Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology

Two inorganic solids have been evaluated as supports of Lipozyme RM IM, a Rhizomucor miehei lipase immobilized on a macroporous anion exchange resin, in order to improve its application as a biocatalyst in the synthesis of biofuels. The experimental conditions have been optimized to get the selectiv...

Full description

Bibliographic Details
Main Authors: Juan Calero, Diego Luna, Carlos Luna, Felipa M. Bautista, Beatriz Hurtado, Antonio A. Romero, Alejandro Posadillo, Rafael Estevez
Format: Article
Language:English
Published: MDPI AG 2019-03-01
Series:Energies
Subjects:
Online Access:http://www.mdpi.com/1996-1073/12/5/831
id doaj-6690ca60428b4aa4b494e6b1d4fcb537
record_format Article
spelling doaj-6690ca60428b4aa4b494e6b1d4fcb5372020-11-24T21:20:53ZengMDPI AGEnergies1996-10732019-03-0112583110.3390/en12050831en12050831Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface MethodologyJuan Calero0Diego Luna1Carlos Luna2Felipa M. Bautista3Beatriz Hurtado4Antonio A. Romero5Alejandro Posadillo6Rafael Estevez7Departamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, SpainDepartamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, SpainDepartamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, SpainDepartamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, SpainDepartamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, SpainDepartamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, SpainSeneca Green Catalyst S.L., Campus de Rabanales, 14014 Córdoba, SpainDepartamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, SpainTwo inorganic solids have been evaluated as supports of Lipozyme RM IM, a Rhizomucor miehei lipase immobilized on a macroporous anion exchange resin, in order to improve its application as a biocatalyst in the synthesis of biofuels. The experimental conditions have been optimized to get the selective transesterification of sunflower oil, by using a multi-factorial design based on the response surface methodology (RSM). In this way, the effects of several reaction parameters on the selective ethanolysis of triglycerides to produce Ecodiesel, a biodiesel-like biofuel constitute by one mole of monoglyceride (MG) and two moles of fatty acid ethyl ester (FAEE), have been evaluated. Thus, it was obtained that a 6:1 oil/ethanol molar ratio, 0.215 g of biocatalyst supported in silica-gel (0.015 g Lipase/0.2 g silica-gel), 50 µL of 10 N NaOH, together with previous optimized reaction parameters, 35 °C reaction temperature and 120 min of reaction time, gave the best results (conversions around 70%; selectivity around 65%; kinematic viscosities about 9.3 mm2/s) in the reaction studied. Besides, Lipozyme RM IM, supported on silica-gel, biocatalyst exhibited a very good stability, remaining its activity even after 15 cycles.http://www.mdpi.com/1996-1073/12/5/831biodieselEcodieselselective ethanolysissunflower oilLipozyme RM IMRhizomucor mieheiANOVA methodresponse surface methodology
collection DOAJ
language English
format Article
sources DOAJ
author Juan Calero
Diego Luna
Carlos Luna
Felipa M. Bautista
Beatriz Hurtado
Antonio A. Romero
Alejandro Posadillo
Rafael Estevez
spellingShingle Juan Calero
Diego Luna
Carlos Luna
Felipa M. Bautista
Beatriz Hurtado
Antonio A. Romero
Alejandro Posadillo
Rafael Estevez
Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology
Energies
biodiesel
Ecodiesel
selective ethanolysis
sunflower oil
Lipozyme RM IM
Rhizomucor miehei
ANOVA method
response surface methodology
author_facet Juan Calero
Diego Luna
Carlos Luna
Felipa M. Bautista
Beatriz Hurtado
Antonio A. Romero
Alejandro Posadillo
Rafael Estevez
author_sort Juan Calero
title Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology
title_short Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology
title_full Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology
title_fullStr Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology
title_full_unstemmed Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology
title_sort rhizomucor miehei lipase supported on inorganic solids, as biocatalyst for the synthesis of biofuels: improving the experimental conditions by response surface methodology
publisher MDPI AG
series Energies
issn 1996-1073
publishDate 2019-03-01
description Two inorganic solids have been evaluated as supports of Lipozyme RM IM, a Rhizomucor miehei lipase immobilized on a macroporous anion exchange resin, in order to improve its application as a biocatalyst in the synthesis of biofuels. The experimental conditions have been optimized to get the selective transesterification of sunflower oil, by using a multi-factorial design based on the response surface methodology (RSM). In this way, the effects of several reaction parameters on the selective ethanolysis of triglycerides to produce Ecodiesel, a biodiesel-like biofuel constitute by one mole of monoglyceride (MG) and two moles of fatty acid ethyl ester (FAEE), have been evaluated. Thus, it was obtained that a 6:1 oil/ethanol molar ratio, 0.215 g of biocatalyst supported in silica-gel (0.015 g Lipase/0.2 g silica-gel), 50 µL of 10 N NaOH, together with previous optimized reaction parameters, 35 °C reaction temperature and 120 min of reaction time, gave the best results (conversions around 70%; selectivity around 65%; kinematic viscosities about 9.3 mm2/s) in the reaction studied. Besides, Lipozyme RM IM, supported on silica-gel, biocatalyst exhibited a very good stability, remaining its activity even after 15 cycles.
topic biodiesel
Ecodiesel
selective ethanolysis
sunflower oil
Lipozyme RM IM
Rhizomucor miehei
ANOVA method
response surface methodology
url http://www.mdpi.com/1996-1073/12/5/831
work_keys_str_mv AT juancalero rhizomucormieheilipasesupportedoninorganicsolidsasbiocatalystforthesynthesisofbiofuelsimprovingtheexperimentalconditionsbyresponsesurfacemethodology
AT diegoluna rhizomucormieheilipasesupportedoninorganicsolidsasbiocatalystforthesynthesisofbiofuelsimprovingtheexperimentalconditionsbyresponsesurfacemethodology
AT carlosluna rhizomucormieheilipasesupportedoninorganicsolidsasbiocatalystforthesynthesisofbiofuelsimprovingtheexperimentalconditionsbyresponsesurfacemethodology
AT felipambautista rhizomucormieheilipasesupportedoninorganicsolidsasbiocatalystforthesynthesisofbiofuelsimprovingtheexperimentalconditionsbyresponsesurfacemethodology
AT beatrizhurtado rhizomucormieheilipasesupportedoninorganicsolidsasbiocatalystforthesynthesisofbiofuelsimprovingtheexperimentalconditionsbyresponsesurfacemethodology
AT antonioaromero rhizomucormieheilipasesupportedoninorganicsolidsasbiocatalystforthesynthesisofbiofuelsimprovingtheexperimentalconditionsbyresponsesurfacemethodology
AT alejandroposadillo rhizomucormieheilipasesupportedoninorganicsolidsasbiocatalystforthesynthesisofbiofuelsimprovingtheexperimentalconditionsbyresponsesurfacemethodology
AT rafaelestevez rhizomucormieheilipasesupportedoninorganicsolidsasbiocatalystforthesynthesisofbiofuelsimprovingtheexperimentalconditionsbyresponsesurfacemethodology
_version_ 1726002374138396672