MICRO-TEST SYSTEM FOR RAPID ISOLATION AND IDENTIFICATION OF CANDIDA SPECIES IN URINARY TRACT INFECTIONS

• Main Authors: Nicolae Puşcaş, Greta Bălan, Olga Burduniuc
• Format: Article
• Language: English
• Published: “Francisc I. Rainer” Anthropology Institute of the Romanian Academy. 2017-07-01
• Series: Anthropological Researches and Studies
• Subjects: Candida species; fast identification; culture medium; urinary tract infections
• Online Access: http://www.journalstudiesanthropology.ro/en/micro-test-system-for-rapid-isolation-and-identification-of-candida-species-in-urinary-tract-infections/a77/
• ISSN: 2360-3445; 2360-3445

Objectives. We tried to create a culture medium and a method used to rapidly isolate and identify the yeasts of Candida genus. Material and Methods. The isolation and identification of Candida genus yeasts have been accomplished using the micro-test system alongside the classical method. The strains of reference were collected from the Bacteriological Laboratory of the National Centre for Public Health. Results. Choosing the culture medium for a prompt isolation of the germs represents a primordial element for determining the correct diagnosis. The elaborated medium increases the sensibility, selectivity and the specificity of the test used to identify Candida genus yeasts. The method involves the insertion of the material into the culture medium followed by a further incubation and identification of the Candida genus yeasts. Conclusions. We have created a culture medium that allows a fast identification of the Candida genus yeasts. The medium is sensitive, economical and easy to use, as well as accessible for the microbiological labs of various levels. Comparing to the usual practices, the elaborated method gives us the possibility to obtain a result in 9 hours after the inoculation of the pathological material.