klinefelter's syndrome
Abstract Cases Microdissection testicular sperm extraction (micro‐TESE) was performed on five Japanese men with non‐mosaic Klinefelter's syndrome (KS) and non‐obstructive azoospermia in the authors' department. Here is reported the operative results and partner's clinical course for t...
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doaj-652ea64457b74d6789bae32638b084cd2020-11-24T21:26:02ZengWileyReproductive Medicine and Biology1445-57811447-05782018-04-0117220921610.1002/rmb2.12092Microdissection testicular sperm extraction in five Japanese patients with non‐mosaic Klinefelter's syndromeMakoto Chihara0Kanna Ogi1Tatsuya Ishiguro2Kunihiko Yoshida3Chikako Godo4Koichi Takakuwa5Takayuki Enomoto6Department of Obstetrics and Gynecology Niigata University Medical and Dental Hospital Niigata JapanDepartment of Obstetrics and Gynecology Niigata University Medical and Dental Hospital Niigata JapanDepartment of Obstetrics and Gynecology Niigata University Medical and Dental Hospital Niigata JapanDepartment of Obstetrics and Gynecology Niigata University Medical and Dental Hospital Niigata JapanDepartment of Obstetrics and Gynecology Niigata University Medical and Dental Hospital Niigata JapanDepartment of Obstetrics and Gynecology Niigata University Medical and Dental Hospital Niigata JapanDepartment of Obstetrics and Gynecology Niigata University Medical and Dental Hospital Niigata JapanAbstract Cases Microdissection testicular sperm extraction (micro‐TESE) was performed on five Japanese men with non‐mosaic Klinefelter's syndrome (KS) and non‐obstructive azoospermia in the authors' department. Here is reported the operative results and partner's clinical course for two cases where spermatozoa could be acquired. Also encountered was a man with non‐mosaic KS with the partial deletion of azoospermia factor (AZF)b. Because this is rare, it is reported in detail in the context of the previous literature. This case series describes the first experience of micro‐TESE by gynecologists in the current department. Outcome The egg collection date was adjusted to the micro‐TESE day by using the modified ultra‐long method. Intracytoplasmic sperm injection (ICSI) was implemented for two men whose spermatozoa were acquired by micro‐TESE, with these progressing to the blastocyst stage. Subsequently, one case conceived after the transfer of fresh embryos and a healthy baby was delivered. However, spermatozoa could not be retrieved from the man with non‐mosaic KS who was harboring the partial deletion of AZFb. Conclusion These findings suggest that ovulation induction by using the modified ultra‐long method with micro‐TESE and ICSI on the same day represents an effective treatment option for men with non‐mosaic KS. As there are cases where AZF deletion is recognized among patients with non‐mosaic KS, screening before micro‐TESE is strongly recommended.https://doi.org/10.1002/rmb2.12092azoospermiainfertilityintracytoplasmic sperm injectionKlinefelter's syndromepregnancy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Makoto Chihara Kanna Ogi Tatsuya Ishiguro Kunihiko Yoshida Chikako Godo Koichi Takakuwa Takayuki Enomoto |
spellingShingle |
Makoto Chihara Kanna Ogi Tatsuya Ishiguro Kunihiko Yoshida Chikako Godo Koichi Takakuwa Takayuki Enomoto Microdissection testicular sperm extraction in five Japanese patients with non‐mosaic Klinefelter's syndrome Reproductive Medicine and Biology azoospermia infertility intracytoplasmic sperm injection Klinefelter's syndrome pregnancy |
author_facet |
Makoto Chihara Kanna Ogi Tatsuya Ishiguro Kunihiko Yoshida Chikako Godo Koichi Takakuwa Takayuki Enomoto |
author_sort |
Makoto Chihara |
title |
Microdissection testicular sperm extraction in five Japanese patients with non‐mosaic Klinefelter's syndrome |
title_short |
Microdissection testicular sperm extraction in five Japanese patients with non‐mosaic Klinefelter's syndrome |
title_full |
Microdissection testicular sperm extraction in five Japanese patients with non‐mosaic Klinefelter's syndrome |
title_fullStr |
Microdissection testicular sperm extraction in five Japanese patients with non‐mosaic Klinefelter's syndrome |
title_full_unstemmed |
Microdissection testicular sperm extraction in five Japanese patients with non‐mosaic Klinefelter's syndrome |
title_sort |
microdissection testicular sperm extraction in five japanese patients with non‐mosaic klinefelter's syndrome |
publisher |
Wiley |
series |
Reproductive Medicine and Biology |
issn |
1445-5781 1447-0578 |
publishDate |
2018-04-01 |
description |
Abstract Cases Microdissection testicular sperm extraction (micro‐TESE) was performed on five Japanese men with non‐mosaic Klinefelter's syndrome (KS) and non‐obstructive azoospermia in the authors' department. Here is reported the operative results and partner's clinical course for two cases where spermatozoa could be acquired. Also encountered was a man with non‐mosaic KS with the partial deletion of azoospermia factor (AZF)b. Because this is rare, it is reported in detail in the context of the previous literature. This case series describes the first experience of micro‐TESE by gynecologists in the current department. Outcome The egg collection date was adjusted to the micro‐TESE day by using the modified ultra‐long method. Intracytoplasmic sperm injection (ICSI) was implemented for two men whose spermatozoa were acquired by micro‐TESE, with these progressing to the blastocyst stage. Subsequently, one case conceived after the transfer of fresh embryos and a healthy baby was delivered. However, spermatozoa could not be retrieved from the man with non‐mosaic KS who was harboring the partial deletion of AZFb. Conclusion These findings suggest that ovulation induction by using the modified ultra‐long method with micro‐TESE and ICSI on the same day represents an effective treatment option for men with non‐mosaic KS. As there are cases where AZF deletion is recognized among patients with non‐mosaic KS, screening before micro‐TESE is strongly recommended. |
topic |
azoospermia infertility intracytoplasmic sperm injection Klinefelter's syndrome pregnancy |
url |
https://doi.org/10.1002/rmb2.12092 |
work_keys_str_mv |
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1725981380787044352 |