Cryo-EM structure of the homohexameric T3SS ATPase-central stalk complex reveals rotary ATPase-like asymmetry

Many Gram-negative bacteria rely on a type III secretion system (T3SS) for their pathogenicity. Here authors present the cryo-EM structure of the E.coli T3SS ATPase-central stalk complex, which forms a homohexameric, asymmetric pore with different functional states.

Bibliographic Details
Main Authors:	Dorothy D. Majewski, Liam J. Worrall, Chuan Hong, Claire E. Atkinson, Marija Vuckovic, Nobuhiko Watanabe, Zhiheng Yu, Natalie C. J. Strynadka
Format:	Article
Language:	English
Published:	Nature Publishing Group 2019-02-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-019-08477-7

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spelling	doaj-655ed92072524851b04d71448b77be1e2021-05-11T12:18:57ZengNature Publishing GroupNature Communications2041-17232019-02-0110111210.1038/s41467-019-08477-7Cryo-EM structure of the homohexameric T3SS ATPase-central stalk complex reveals rotary ATPase-like asymmetryDorothy D. Majewski0Liam J. Worrall1Chuan Hong2Claire E. Atkinson3Marija Vuckovic4Nobuhiko Watanabe5Zhiheng Yu6Natalie C. J. Strynadka7Department of Biochemistry and Molecular Biology and the Center for Blood Research, University of British ColumbiaDepartment of Biochemistry and Molecular Biology and the Center for Blood Research, University of British ColumbiaCryoEM Shared Resources, Howard Hughes Medical InstituteDepartment of Biochemistry and Molecular Biology and the Center for Blood Research, University of British ColumbiaDepartment of Biochemistry and Molecular Biology and the Center for Blood Research, University of British ColumbiaDepartment of Biochemistry and Molecular Biology and the Center for Blood Research, University of British ColumbiaCryoEM Shared Resources, Howard Hughes Medical InstituteDepartment of Biochemistry and Molecular Biology and the Center for Blood Research, University of British ColumbiaMany Gram-negative bacteria rely on a type III secretion system (T3SS) for their pathogenicity. Here authors present the cryo-EM structure of the E.coli T3SS ATPase-central stalk complex, which forms a homohexameric, asymmetric pore with different functional states.https://doi.org/10.1038/s41467-019-08477-7
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Dorothy D. Majewski Liam J. Worrall Chuan Hong Claire E. Atkinson Marija Vuckovic Nobuhiko Watanabe Zhiheng Yu Natalie C. J. Strynadka
spellingShingle	Dorothy D. Majewski Liam J. Worrall Chuan Hong Claire E. Atkinson Marija Vuckovic Nobuhiko Watanabe Zhiheng Yu Natalie C. J. Strynadka Cryo-EM structure of the homohexameric T3SS ATPase-central stalk complex reveals rotary ATPase-like asymmetry Nature Communications
author_facet	Dorothy D. Majewski Liam J. Worrall Chuan Hong Claire E. Atkinson Marija Vuckovic Nobuhiko Watanabe Zhiheng Yu Natalie C. J. Strynadka
author_sort	Dorothy D. Majewski
title	Cryo-EM structure of the homohexameric T3SS ATPase-central stalk complex reveals rotary ATPase-like asymmetry
title_short	Cryo-EM structure of the homohexameric T3SS ATPase-central stalk complex reveals rotary ATPase-like asymmetry
title_full	Cryo-EM structure of the homohexameric T3SS ATPase-central stalk complex reveals rotary ATPase-like asymmetry
title_fullStr	Cryo-EM structure of the homohexameric T3SS ATPase-central stalk complex reveals rotary ATPase-like asymmetry
title_full_unstemmed	Cryo-EM structure of the homohexameric T3SS ATPase-central stalk complex reveals rotary ATPase-like asymmetry
title_sort	cryo-em structure of the homohexameric t3ss atpase-central stalk complex reveals rotary atpase-like asymmetry
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2019-02-01
description	Many Gram-negative bacteria rely on a type III secretion system (T3SS) for their pathogenicity. Here authors present the cryo-EM structure of the E.coli T3SS ATPase-central stalk complex, which forms a homohexameric, asymmetric pore with different functional states.
url	https://doi.org/10.1038/s41467-019-08477-7
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Cryo-EM structure of the homohexameric T3SS ATPase-central stalk complex reveals rotary ATPase-like asymmetry

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