Fail-Safe System against Potential Tumorigenicity after Transplantation of iPSC Derivatives
Human induced pluripotent stem cells (iPSCs) are promising in regenerative medicine. However, the risks of teratoma formation and the overgrowth of the transplanted cells continue to be major hurdles that must be overcome. Here, we examined the efficacy of the inducible caspase-9 (iCaspase9) gene as...
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doaj-668d828c99c0461a96ad761bc1e5fc402020-11-24T21:23:44ZengElsevierStem Cell Reports2213-67112017-03-018367368410.1016/j.stemcr.2017.02.003Fail-Safe System against Potential Tumorigenicity after Transplantation of iPSC DerivativesGo Itakura0Soya Kawabata1Miki Ando2Yuichiro Nishiyama3Keiko Sugai4Masahiro Ozaki5Tsuyoshi Iida6Toshiki Ookubo7Kota Kojima8Rei Kashiwagi9Kaori Yasutake10Hiromitsu Nakauchi11Hiroyuki Miyoshi12Narihito Nagoshi13Jun Kohyama14Akio Iwanami15Morio Matsumoto16Masaya Nakamura17Hideyuki Okano18Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanDepartment of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanDivision of Stem Cell Therapy, Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, JapanDepartment of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanDepartment of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanDepartment of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanDepartment of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanDepartment of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanDepartment of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanDepartment of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanDepartment of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanDivision of Stem Cell Therapy, Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, JapanDepartment of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanDepartment of Orthopaedic Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanDepartment of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanDepartment of Orthopaedic Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanDepartment of Orthopaedic Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanDepartment of Orthopaedic Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanDepartment of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanHuman induced pluripotent stem cells (iPSCs) are promising in regenerative medicine. However, the risks of teratoma formation and the overgrowth of the transplanted cells continue to be major hurdles that must be overcome. Here, we examined the efficacy of the inducible caspase-9 (iCaspase9) gene as a fail-safe against undesired tumorigenic transformation of iPSC-derived somatic cells. We used a lentiviral vector to transduce iCaspase9 into two iPSC lines and assessed its efficacy in vitro and in vivo. In vitro, the iCaspase9 system induced apoptosis in approximately 95% of both iPSCs and iPSC-derived neural stem/progenitor cells (iPSC-NS/PCs). To determine in vivo function, we transplanted iPSC-NS/PCs into the injured spinal cord of NOD/SCID mice. All transplanted cells whose mass effect was hindering motor function recovery were ablated upon transduction of iCaspase9. Our results suggest that the iCaspase9 system may serve as an important countermeasure against post-transplantation adverse events in stem cell transplant therapies.http://www.sciencedirect.com/science/article/pii/S2213671117300474induced pluripotent stem cells (iPSCs)iCaspase9iPSC-derived neural stem/progenitor cells (iPSC-NS/PCs)spinal cord injury |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Go Itakura Soya Kawabata Miki Ando Yuichiro Nishiyama Keiko Sugai Masahiro Ozaki Tsuyoshi Iida Toshiki Ookubo Kota Kojima Rei Kashiwagi Kaori Yasutake Hiromitsu Nakauchi Hiroyuki Miyoshi Narihito Nagoshi Jun Kohyama Akio Iwanami Morio Matsumoto Masaya Nakamura Hideyuki Okano |
spellingShingle |
Go Itakura Soya Kawabata Miki Ando Yuichiro Nishiyama Keiko Sugai Masahiro Ozaki Tsuyoshi Iida Toshiki Ookubo Kota Kojima Rei Kashiwagi Kaori Yasutake Hiromitsu Nakauchi Hiroyuki Miyoshi Narihito Nagoshi Jun Kohyama Akio Iwanami Morio Matsumoto Masaya Nakamura Hideyuki Okano Fail-Safe System against Potential Tumorigenicity after Transplantation of iPSC Derivatives Stem Cell Reports induced pluripotent stem cells (iPSCs) iCaspase9 iPSC-derived neural stem/progenitor cells (iPSC-NS/PCs) spinal cord injury |
author_facet |
Go Itakura Soya Kawabata Miki Ando Yuichiro Nishiyama Keiko Sugai Masahiro Ozaki Tsuyoshi Iida Toshiki Ookubo Kota Kojima Rei Kashiwagi Kaori Yasutake Hiromitsu Nakauchi Hiroyuki Miyoshi Narihito Nagoshi Jun Kohyama Akio Iwanami Morio Matsumoto Masaya Nakamura Hideyuki Okano |
author_sort |
Go Itakura |
title |
Fail-Safe System against Potential Tumorigenicity after Transplantation of iPSC Derivatives |
title_short |
Fail-Safe System against Potential Tumorigenicity after Transplantation of iPSC Derivatives |
title_full |
Fail-Safe System against Potential Tumorigenicity after Transplantation of iPSC Derivatives |
title_fullStr |
Fail-Safe System against Potential Tumorigenicity after Transplantation of iPSC Derivatives |
title_full_unstemmed |
Fail-Safe System against Potential Tumorigenicity after Transplantation of iPSC Derivatives |
title_sort |
fail-safe system against potential tumorigenicity after transplantation of ipsc derivatives |
publisher |
Elsevier |
series |
Stem Cell Reports |
issn |
2213-6711 |
publishDate |
2017-03-01 |
description |
Human induced pluripotent stem cells (iPSCs) are promising in regenerative medicine. However, the risks of teratoma formation and the overgrowth of the transplanted cells continue to be major hurdles that must be overcome. Here, we examined the efficacy of the inducible caspase-9 (iCaspase9) gene as a fail-safe against undesired tumorigenic transformation of iPSC-derived somatic cells. We used a lentiviral vector to transduce iCaspase9 into two iPSC lines and assessed its efficacy in vitro and in vivo. In vitro, the iCaspase9 system induced apoptosis in approximately 95% of both iPSCs and iPSC-derived neural stem/progenitor cells (iPSC-NS/PCs). To determine in vivo function, we transplanted iPSC-NS/PCs into the injured spinal cord of NOD/SCID mice. All transplanted cells whose mass effect was hindering motor function recovery were ablated upon transduction of iCaspase9. Our results suggest that the iCaspase9 system may serve as an important countermeasure against post-transplantation adverse events in stem cell transplant therapies. |
topic |
induced pluripotent stem cells (iPSCs) iCaspase9 iPSC-derived neural stem/progenitor cells (iPSC-NS/PCs) spinal cord injury |
url |
http://www.sciencedirect.com/science/article/pii/S2213671117300474 |
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