LncRNA PVT1 Facilitates Proliferation, Migration and Invasion of NSCLC Cells via miR-551b/FGFR1 Axis

• Main Authors: Wang X, Cheng Z, Dai L, Jiang T, Li P, Jia L, Jing X, An L, Liu M, Wu S, Wang Y
• Format: Article
• Language: English
• Published: Dove Medical Press 2021-06-01
• Series: OncoTargets and Therapy
• Subjects: lncrna pvt; mir-551b; fgfr1; nsclc
• Online Access: https://www.dovepress.com/lncrna-pvt1-facilitates-proliferation-migration-and-invasion-of-nsclc--peer-reviewed-fulltext-article-OTT

Xi Wang, Zhe Cheng, Lingling Dai, Tianci Jiang, Pengfei Li, Liuqun Jia, Xiaogang Jing, Lin An, Meng Liu, Shujun Wu, Yu Wang Department of Respiration, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, People’s Republic of ChinaCorrespondence: Zhe ChengDepartment of Respiration, The First Affiliated Hospital of Zhengzhou University, Jianshe East Road No. 1, Zhengzhou, 450052, Henan, People’s Republic of ChinaEmail [e-mail]: Long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) plays a crucial role in non-small cell lung cancer (NSCLC). Nonetheless, regulatory effects of PVT1 on functions of NSCLC cells remain blurry.Methods: Relative expression levels of PVT1, miR-551b and FGFR1 mRNA in tumor tissues and cells were examined employing quantitative real-time polymerase chain reaction (qRT-PCR); CCK-8 and BrdU assays were utilized for measuring cell viability and proliferation of H1299 and A549 cells; cell migration and invasion were detected deploying Transwell assay; dual-luciferase assay was used for the validation of binding sequence between PVT1 and miR-551b. FGFR1 expression in protein level was quantified employing Western blot.Results: PVT1 was highly expressed in NSCLC tissues and cell lines, whereas miR-551b expression was down-regulated. Overexpression of PVT1 potentiated viability, proliferation, migration and invasion of NSCLC cells while miR-551b inhibited the biological behaviors mentioned above. MiR-551b was predicted and then confirmed as a direct downstream target of PVT1. Meanwhile, a negative correlation was observed between PVT1 expression and miR-551b expression in NSCLC tissues. Besides, PVT1 could increase FGFR1 expression by repressing miR-551b expression.Conclusion: PVT1 promotes the proliferation, migration and invasion of NSCLC cells by indirectly mediating FGFR1 via targeting miR-551b.Keywords: lncRNA PVT, miR-551b, FGFR1, NSCLC