Advances in genome-wide RNAi cellular screens: a case study using the <it>Drosophila</it> JAK/STAT pathway
<p>Abstract</p> <p>Background</p> <p>Genome-scale RNA-interference (RNAi) screens are becoming ever more common gene discovery tools. However, whilst every screen identifies interacting genes, less attention has been given to how factors such as library design and post-...
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doaj-663ac7c5067248ab856dc9d91b55dbe82020-11-24T20:49:50ZengBMCBMC Genomics1471-21642012-09-0113150610.1186/1471-2164-13-506Advances in genome-wide RNAi cellular screens: a case study using the <it>Drosophila</it> JAK/STAT pathwayFisher Katherine HWright Victoria MTaylor AmyZeidler Martin PBrown Stephen<p>Abstract</p> <p>Background</p> <p>Genome-scale RNA-interference (RNAi) screens are becoming ever more common gene discovery tools. However, whilst every screen identifies interacting genes, less attention has been given to how factors such as library design and post-screening bioinformatics may be effecting the data generated.</p> <p>Results</p> <p>Here we present a new genome-wide RNAi screen of the <it>Drosophila</it> JAK/STAT signalling pathway undertaken in the Sheffield RNAi Screening Facility (SRSF). This screen was carried out using a second-generation, computationally optimised dsRNA library and analysed using current methods and bioinformatic tools. To examine advances in RNAi screening technology, we compare this screen to a biologically very similar screen undertaken in 2005 with a first-generation library. Both screens used the same cell line, reporters and experimental design, with the SRSF screen identifying 42 putative regulators of JAK/STAT signalling, 22 of which verified in a secondary screen and 16 verified with an independent probe design. Following reanalysis of the original screen data, comparisons of the two gene lists allows us to make estimates of false discovery rates in the SRSF data and to conduct an assessment of off-target effects (OTEs) associated with both libraries. We discuss the differences and similarities between the resulting data sets and examine the relative improvements in gene discovery protocols.</p> <p>Conclusions</p> <p>Our work represents one of the first direct comparisons between first- and second-generation libraries and shows that modern library designs together with methodological advances have had a significant influence on genome-scale RNAi screens.</p> http://www.biomedcentral.com/1471-2164/13/506Genome screeningRNAiOff-target effectJAK/STAT pathwayFunctional genomicsdsRNA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fisher Katherine H Wright Victoria M Taylor Amy Zeidler Martin P Brown Stephen |
spellingShingle |
Fisher Katherine H Wright Victoria M Taylor Amy Zeidler Martin P Brown Stephen Advances in genome-wide RNAi cellular screens: a case study using the <it>Drosophila</it> JAK/STAT pathway BMC Genomics Genome screening RNAi Off-target effect JAK/STAT pathway Functional genomics dsRNA |
author_facet |
Fisher Katherine H Wright Victoria M Taylor Amy Zeidler Martin P Brown Stephen |
author_sort |
Fisher Katherine H |
title |
Advances in genome-wide RNAi cellular screens: a case study using the <it>Drosophila</it> JAK/STAT pathway |
title_short |
Advances in genome-wide RNAi cellular screens: a case study using the <it>Drosophila</it> JAK/STAT pathway |
title_full |
Advances in genome-wide RNAi cellular screens: a case study using the <it>Drosophila</it> JAK/STAT pathway |
title_fullStr |
Advances in genome-wide RNAi cellular screens: a case study using the <it>Drosophila</it> JAK/STAT pathway |
title_full_unstemmed |
Advances in genome-wide RNAi cellular screens: a case study using the <it>Drosophila</it> JAK/STAT pathway |
title_sort |
advances in genome-wide rnai cellular screens: a case study using the <it>drosophila</it> jak/stat pathway |
publisher |
BMC |
series |
BMC Genomics |
issn |
1471-2164 |
publishDate |
2012-09-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Genome-scale RNA-interference (RNAi) screens are becoming ever more common gene discovery tools. However, whilst every screen identifies interacting genes, less attention has been given to how factors such as library design and post-screening bioinformatics may be effecting the data generated.</p> <p>Results</p> <p>Here we present a new genome-wide RNAi screen of the <it>Drosophila</it> JAK/STAT signalling pathway undertaken in the Sheffield RNAi Screening Facility (SRSF). This screen was carried out using a second-generation, computationally optimised dsRNA library and analysed using current methods and bioinformatic tools. To examine advances in RNAi screening technology, we compare this screen to a biologically very similar screen undertaken in 2005 with a first-generation library. Both screens used the same cell line, reporters and experimental design, with the SRSF screen identifying 42 putative regulators of JAK/STAT signalling, 22 of which verified in a secondary screen and 16 verified with an independent probe design. Following reanalysis of the original screen data, comparisons of the two gene lists allows us to make estimates of false discovery rates in the SRSF data and to conduct an assessment of off-target effects (OTEs) associated with both libraries. We discuss the differences and similarities between the resulting data sets and examine the relative improvements in gene discovery protocols.</p> <p>Conclusions</p> <p>Our work represents one of the first direct comparisons between first- and second-generation libraries and shows that modern library designs together with methodological advances have had a significant influence on genome-scale RNAi screens.</p> |
topic |
Genome screening RNAi Off-target effect JAK/STAT pathway Functional genomics dsRNA |
url |
http://www.biomedcentral.com/1471-2164/13/506 |
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