Mitochondrial Genome Structures and Phylogenetic Analyses of Two Tropical Characidae Fishes
The Characidae family contains the largest number of tropical fish species. Morphological similarities make species identification difficult within this family. Here, the complete mitogenomes of two Characidae fish were determined and comparatively analyzed with those of nine other Characidae fish s...
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doaj-6631a958a616431da8d2b98c056a0a1f2021-02-01T06:08:22ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-02-011210.3389/fgene.2021.627402627402Mitochondrial Genome Structures and Phylogenetic Analyses of Two Tropical Characidae FishesCheng-He Sun0Cheng-He Sun1Hong-Yi Liu2Nan Xu3Xiao-Li Zhang4Qun Zhang5Bo-Ping Han6Department of Ecology, Jinan University, Guangzhou, ChinaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing, ChinaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing, ChinaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing, ChinaDepartment of Ecology, Jinan University, Guangzhou, ChinaDepartment of Ecology, Jinan University, Guangzhou, ChinaDepartment of Ecology, Jinan University, Guangzhou, ChinaThe Characidae family contains the largest number of tropical fish species. Morphological similarities make species identification difficult within this family. Here, the complete mitogenomes of two Characidae fish were determined and comparatively analyzed with those of nine other Characidae fish species. The two newly sequenced complete mitogenomes are circular DNA molecules with sizes of 16,701 bp (Hyphessobrycon amandae; MT484069) and 16,710 bp (Hemigrammus erythrozonus; MT484070); both have a highly conserved structure typical of Characidae, with the start codon ATN (ATG/ATT) and stop codon TAR (TAA/TAG) or an incomplete T−−/TA−. Most protein-coding genes of the 11 Characidae mitogenomes showed significant codon usage bias, and the protein-coding gene cox1 was found to be a comparatively slow-evolving gene. Phylogenetic analyses via the maximum likelihood and Bayesian inference methods confirmed that H. amandae and H. erythrozonus belong to the family Characidae. In all Characidae species studied, one genus was well supported; whereas other two genera showed marked differentiation. These findings provide a phylogenetic basis for improved classification of the family Characidae. Determining the mitogenomes of H. erythrozonus and H. amandae improves our understanding of the phylogeny and evolution of fish species.https://www.frontiersin.org/articles/10.3389/fgene.2021.627402/fullCharacidaeHemigrammus erythrozonusHyphessobrycon amandaemitochondrial genomephylogeny |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cheng-He Sun Cheng-He Sun Hong-Yi Liu Nan Xu Xiao-Li Zhang Qun Zhang Bo-Ping Han |
spellingShingle |
Cheng-He Sun Cheng-He Sun Hong-Yi Liu Nan Xu Xiao-Li Zhang Qun Zhang Bo-Ping Han Mitochondrial Genome Structures and Phylogenetic Analyses of Two Tropical Characidae Fishes Frontiers in Genetics Characidae Hemigrammus erythrozonus Hyphessobrycon amandae mitochondrial genome phylogeny |
author_facet |
Cheng-He Sun Cheng-He Sun Hong-Yi Liu Nan Xu Xiao-Li Zhang Qun Zhang Bo-Ping Han |
author_sort |
Cheng-He Sun |
title |
Mitochondrial Genome Structures and Phylogenetic Analyses of Two Tropical Characidae Fishes |
title_short |
Mitochondrial Genome Structures and Phylogenetic Analyses of Two Tropical Characidae Fishes |
title_full |
Mitochondrial Genome Structures and Phylogenetic Analyses of Two Tropical Characidae Fishes |
title_fullStr |
Mitochondrial Genome Structures and Phylogenetic Analyses of Two Tropical Characidae Fishes |
title_full_unstemmed |
Mitochondrial Genome Structures and Phylogenetic Analyses of Two Tropical Characidae Fishes |
title_sort |
mitochondrial genome structures and phylogenetic analyses of two tropical characidae fishes |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Genetics |
issn |
1664-8021 |
publishDate |
2021-02-01 |
description |
The Characidae family contains the largest number of tropical fish species. Morphological similarities make species identification difficult within this family. Here, the complete mitogenomes of two Characidae fish were determined and comparatively analyzed with those of nine other Characidae fish species. The two newly sequenced complete mitogenomes are circular DNA molecules with sizes of 16,701 bp (Hyphessobrycon amandae; MT484069) and 16,710 bp (Hemigrammus erythrozonus; MT484070); both have a highly conserved structure typical of Characidae, with the start codon ATN (ATG/ATT) and stop codon TAR (TAA/TAG) or an incomplete T−−/TA−. Most protein-coding genes of the 11 Characidae mitogenomes showed significant codon usage bias, and the protein-coding gene cox1 was found to be a comparatively slow-evolving gene. Phylogenetic analyses via the maximum likelihood and Bayesian inference methods confirmed that H. amandae and H. erythrozonus belong to the family Characidae. In all Characidae species studied, one genus was well supported; whereas other two genera showed marked differentiation. These findings provide a phylogenetic basis for improved classification of the family Characidae. Determining the mitogenomes of H. erythrozonus and H. amandae improves our understanding of the phylogeny and evolution of fish species. |
topic |
Characidae Hemigrammus erythrozonus Hyphessobrycon amandae mitochondrial genome phylogeny |
url |
https://www.frontiersin.org/articles/10.3389/fgene.2021.627402/full |
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