Pneumoperitoneum induces morphological alterations in the rat testicle

• Main Authors: Carina Teixeira Ribeiro, Diogo Benchimol De Souza, Jorge Luiz Medeiros Jr., Waldemar Silva Costa, Marco Aurélio Pereira-Sampaio, Francisco José Barcellos Sampaio
• Format: Article
• Language: English
• Published: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2013-06-01
• Series: Acta Cirurgica Brasileira
• Subjects: Pneumoperitoneum; Testis; Histology; Rats
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000600003
• ISSN: 0102-8650; 1678-2674

PURPOSE:To investigate the seminiferous tubule histological morphology after an 8 mmHg pneumoperitoneum in the rat model. METHODS: Fourteen rats were divided into two groups: a Sham group submitted to anesthesia and a pneumoperitoneum (Pp) group submitted to abdominal insufflation at 8 mmHg during three hours, followed by desuflation. All rats were killed after six weeks, testicles were collected and evaluated for the tubule diameter, germinative epithelium height and Johnsen´s score. Means were compared by using the Student's-t-test. RESULTS:The seminiferous tubule diameter was diminished by 11.3% in the group submitted to pneumoperitoneum (p<0.05). No significant difference was found among the groups when analyzing the epithelium height and Johnsen´s score. CONCLUSION:In the rat model, the seminiferous tubules present structural alterations when subjected to pneumoperitoneum of 8 mmHg during three hours.