Epigenetic Regulation of Cytokine Production in Human Amnion and Villous Placenta
The mechanisms of human preterm labour appear inextricably linked to cytokine biosynthesis by gestational tissues. In turn, cytokine production by gestational tissues has been shown to be regulated by epigenetic mechanisms. In this paper, we demonstrate that cytokine production in gestational tissue...
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2012-01-01
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Online Access: | http://dx.doi.org/10.1155/2012/159709 |
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doaj-65a6d29f13144c69a5c0ee58e5d9cc922020-11-24T22:30:42ZengHindawi LimitedMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/159709159709Epigenetic Regulation of Cytokine Production in Human Amnion and Villous PlacentaMurray D. Mitchell0Anna P. Ponnampalam1Gregory E. Rice2The University of Queensland Centre for Clinical Research, Royal Brisbane Hospital, Building 71/918, Herston, QLD 4029, AustraliaThe Liggins Institute, University of Auckland, 2-6 Park Avenue, Private Bag 92019, Auckland 1023, New ZealandThe University of Queensland Centre for Clinical Research, Royal Brisbane Hospital, Building 71/918, Herston, QLD 4029, AustraliaThe mechanisms of human preterm labour appear inextricably linked to cytokine biosynthesis by gestational tissues. In turn, cytokine production by gestational tissues has been shown to be regulated by epigenetic mechanisms. In this paper, we demonstrate that cytokine production in gestational tissues is regulated epigenetically in a tissue-specific manner. Furthermore, we show that treatment with a histone deacetylation inhibitor can partially abrogate LPS-stimulated TNFα production in villous placenta but not amnion. LPS treatment significantly (𝑃<0.05) increased the production of IL-1β (~10–34-fold), TNFα (~23–>100-fold) and IL10 (~6–10-fold) after 24 h of treatment in villous explants, as expected. There were no significant LPS effects on IL1Ra production. AZA treatment did not have any significant effect on any cytokines' production tested either alone or in combination with LPS. Interestingly, however, the stimulatory effects of LPS on TNFα production were partially mitigated (𝑃<0.05) by TSA treatment in villous explants. We suggest caution in the consideration of histone deacetylation inhibitors in pregnancy due to the different responses in gestational tissues.http://dx.doi.org/10.1155/2012/159709 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Murray D. Mitchell Anna P. Ponnampalam Gregory E. Rice |
spellingShingle |
Murray D. Mitchell Anna P. Ponnampalam Gregory E. Rice Epigenetic Regulation of Cytokine Production in Human Amnion and Villous Placenta Mediators of Inflammation |
author_facet |
Murray D. Mitchell Anna P. Ponnampalam Gregory E. Rice |
author_sort |
Murray D. Mitchell |
title |
Epigenetic Regulation of Cytokine Production in Human Amnion and Villous Placenta |
title_short |
Epigenetic Regulation of Cytokine Production in Human Amnion and Villous Placenta |
title_full |
Epigenetic Regulation of Cytokine Production in Human Amnion and Villous Placenta |
title_fullStr |
Epigenetic Regulation of Cytokine Production in Human Amnion and Villous Placenta |
title_full_unstemmed |
Epigenetic Regulation of Cytokine Production in Human Amnion and Villous Placenta |
title_sort |
epigenetic regulation of cytokine production in human amnion and villous placenta |
publisher |
Hindawi Limited |
series |
Mediators of Inflammation |
issn |
0962-9351 1466-1861 |
publishDate |
2012-01-01 |
description |
The mechanisms of human preterm labour appear inextricably linked to cytokine biosynthesis by gestational tissues. In turn, cytokine production by gestational tissues has been shown to be regulated by epigenetic mechanisms. In this paper, we demonstrate that cytokine production in gestational tissues is regulated epigenetically in a tissue-specific manner. Furthermore, we show that treatment with a histone deacetylation inhibitor can partially abrogate LPS-stimulated TNFα production in villous placenta but not amnion. LPS treatment significantly (𝑃<0.05) increased the production of IL-1β (~10–34-fold), TNFα (~23–>100-fold) and IL10 (~6–10-fold) after 24 h of treatment in villous explants, as expected. There were no significant LPS effects on IL1Ra production. AZA treatment did not have any significant effect on any cytokines' production tested either alone or in combination with LPS. Interestingly, however, the stimulatory effects of LPS on TNFα production were partially mitigated (𝑃<0.05) by TSA treatment in villous explants. We suggest caution in the consideration of histone deacetylation inhibitors in pregnancy due to the different responses in gestational tissues. |
url |
http://dx.doi.org/10.1155/2012/159709 |
work_keys_str_mv |
AT murraydmitchell epigeneticregulationofcytokineproductioninhumanamnionandvillousplacenta AT annapponnampalam epigeneticregulationofcytokineproductioninhumanamnionandvillousplacenta AT gregoryerice epigeneticregulationofcytokineproductioninhumanamnionandvillousplacenta |
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