Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology
Two inorganic solids have been evaluated as supports of Lipozyme RM IM, a Rhizomucor miehei lipase immobilized on a macroporous anion exchange resin, in order to improve its application as a biocatalyst in the synthesis of biofuels. The experimental conditions have been optimized to get the selectiv...
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doaj-6690ca60428b4aa4b494e6b1d4fcb5372020-11-24T21:20:53ZengMDPI AGEnergies1996-10732019-03-0112583110.3390/en12050831en12050831Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface MethodologyJuan Calero0Diego Luna1Carlos Luna2Felipa M. Bautista3Beatriz Hurtado4Antonio A. Romero5Alejandro Posadillo6Rafael Estevez7Departamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, SpainDepartamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, SpainDepartamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, SpainDepartamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, SpainDepartamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, SpainDepartamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, SpainSeneca Green Catalyst S.L., Campus de Rabanales, 14014 Córdoba, SpainDepartamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, SpainTwo inorganic solids have been evaluated as supports of Lipozyme RM IM, a Rhizomucor miehei lipase immobilized on a macroporous anion exchange resin, in order to improve its application as a biocatalyst in the synthesis of biofuels. The experimental conditions have been optimized to get the selective transesterification of sunflower oil, by using a multi-factorial design based on the response surface methodology (RSM). In this way, the effects of several reaction parameters on the selective ethanolysis of triglycerides to produce Ecodiesel, a biodiesel-like biofuel constitute by one mole of monoglyceride (MG) and two moles of fatty acid ethyl ester (FAEE), have been evaluated. Thus, it was obtained that a 6:1 oil/ethanol molar ratio, 0.215 g of biocatalyst supported in silica-gel (0.015 g Lipase/0.2 g silica-gel), 50 µL of 10 N NaOH, together with previous optimized reaction parameters, 35 °C reaction temperature and 120 min of reaction time, gave the best results (conversions around 70%; selectivity around 65%; kinematic viscosities about 9.3 mm2/s) in the reaction studied. Besides, Lipozyme RM IM, supported on silica-gel, biocatalyst exhibited a very good stability, remaining its activity even after 15 cycles.http://www.mdpi.com/1996-1073/12/5/831biodieselEcodieselselective ethanolysissunflower oilLipozyme RM IMRhizomucor mieheiANOVA methodresponse surface methodology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juan Calero Diego Luna Carlos Luna Felipa M. Bautista Beatriz Hurtado Antonio A. Romero Alejandro Posadillo Rafael Estevez |
spellingShingle |
Juan Calero Diego Luna Carlos Luna Felipa M. Bautista Beatriz Hurtado Antonio A. Romero Alejandro Posadillo Rafael Estevez Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology Energies biodiesel Ecodiesel selective ethanolysis sunflower oil Lipozyme RM IM Rhizomucor miehei ANOVA method response surface methodology |
author_facet |
Juan Calero Diego Luna Carlos Luna Felipa M. Bautista Beatriz Hurtado Antonio A. Romero Alejandro Posadillo Rafael Estevez |
author_sort |
Juan Calero |
title |
Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology |
title_short |
Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology |
title_full |
Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology |
title_fullStr |
Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology |
title_full_unstemmed |
Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology |
title_sort |
rhizomucor miehei lipase supported on inorganic solids, as biocatalyst for the synthesis of biofuels: improving the experimental conditions by response surface methodology |
publisher |
MDPI AG |
series |
Energies |
issn |
1996-1073 |
publishDate |
2019-03-01 |
description |
Two inorganic solids have been evaluated as supports of Lipozyme RM IM, a Rhizomucor miehei lipase immobilized on a macroporous anion exchange resin, in order to improve its application as a biocatalyst in the synthesis of biofuels. The experimental conditions have been optimized to get the selective transesterification of sunflower oil, by using a multi-factorial design based on the response surface methodology (RSM). In this way, the effects of several reaction parameters on the selective ethanolysis of triglycerides to produce Ecodiesel, a biodiesel-like biofuel constitute by one mole of monoglyceride (MG) and two moles of fatty acid ethyl ester (FAEE), have been evaluated. Thus, it was obtained that a 6:1 oil/ethanol molar ratio, 0.215 g of biocatalyst supported in silica-gel (0.015 g Lipase/0.2 g silica-gel), 50 µL of 10 N NaOH, together with previous optimized reaction parameters, 35 °C reaction temperature and 120 min of reaction time, gave the best results (conversions around 70%; selectivity around 65%; kinematic viscosities about 9.3 mm2/s) in the reaction studied. Besides, Lipozyme RM IM, supported on silica-gel, biocatalyst exhibited a very good stability, remaining its activity even after 15 cycles. |
topic |
biodiesel Ecodiesel selective ethanolysis sunflower oil Lipozyme RM IM Rhizomucor miehei ANOVA method response surface methodology |
url |
http://www.mdpi.com/1996-1073/12/5/831 |
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