Preliminary evidence of the association between DNAm and orbital volumetry in GO
Background: The pathogenesis underlying the alterations of orbital architecture in Graves’ orbitopathy (GO) is not yet fully understood. The present study aimed to investigate the association of DNA methylation in peripheral blood and orbital volumetry in Chinese patients with GO. Methods: A total...
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Bioscientifica
2020-07-01
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doaj-65f8f33ddcec4f82b2f64b58d6b6aa3e2020-11-25T03:29:21ZengBioscientificaEndocrine Connections2049-36142049-36142020-07-0197617626https://doi.org/10.1530/EC-20-0147Preliminary evidence of the association between DNAm and orbital volumetry in GOYa-Fen Hu0Lin Hua1Xiu Tuo2Ting-Ting Shi3Yi-Lin Yang4Yun-Fu Liu5Zhong-Yu Yan6Zhong Xin7Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing, China; Department of Endocrinology, The People’s Hospital of Daxing District, Beijing, ChinaDepartment of Mathematics, School of Biomedical Engineering, Capital Medical University, Beijing, ChinaDepartment of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing, ChinaDepartment of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing, ChinaDepartment of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing, ChinaDepartment of Radiology, Beijing Tongren Hospital, Capital Medical University, Beijing, ChinaDepartment of Radiology, Beijing Tongren Hospital, Capital Medical University, Beijing, ChinaDepartment of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing, ChinaBackground: The pathogenesis underlying the alterations of orbital architecture in Graves’ orbitopathy (GO) is not yet fully understood. The present study aimed to investigate the association of DNA methylation in peripheral blood and orbital volumetry in Chinese patients with GO. Methods: A total of 35 GO subjects (70 orbits) were subjected to CT scan. The total cross-sectional area of the extraocular muscles (orbital muscles, OM), total orbit area (TOA), and the exophthalmometry were measured and OM/TOA ratio was calculated. Targeted bisulfite sequencing was performed on seven candidate genes. Results: No significant correlation was established between the DNA meth ylation levels of these genes and exophthalmometry. The MBP methylation level was found to be correlated with OM/TOA ratio (P < 0.05). Multiple linear regression analysis on parameters including age, sex, TRAb, duration of GO, and DNA methylation levels of seven genes with OM/TOA ratio confirmed that MBP and OM/TOA ratio had a significant correlation (P < 0.05). The partial least squares analysis showed that the top three genes with the highest loadings were MBP, BOLL, and BECN1 and that OM/TOA ratio affected the DNA methylation block than exophthalmometry. Conclusions: This study provided preliminary evidence that MBP is a potential gene associated with OM enlargement in GO patients according to the combination of DNA methylation sequencing and orbital CT measurement. https://ec.bioscientifica.com/view/journals/ec/9/7/EC-20-0147.xmlgraves’ orbitopathyextraocular musclesorbitdna methylation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ya-Fen Hu Lin Hua Xiu Tuo Ting-Ting Shi Yi-Lin Yang Yun-Fu Liu Zhong-Yu Yan Zhong Xin |
spellingShingle |
Ya-Fen Hu Lin Hua Xiu Tuo Ting-Ting Shi Yi-Lin Yang Yun-Fu Liu Zhong-Yu Yan Zhong Xin Preliminary evidence of the association between DNAm and orbital volumetry in GO Endocrine Connections graves’ orbitopathy extraocular muscles orbit dna methylation |
author_facet |
Ya-Fen Hu Lin Hua Xiu Tuo Ting-Ting Shi Yi-Lin Yang Yun-Fu Liu Zhong-Yu Yan Zhong Xin |
author_sort |
Ya-Fen Hu |
title |
Preliminary evidence of the association between DNAm and orbital volumetry in GO |
title_short |
Preliminary evidence of the association between DNAm and orbital volumetry in GO |
title_full |
Preliminary evidence of the association between DNAm and orbital volumetry in GO |
title_fullStr |
Preliminary evidence of the association between DNAm and orbital volumetry in GO |
title_full_unstemmed |
Preliminary evidence of the association between DNAm and orbital volumetry in GO |
title_sort |
preliminary evidence of the association between dnam and orbital volumetry in go |
publisher |
Bioscientifica |
series |
Endocrine Connections |
issn |
2049-3614 2049-3614 |
publishDate |
2020-07-01 |
description |
Background: The pathogenesis underlying the alterations of orbital architecture in Graves’ orbitopathy (GO) is not yet fully understood. The present study aimed to investigate the association of DNA methylation in peripheral blood and orbital volumetry in Chinese patients with GO.
Methods: A total of 35 GO subjects (70 orbits) were subjected to CT scan. The total cross-sectional area of the extraocular muscles (orbital muscles, OM), total orbit area (TOA), and the exophthalmometry were measured and OM/TOA ratio was calculated. Targeted bisulfite sequencing was performed on seven candidate genes.
Results: No significant correlation was established between the DNA meth ylation levels of these genes and exophthalmometry. The MBP methylation level was found to be correlated with OM/TOA ratio (P < 0.05). Multiple linear regression analysis on parameters including age, sex, TRAb, duration of GO, and DNA methylation levels of seven genes with OM/TOA ratio confirmed that MBP and OM/TOA ratio had a significant correlation (P < 0.05). The partial least squares analysis showed that the top three genes with the highest loadings were MBP, BOLL, and BECN1 and that OM/TOA ratio affected the DNA methylation block than exophthalmometry.
Conclusions: This study provided preliminary evidence that MBP is a potential gene associated with OM enlargement in GO patients according to the combination of DNA methylation sequencing and orbital CT measurement.
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topic |
graves’ orbitopathy extraocular muscles orbit dna methylation |
url |
https://ec.bioscientifica.com/view/journals/ec/9/7/EC-20-0147.xml |
work_keys_str_mv |
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