Zerumbone, a Southeast Asian Ginger Sesquiterpene, Induced Apoptosis of Pancreatic Carcinoma Cells through p53 Signaling Pathway
Pancreatic carcinoma is one common cancer with gradually increasing incidence during the past several decades. However, currently the candidate drugs to suppress pancreatic cancer remain lacking. This research was carried out to investigate if zerumbone, a natural cyclic sesquiterpene isolated from...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2012-01-01
|
Series: | Evidence-Based Complementary and Alternative Medicine |
Online Access: | http://dx.doi.org/10.1155/2012/936030 |
id |
doaj-65408f05e11d48b086eee6d27c4ac36b |
---|---|
record_format |
Article |
spelling |
doaj-65408f05e11d48b086eee6d27c4ac36b2020-11-24T20:57:49ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882012-01-01201210.1155/2012/936030936030Zerumbone, a Southeast Asian Ginger Sesquiterpene, Induced Apoptosis of Pancreatic Carcinoma Cells through p53 Signaling PathwaySongyan Zhang0Qiaojing Liu1Yanju Liu2Hong Qiao3Yu Liu4Department of Hepatopancreatobiliary Surgery, The Third Affiliated Hospital of Harbin Medical University, Harbin 150040, ChinaDepartment of Laboratory Medicine, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaDepartment of Hepatopancreatobiliary Surgery, The Third Affiliated Hospital of Harbin Medical University, Harbin 150040, ChinaDepartment of Endocrinology, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, ChinaDepartment of Laboratory Medicine, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaPancreatic carcinoma is one common cancer with gradually increasing incidence during the past several decades. However, currently the candidate drugs to suppress pancreatic cancer remain lacking. This research was carried out to investigate if zerumbone, a natural cyclic sesquiterpene isolated from Zingiber zerumbet Smith, will produce the anticancer effects on pancreatic carcinoma cell lines. The results showed that zerumbone concentration, and time, dependently produced inhibitory actions on cell viability of PANC-1 cells. In addition, Hoechst 33342, AO/EB, TUNEL staining, and caspase-3 activity assay further showed that zerumbone induced apoptosis of PANC-1 cells. The expression of p53 protein was markedly upregulated, and the p21 level was also obviously elevated in zerumbone-treated PANC-1 cells. Moreover, ROS production was increased by about 149% in PANC-1 cells treated by zerumbone 30 μM. Zerumbone also produced the same antitumor activity in pancreatic carcinoma cell lines SW1990 and AsPC-1. In summary, we found that zerumbone was able to induce apoptosis of pancreatic carcinoma cell lines, indicating to be a promising treatment for pancreatic cancer.http://dx.doi.org/10.1155/2012/936030 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Songyan Zhang Qiaojing Liu Yanju Liu Hong Qiao Yu Liu |
spellingShingle |
Songyan Zhang Qiaojing Liu Yanju Liu Hong Qiao Yu Liu Zerumbone, a Southeast Asian Ginger Sesquiterpene, Induced Apoptosis of Pancreatic Carcinoma Cells through p53 Signaling Pathway Evidence-Based Complementary and Alternative Medicine |
author_facet |
Songyan Zhang Qiaojing Liu Yanju Liu Hong Qiao Yu Liu |
author_sort |
Songyan Zhang |
title |
Zerumbone, a Southeast Asian Ginger Sesquiterpene, Induced Apoptosis of Pancreatic Carcinoma Cells through p53 Signaling Pathway |
title_short |
Zerumbone, a Southeast Asian Ginger Sesquiterpene, Induced Apoptosis of Pancreatic Carcinoma Cells through p53 Signaling Pathway |
title_full |
Zerumbone, a Southeast Asian Ginger Sesquiterpene, Induced Apoptosis of Pancreatic Carcinoma Cells through p53 Signaling Pathway |
title_fullStr |
Zerumbone, a Southeast Asian Ginger Sesquiterpene, Induced Apoptosis of Pancreatic Carcinoma Cells through p53 Signaling Pathway |
title_full_unstemmed |
Zerumbone, a Southeast Asian Ginger Sesquiterpene, Induced Apoptosis of Pancreatic Carcinoma Cells through p53 Signaling Pathway |
title_sort |
zerumbone, a southeast asian ginger sesquiterpene, induced apoptosis of pancreatic carcinoma cells through p53 signaling pathway |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2012-01-01 |
description |
Pancreatic carcinoma is one common cancer with gradually increasing incidence during the past several decades. However, currently the candidate drugs to suppress pancreatic cancer remain lacking. This research was carried out to investigate if zerumbone, a natural cyclic sesquiterpene isolated from Zingiber zerumbet Smith, will produce the anticancer effects on pancreatic carcinoma cell lines. The results showed that zerumbone concentration, and time, dependently produced inhibitory actions on cell viability of PANC-1 cells. In addition, Hoechst 33342, AO/EB, TUNEL staining, and caspase-3 activity assay further showed that zerumbone induced apoptosis of PANC-1 cells. The expression of p53 protein was markedly upregulated, and the p21 level was also obviously elevated in zerumbone-treated PANC-1 cells. Moreover, ROS production was increased by about 149% in PANC-1 cells treated by zerumbone 30 μM. Zerumbone also produced the same antitumor activity in pancreatic carcinoma cell lines SW1990 and AsPC-1. In summary, we found that zerumbone was able to induce apoptosis of pancreatic carcinoma cell lines, indicating to be a promising treatment for pancreatic cancer. |
url |
http://dx.doi.org/10.1155/2012/936030 |
work_keys_str_mv |
AT songyanzhang zerumboneasoutheastasiangingersesquiterpeneinducedapoptosisofpancreaticcarcinomacellsthroughp53signalingpathway AT qiaojingliu zerumboneasoutheastasiangingersesquiterpeneinducedapoptosisofpancreaticcarcinomacellsthroughp53signalingpathway AT yanjuliu zerumboneasoutheastasiangingersesquiterpeneinducedapoptosisofpancreaticcarcinomacellsthroughp53signalingpathway AT hongqiao zerumboneasoutheastasiangingersesquiterpeneinducedapoptosisofpancreaticcarcinomacellsthroughp53signalingpathway AT yuliu zerumboneasoutheastasiangingersesquiterpeneinducedapoptosisofpancreaticcarcinomacellsthroughp53signalingpathway |
_version_ |
1716787365921423360 |