Photoperiodic diapause under the control of circadian clock genes in an insect

• Main Authors: Ikeno Tomoko, Tanaka Shinichi I, Numata Hideharu, Goto Shin G
• Format: Article
• Language: English
• Published: BMC 2010-09-01
• Series: BMC Biology
• Online Access: http://www.biomedcentral.com/1741-7007/8/116

<p>Abstract</p> <p>Background</p> <p>Most organisms have evolved a circadian clock in order to anticipate daily environmental changes and many of these organisms are also capable of sophisticated measurement of daylength (photoperiodism) that is used to regulate seasonal events such as diapause, migration and polymorphism. It has been generally accepted that the same elements are involved in both circadian (daily) and seasonal (annual) rhythms because both rely upon daily light-dark cycles. However, as reasonable as this sounds, there remains no conclusive evidence of such a molecular machinery in insects. We have approached this issue by using RNA interference (RNAi) in <it>Riptortus pedestris</it>.</p> <p>Results</p> <p>The cuticle deposition rhythm exhibited the major properties of circadian rhythms, indicating that the rhythm is regulated by a circadian clock. RNAi directed against the circadian clock genes of <it>period </it>and <it>cycle</it>, which are negative and positive regulators in the circadian clock, respectively, disrupted the cuticle deposition rhythm and distinct cuticle layers were produced by these RNAi. Simultaneously, <it>period </it>RNAi caused the insect to avert diapause under a diapause-inducing photoperiod whereas <it>cycle </it>RNAi induced diapause under a diapause-averting photoperiod. The expression patterns of juvenile hormone-regulated genes and the application of juvenile hormone analogue suggested that neither ovarian development itself nor a downstream cascade of juvenile hormone secretion, were disturbed by <it>period </it>and <it>cycle </it>RNAi.</p> <p>Conclusions</p> <p>This study revealed that the circadian clock genes are crucial not only for daily rhythms but also for photoperiodic diapause. RNAi directed against <it>period </it>and <it>cycle </it>had opposite effects not only in the circadian cuticle deposition rhythm but also in the photoperiodic diapause. These RNAi also had opposite effects on juvenile hormone-regulated gene expression. It is still possible that the circadian clock genes pleiotropically affect ovarian development but, based on these results, we suggest that the circadian clock operated by the circadian clock genes, <it>period </it>and <it>cycle</it>, governs seasonal timing as well as the daily rhythms.</p> <p>See Commentary: <url>http://www.biomedcentral.com/1741-7007/8/115</url></p>