Isolation of Eimeria oocysts from soil samples: a simple method described in detail
Molecular methods are currently available to detect parasitic DNA in soil, but do not replace the need for direct parasitological methods that can identify the extent of contamination. This report describes an inexpensive and fast flotation method for retrieving Eimeria oocysts from soil that requir...
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Estonian Academic Agricultural Society
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doaj-65cf3960597c4471902628db64084a242020-11-24T22:44:12ZengEstonian Academic Agricultural SocietyAgraarteadus1024-08452228-48932014-12-012527781Isolation of Eimeria oocysts from soil samples: a simple method described in detailBrian Lassen0Triin Lepik1 Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 62, 51014 Tartu, Estonia Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 62, 51014 Tartu, Estonia Molecular methods are currently available to detect parasitic DNA in soil, but do not replace the need for direct parasitological methods that can identify the extent of contamination. This report describes an inexpensive and fast flotation method for retrieving Eimeria oocysts from soil that requires few tools. Soil samples were experimentally contaminated with 50,000 E. bovis oocysts and compared with soil-free controls. A separate experiment tested the effect of mechanical stress by shaking the soil and oocyst mix 0, 1, 5, and 10 times before attempting to retrieve the oocysts. The percentage of oocysts retrieved using the flotation method was 22%, which was similar to the results obtained with previously described, more labour-intensive methods. The presence of soil reduced the percentage of oocysts that could be retrieved by 23%. A single shake of the oocysts and soil mixture was sufficient to significantly reduce the recoverable proportion of oocysts. The results indicated that the developed simple method can be applied to recover oocysts, and that gentle handling of soil samples prior to oocyst isolation is important. http://agrt.emu.ee/pdf/2014_2_lassen.pdf Eimeriasoildiagnosiscontaminationflotationmethod |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Brian Lassen Triin Lepik |
spellingShingle |
Brian Lassen Triin Lepik Isolation of Eimeria oocysts from soil samples: a simple method described in detail Agraarteadus Eimeria soil diagnosis contamination flotation method |
author_facet |
Brian Lassen Triin Lepik |
author_sort |
Brian Lassen |
title |
Isolation of Eimeria oocysts from soil samples: a simple method described in detail |
title_short |
Isolation of Eimeria oocysts from soil samples: a simple method described in detail |
title_full |
Isolation of Eimeria oocysts from soil samples: a simple method described in detail |
title_fullStr |
Isolation of Eimeria oocysts from soil samples: a simple method described in detail |
title_full_unstemmed |
Isolation of Eimeria oocysts from soil samples: a simple method described in detail |
title_sort |
isolation of eimeria oocysts from soil samples: a simple method described in detail |
publisher |
Estonian Academic Agricultural Society |
series |
Agraarteadus |
issn |
1024-0845 2228-4893 |
publishDate |
2014-12-01 |
description |
Molecular methods are currently available to detect parasitic DNA in soil, but do not replace the need for direct parasitological methods that can identify the extent of contamination. This report describes an inexpensive and fast flotation method for retrieving Eimeria oocysts from soil that requires few tools. Soil samples were experimentally contaminated with 50,000 E. bovis oocysts and compared with soil-free controls. A separate experiment tested the effect of mechanical stress by shaking the soil and oocyst mix 0, 1, 5, and 10 times before attempting to retrieve the oocysts. The percentage of oocysts retrieved using the flotation method was 22%, which was similar to the results obtained with previously described, more labour-intensive methods. The presence of soil reduced the percentage of oocysts that could be retrieved by 23%. A single shake of the oocysts and soil mixture was sufficient to significantly reduce the recoverable proportion of oocysts. The results indicated that the developed simple method can be applied to recover oocysts, and that gentle handling of soil samples prior to oocyst isolation is important. |
topic |
Eimeria soil diagnosis contamination flotation method |
url |
http://agrt.emu.ee/pdf/2014_2_lassen.pdf
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work_keys_str_mv |
AT brianlassen isolationofeimeriaoocystsfromsoilsamplesasimplemethoddescribedindetail AT triinlepik isolationofeimeriaoocystsfromsoilsamplesasimplemethoddescribedindetail |
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