Antimicrobial Activity of an Amnion-Chorion Membrane to Oral Microbes
Objective. The aim of this study was to evaluate wound biomodification by assessing antimicrobial properties present within a human-derived composite amnion-chorion membrane (ACM). Methods. Membranes analyzed were the human-derived ACM BioXclude™ and the porcine-derived collagen membrane Bio-Gide®....
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Series: | International Journal of Dentistry |
Online Access: | http://dx.doi.org/10.1155/2019/1269534 |
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doaj-6610b3e220504c3d93e38469458df3312020-11-25T00:45:56ZengHindawi LimitedInternational Journal of Dentistry1687-87281687-87362019-01-01201910.1155/2019/12695341269534Antimicrobial Activity of an Amnion-Chorion Membrane to Oral MicrobesHaroon Ashraf0Kerri Font1Charles Powell2Michael Schurr3Department of Surgical Dentistry, Division of Periodontics, University of Colorado School of Dental Medicine, Aurora, CO, USADepartment of Surgical Dentistry, Division of Periodontics, University of Colorado School of Dental Medicine, Aurora, CO, USADepartment of Surgical Dentistry, Division of Periodontics, University of Colorado School of Dental Medicine, Aurora, CO, USADepartment of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO, USAObjective. The aim of this study was to evaluate wound biomodification by assessing antimicrobial properties present within a human-derived composite amnion-chorion membrane (ACM). Methods. Membranes analyzed were the human-derived ACM BioXclude™ and the porcine-derived collagen membrane Bio-Gide®. Paper discs with and without tetracycline served as positive and negative controls, respectively. The same number of colony-forming units per milliliter for each bacterial species (Aggregatibacter actinomycetemcomitans, Streptococcus mutans, and Streptococcus oralis) was inoculated on each of the discs. Discs from each group were removed at 12 and 24 hours and sonicated to remove the bacteria off the membranes. A serial dilution was performed to quantify bacterial growth. Results. The ACM inhibited growth at all time points, with all bacterial strains, identical to the negative control tetracycline discs. The collagen membrane and positive controls did not inhibit growth of any of the bacterial species throughout the 24-hour study period. P<0.05 for microbial growth on ACM or negative control vs. either collagen membrane or positive control. Conclusion. ACM was proven to be as bactericidal as paper discs inoculated with tetracycline at its minimum bactericidal concentration. The ACM bactericidal property may be beneficial in the early wound healing process.http://dx.doi.org/10.1155/2019/1269534 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Haroon Ashraf Kerri Font Charles Powell Michael Schurr |
spellingShingle |
Haroon Ashraf Kerri Font Charles Powell Michael Schurr Antimicrobial Activity of an Amnion-Chorion Membrane to Oral Microbes International Journal of Dentistry |
author_facet |
Haroon Ashraf Kerri Font Charles Powell Michael Schurr |
author_sort |
Haroon Ashraf |
title |
Antimicrobial Activity of an Amnion-Chorion Membrane to Oral Microbes |
title_short |
Antimicrobial Activity of an Amnion-Chorion Membrane to Oral Microbes |
title_full |
Antimicrobial Activity of an Amnion-Chorion Membrane to Oral Microbes |
title_fullStr |
Antimicrobial Activity of an Amnion-Chorion Membrane to Oral Microbes |
title_full_unstemmed |
Antimicrobial Activity of an Amnion-Chorion Membrane to Oral Microbes |
title_sort |
antimicrobial activity of an amnion-chorion membrane to oral microbes |
publisher |
Hindawi Limited |
series |
International Journal of Dentistry |
issn |
1687-8728 1687-8736 |
publishDate |
2019-01-01 |
description |
Objective. The aim of this study was to evaluate wound biomodification by assessing antimicrobial properties present within a human-derived composite amnion-chorion membrane (ACM). Methods. Membranes analyzed were the human-derived ACM BioXclude™ and the porcine-derived collagen membrane Bio-Gide®. Paper discs with and without tetracycline served as positive and negative controls, respectively. The same number of colony-forming units per milliliter for each bacterial species (Aggregatibacter actinomycetemcomitans, Streptococcus mutans, and Streptococcus oralis) was inoculated on each of the discs. Discs from each group were removed at 12 and 24 hours and sonicated to remove the bacteria off the membranes. A serial dilution was performed to quantify bacterial growth. Results. The ACM inhibited growth at all time points, with all bacterial strains, identical to the negative control tetracycline discs. The collagen membrane and positive controls did not inhibit growth of any of the bacterial species throughout the 24-hour study period. P<0.05 for microbial growth on ACM or negative control vs. either collagen membrane or positive control. Conclusion. ACM was proven to be as bactericidal as paper discs inoculated with tetracycline at its minimum bactericidal concentration. The ACM bactericidal property may be beneficial in the early wound healing process. |
url |
http://dx.doi.org/10.1155/2019/1269534 |
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