Major QTLs, qARO1 and qARO9, Additively Regulate Adaxial Leaf Rolling in Rice
Moderate leaf rolling is considered optimal for the ideal plant type in rice (Oryza sativa L.), as it improves photosynthetic efficiency and, consequently, grain yield. Determining the genetic basis of leaf rolling via the identification of quantitative trait loci (QTLs) could facilitate the develop...
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doaj-6545f9385f7843b5a361da0d636175352021-02-23T08:02:11ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-02-011210.3389/fpls.2021.626523626523Major QTLs, qARO1 and qARO9, Additively Regulate Adaxial Leaf Rolling in RiceSu Jang0Sangrea Shim1Yoon Kyung Lee2Dongryung Lee3Hee-Jong Koh4Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, South KoreaDepartment of Chemistry, Plant Genomics and Breeding Institute, Seoul National University, Seoul, South KoreaDepartment of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, South KoreaKing Abdullah University of Science and Technology (KAUST), Division of Biological and Environmental Sciences and Engineering (BESE), Thuwal, Saudi ArabiaDepartment of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, South KoreaModerate leaf rolling is considered optimal for the ideal plant type in rice (Oryza sativa L.), as it improves photosynthetic efficiency and, consequently, grain yield. Determining the genetic basis of leaf rolling via the identification of quantitative trait loci (QTLs) could facilitate the development of high-yielding varieties. In this study, we identified three stable rice QTLs, qARO1, qARO5, and qARO9, which control adaxial leaf rolling in a recombinant inbred line (RIL) population derived from a cross between Tong 88-7 (T887) and Milyang 23 (M23), using high-density SNP markers. These QTLs controlled the rolling phenotype of both the flag leaf (FL) and secondary leaf (SL), and different allelic combinations of these QTLs led to a wide variation in the degree of leaf rolling. Additive gene actions of qARO1 and qARO9 on leaf rolling were observed in a backcross population. In addition, qARO1 (markers: 01id4854718 and 01asp4916781) and qARO9 (markers: 09id19650402 and 09id19740436) were successfully fine-mapped to approximately 60- and 90-kb intervals on chromosomes 1 and 9, respectively. Histological analysis of near-isogenic lines (NILs) revealed that qARO1 influences leaf thickness across the small vein, and qARO9 affects leaf thickness in the entire leaf and bulliform cell area, thus leading to adaxial leaf rolling. The results of this study advance our understanding of the genetic and molecular bases of adaxial leaf rolling, and this information can be used for the development of rice varieties with the ideal plant type.https://www.frontiersin.org/articles/10.3389/fpls.2021.626523/fullleaf rollingideotype breedingrice (Oryza sativa L.)quantitative trait lociideal plant typev-shape leaf |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Su Jang Sangrea Shim Yoon Kyung Lee Dongryung Lee Hee-Jong Koh |
spellingShingle |
Su Jang Sangrea Shim Yoon Kyung Lee Dongryung Lee Hee-Jong Koh Major QTLs, qARO1 and qARO9, Additively Regulate Adaxial Leaf Rolling in Rice Frontiers in Plant Science leaf rolling ideotype breeding rice (Oryza sativa L.) quantitative trait loci ideal plant type v-shape leaf |
author_facet |
Su Jang Sangrea Shim Yoon Kyung Lee Dongryung Lee Hee-Jong Koh |
author_sort |
Su Jang |
title |
Major QTLs, qARO1 and qARO9, Additively Regulate Adaxial Leaf Rolling in Rice |
title_short |
Major QTLs, qARO1 and qARO9, Additively Regulate Adaxial Leaf Rolling in Rice |
title_full |
Major QTLs, qARO1 and qARO9, Additively Regulate Adaxial Leaf Rolling in Rice |
title_fullStr |
Major QTLs, qARO1 and qARO9, Additively Regulate Adaxial Leaf Rolling in Rice |
title_full_unstemmed |
Major QTLs, qARO1 and qARO9, Additively Regulate Adaxial Leaf Rolling in Rice |
title_sort |
major qtls, qaro1 and qaro9, additively regulate adaxial leaf rolling in rice |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2021-02-01 |
description |
Moderate leaf rolling is considered optimal for the ideal plant type in rice (Oryza sativa L.), as it improves photosynthetic efficiency and, consequently, grain yield. Determining the genetic basis of leaf rolling via the identification of quantitative trait loci (QTLs) could facilitate the development of high-yielding varieties. In this study, we identified three stable rice QTLs, qARO1, qARO5, and qARO9, which control adaxial leaf rolling in a recombinant inbred line (RIL) population derived from a cross between Tong 88-7 (T887) and Milyang 23 (M23), using high-density SNP markers. These QTLs controlled the rolling phenotype of both the flag leaf (FL) and secondary leaf (SL), and different allelic combinations of these QTLs led to a wide variation in the degree of leaf rolling. Additive gene actions of qARO1 and qARO9 on leaf rolling were observed in a backcross population. In addition, qARO1 (markers: 01id4854718 and 01asp4916781) and qARO9 (markers: 09id19650402 and 09id19740436) were successfully fine-mapped to approximately 60- and 90-kb intervals on chromosomes 1 and 9, respectively. Histological analysis of near-isogenic lines (NILs) revealed that qARO1 influences leaf thickness across the small vein, and qARO9 affects leaf thickness in the entire leaf and bulliform cell area, thus leading to adaxial leaf rolling. The results of this study advance our understanding of the genetic and molecular bases of adaxial leaf rolling, and this information can be used for the development of rice varieties with the ideal plant type. |
topic |
leaf rolling ideotype breeding rice (Oryza sativa L.) quantitative trait loci ideal plant type v-shape leaf |
url |
https://www.frontiersin.org/articles/10.3389/fpls.2021.626523/full |
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