Early growth stages salinity stress tolerance in CM72 x Gairdner doubled haploid barley population.
A doubled haploid (DH) population of barley (Hordeum vulgare L.) generated from salinity tolerant genotype CM72 and salinity sensitive variety Gairdner was studied for salinity stress tolerance at germination, seedling emergence and first leaf full expansion growth stages. Germination study was cond...
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doaj-6650c5de3af348039d562c7a64367bdd2020-11-24T22:11:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01126e017971510.1371/journal.pone.0179715Early growth stages salinity stress tolerance in CM72 x Gairdner doubled haploid barley population.Tefera Tolera AngessaXiao-Qi ZhangGaofeng ZhouSue BroughtonWenying ZhangChengdao LiA doubled haploid (DH) population of barley (Hordeum vulgare L.) generated from salinity tolerant genotype CM72 and salinity sensitive variety Gairdner was studied for salinity stress tolerance at germination, seedling emergence and first leaf full expansion growth stages. Germination study was conducted with deionized water, 150 mM and 300 mM NaCl treatments. Seedling stage salinity tolerance was conducted with three treatments: control, 150 mM NaCl added at seedling emergence and first leaf full expansion growth stages. Results from this study revealed transgressive phenotypic segregations for germination percentage and biomass at seedling stage. Twelve QTL were identified on chromosomes 2H-6H each explaining 10-25% of the phenotypic variations. A QTL located at 176.5 cM on chromosome 3H was linked with fresh weight per plant and dry weight per plant in salinity stress induced at first leaf full expansion growth stage, and dry weight per plant in salinity stress induced at seedling emergence. A stable QTL for germination at both 150 and 300 mM salinity stress was mapped on chromosome 2H but distantly located from a QTL linked with seedling stage salinity stress tolerance. QTL, associated markers and genotypes identified in this study play important roles in developing salinity stress tolerant barley varieties.http://europepmc.org/articles/PMC5480976?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tefera Tolera Angessa Xiao-Qi Zhang Gaofeng Zhou Sue Broughton Wenying Zhang Chengdao Li |
spellingShingle |
Tefera Tolera Angessa Xiao-Qi Zhang Gaofeng Zhou Sue Broughton Wenying Zhang Chengdao Li Early growth stages salinity stress tolerance in CM72 x Gairdner doubled haploid barley population. PLoS ONE |
author_facet |
Tefera Tolera Angessa Xiao-Qi Zhang Gaofeng Zhou Sue Broughton Wenying Zhang Chengdao Li |
author_sort |
Tefera Tolera Angessa |
title |
Early growth stages salinity stress tolerance in CM72 x Gairdner doubled haploid barley population. |
title_short |
Early growth stages salinity stress tolerance in CM72 x Gairdner doubled haploid barley population. |
title_full |
Early growth stages salinity stress tolerance in CM72 x Gairdner doubled haploid barley population. |
title_fullStr |
Early growth stages salinity stress tolerance in CM72 x Gairdner doubled haploid barley population. |
title_full_unstemmed |
Early growth stages salinity stress tolerance in CM72 x Gairdner doubled haploid barley population. |
title_sort |
early growth stages salinity stress tolerance in cm72 x gairdner doubled haploid barley population. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
A doubled haploid (DH) population of barley (Hordeum vulgare L.) generated from salinity tolerant genotype CM72 and salinity sensitive variety Gairdner was studied for salinity stress tolerance at germination, seedling emergence and first leaf full expansion growth stages. Germination study was conducted with deionized water, 150 mM and 300 mM NaCl treatments. Seedling stage salinity tolerance was conducted with three treatments: control, 150 mM NaCl added at seedling emergence and first leaf full expansion growth stages. Results from this study revealed transgressive phenotypic segregations for germination percentage and biomass at seedling stage. Twelve QTL were identified on chromosomes 2H-6H each explaining 10-25% of the phenotypic variations. A QTL located at 176.5 cM on chromosome 3H was linked with fresh weight per plant and dry weight per plant in salinity stress induced at first leaf full expansion growth stage, and dry weight per plant in salinity stress induced at seedling emergence. A stable QTL for germination at both 150 and 300 mM salinity stress was mapped on chromosome 2H but distantly located from a QTL linked with seedling stage salinity stress tolerance. QTL, associated markers and genotypes identified in this study play important roles in developing salinity stress tolerant barley varieties. |
url |
http://europepmc.org/articles/PMC5480976?pdf=render |
work_keys_str_mv |
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