An in vitro study on anti-sickling activity of Terminalia arjuna and Terminalia bellirica
Background & Aim:Combretaceae family is known for efficacy in the management of sickle cell disorder (SCD). Based on the earlier reports of anti-sickling property of Terminalia genus the study investigates in vitro antisickling properties of Terminalia arjuna and Terminalia bellirica. Exp...
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Islamic Azad University, Shahrekord Branch
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doaj-654babcf9fa24fb1bf88ba203a5832132020-11-24T22:23:48ZengIslamic Azad University, Shahrekord BranchJournal of Herbal Drugs2008-88842476-53922017-07-01811710.18869/JHD.2017.1An in vitro study on anti-sickling activity of Terminalia arjuna and Terminalia belliricaAnand Dilip Firodiya0Abin Mani 1Vijay Thawani2Human Genetic Lab, Centre for Scientific Research and Development, Peoples University, Bhopal-462037, Madhya Pradesh, IndiaHuman Genetic Lab, Centre for Scientific Research and Development, Peoples University, Bhopal-462037, Madhya Pradesh, IndiaHuman Genetic Lab, Centre for Scientific Research and Development, Peoples University, Bhopal-462037, Madhya Pradesh, IndiaBackground & Aim:Combretaceae family is known for efficacy in the management of sickle cell disorder (SCD). Based on the earlier reports of anti-sickling property of Terminalia genus the study investigates in vitro antisickling properties of Terminalia arjuna and Terminalia bellirica. Experimental: Anti-sickling assay of methanolic and aqueous extract of T. arjuna and T. bellirica on sickled RBCs were evaluated at different concentrations. The osmotic fragility test was done to explore the effect of extracts on haemoglobin S solubility and sickle cell membrane stability. Additionally antioxidant activity and phytochemical analysis of extracts was evaluated. Results: Treatment with the plant extracts at different concentrations showed decrease in percentage of sickled cells. Extract of T. arjuna had a significantly higher (p < 0.05) antisickling activity at the tested concentrations of 10 mg/ml. The methanolic extract at 10 mg/ml showed the best cell membrane stability as compared to T. bellirica extract. It was observed that, T. bellirica extract was deficient of alkaloid and tannins. Moreover, all extracts/fractions showed antioxidant activity, although lesser as compared to the standard. Recommended applications/industries: It was observed that the leaf extracts of T. arjuna has a significant antisickling effect, hence it can be potentially used in clinical management of SCD. The bioactive compounds can be used to supplement as well as management drug for sickle cell disease.http://jhd.iaushk.ac.ir/article_25336.htmlSickle cell diseasemethanolic extractPhytochemicalosmotic fragilityAntioxidant |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anand Dilip Firodiya Abin Mani Vijay Thawani |
spellingShingle |
Anand Dilip Firodiya Abin Mani Vijay Thawani An in vitro study on anti-sickling activity of Terminalia arjuna and Terminalia bellirica Journal of Herbal Drugs Sickle cell disease methanolic extract Phytochemical osmotic fragility Antioxidant |
author_facet |
Anand Dilip Firodiya Abin Mani Vijay Thawani |
author_sort |
Anand Dilip Firodiya |
title |
An in vitro study on anti-sickling activity of Terminalia arjuna and Terminalia bellirica |
title_short |
An in vitro study on anti-sickling activity of Terminalia arjuna and Terminalia bellirica |
title_full |
An in vitro study on anti-sickling activity of Terminalia arjuna and Terminalia bellirica |
title_fullStr |
An in vitro study on anti-sickling activity of Terminalia arjuna and Terminalia bellirica |
title_full_unstemmed |
An in vitro study on anti-sickling activity of Terminalia arjuna and Terminalia bellirica |
title_sort |
in vitro study on anti-sickling activity of terminalia arjuna and terminalia bellirica |
publisher |
Islamic Azad University, Shahrekord Branch |
series |
Journal of Herbal Drugs |
issn |
2008-8884 2476-5392 |
publishDate |
2017-07-01 |
description |
Background & Aim:Combretaceae family is known for efficacy in the management of sickle cell disorder (SCD). Based on the earlier reports of anti-sickling property of Terminalia genus the study investigates in vitro antisickling properties of Terminalia arjuna and Terminalia bellirica.
Experimental: Anti-sickling assay of methanolic and aqueous extract of T. arjuna and T. bellirica on sickled RBCs were evaluated at different concentrations. The osmotic fragility test was done to explore the effect of extracts on haemoglobin S solubility and sickle cell membrane stability. Additionally antioxidant activity and phytochemical analysis of extracts was evaluated.
Results: Treatment with the plant extracts at different concentrations showed decrease in percentage of sickled cells. Extract of T. arjuna had a significantly higher (p < 0.05) antisickling activity at the tested concentrations of 10 mg/ml. The methanolic extract at 10 mg/ml showed the best cell membrane stability as compared to T. bellirica extract. It was observed that, T. bellirica extract was deficient of alkaloid and tannins. Moreover, all extracts/fractions showed antioxidant activity, although lesser as compared to the standard.
Recommended applications/industries: It was observed that the leaf extracts of T. arjuna has a significant antisickling effect, hence it can be potentially used in clinical management of SCD. The bioactive compounds can be used to supplement as well as management drug for sickle cell disease. |
topic |
Sickle cell disease methanolic extract Phytochemical osmotic fragility Antioxidant |
url |
http://jhd.iaushk.ac.ir/article_25336.html |
work_keys_str_mv |
AT ananddilipfirodiya aninvitrostudyonantisicklingactivityofterminaliaarjunaandterminaliabellirica AT abinmani aninvitrostudyonantisicklingactivityofterminaliaarjunaandterminaliabellirica AT vijaythawani aninvitrostudyonantisicklingactivityofterminaliaarjunaandterminaliabellirica AT ananddilipfirodiya invitrostudyonantisicklingactivityofterminaliaarjunaandterminaliabellirica AT abinmani invitrostudyonantisicklingactivityofterminaliaarjunaandterminaliabellirica AT vijaythawani invitrostudyonantisicklingactivityofterminaliaarjunaandterminaliabellirica |
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