Fibromodulin deficiency reduces collagen structural network but not glycosaminoglycan content in a syngeneic model of colon carcinoma.
Tumor barrier function in carcinoma represents a major challenge to treatment and is therefore an attractive target for increasing drug delivery. Variables related to tumor barrier include aberrant blood vessels, high interstitial fluid pressure, and the composition and structure of the extracellula...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2017-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC5565175?pdf=render |
id |
doaj-6696d8ce8d19410591ca54798fe5cdaa |
---|---|
record_format |
Article |
spelling |
doaj-6696d8ce8d19410591ca54798fe5cdaa2020-11-24T20:50:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018297310.1371/journal.pone.0182973Fibromodulin deficiency reduces collagen structural network but not glycosaminoglycan content in a syngeneic model of colon carcinoma.P Olof OlssonSebastian KalamajskiMarco MaccaranaÅke OldbergKristofer RubinTumor barrier function in carcinoma represents a major challenge to treatment and is therefore an attractive target for increasing drug delivery. Variables related to tumor barrier include aberrant blood vessels, high interstitial fluid pressure, and the composition and structure of the extracellular matrix. One of the proteins associated with dense extracellular matrices is fibromodulin, a collagen fibrillogenesis modulator expressed in tumor stroma but scarce in normal loose connective tissues. Here, we investigated the effects of fibromodulin on stroma ECM in a syngeneic murine colon carcinoma model. We show that fibromodulin deficiency decreased collagen fibril thickness but glycosaminoglycan content and composition were unchanged. Furthermore, vascular density, pericyte coverage and macrophage amount were unaffected. Fibromodulin can therefore be a unique effector of dense collagen matrix assembly in tumor stroma and, without affecting other major matrix components or the cellular composition, can function as a main agent in tumor barrier function.http://europepmc.org/articles/PMC5565175?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
P Olof Olsson Sebastian Kalamajski Marco Maccarana Åke Oldberg Kristofer Rubin |
spellingShingle |
P Olof Olsson Sebastian Kalamajski Marco Maccarana Åke Oldberg Kristofer Rubin Fibromodulin deficiency reduces collagen structural network but not glycosaminoglycan content in a syngeneic model of colon carcinoma. PLoS ONE |
author_facet |
P Olof Olsson Sebastian Kalamajski Marco Maccarana Åke Oldberg Kristofer Rubin |
author_sort |
P Olof Olsson |
title |
Fibromodulin deficiency reduces collagen structural network but not glycosaminoglycan content in a syngeneic model of colon carcinoma. |
title_short |
Fibromodulin deficiency reduces collagen structural network but not glycosaminoglycan content in a syngeneic model of colon carcinoma. |
title_full |
Fibromodulin deficiency reduces collagen structural network but not glycosaminoglycan content in a syngeneic model of colon carcinoma. |
title_fullStr |
Fibromodulin deficiency reduces collagen structural network but not glycosaminoglycan content in a syngeneic model of colon carcinoma. |
title_full_unstemmed |
Fibromodulin deficiency reduces collagen structural network but not glycosaminoglycan content in a syngeneic model of colon carcinoma. |
title_sort |
fibromodulin deficiency reduces collagen structural network but not glycosaminoglycan content in a syngeneic model of colon carcinoma. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
Tumor barrier function in carcinoma represents a major challenge to treatment and is therefore an attractive target for increasing drug delivery. Variables related to tumor barrier include aberrant blood vessels, high interstitial fluid pressure, and the composition and structure of the extracellular matrix. One of the proteins associated with dense extracellular matrices is fibromodulin, a collagen fibrillogenesis modulator expressed in tumor stroma but scarce in normal loose connective tissues. Here, we investigated the effects of fibromodulin on stroma ECM in a syngeneic murine colon carcinoma model. We show that fibromodulin deficiency decreased collagen fibril thickness but glycosaminoglycan content and composition were unchanged. Furthermore, vascular density, pericyte coverage and macrophage amount were unaffected. Fibromodulin can therefore be a unique effector of dense collagen matrix assembly in tumor stroma and, without affecting other major matrix components or the cellular composition, can function as a main agent in tumor barrier function. |
url |
http://europepmc.org/articles/PMC5565175?pdf=render |
work_keys_str_mv |
AT polofolsson fibromodulindeficiencyreducescollagenstructuralnetworkbutnotglycosaminoglycancontentinasyngeneicmodelofcoloncarcinoma AT sebastiankalamajski fibromodulindeficiencyreducescollagenstructuralnetworkbutnotglycosaminoglycancontentinasyngeneicmodelofcoloncarcinoma AT marcomaccarana fibromodulindeficiencyreducescollagenstructuralnetworkbutnotglycosaminoglycancontentinasyngeneicmodelofcoloncarcinoma AT akeoldberg fibromodulindeficiencyreducescollagenstructuralnetworkbutnotglycosaminoglycancontentinasyngeneicmodelofcoloncarcinoma AT kristoferrubin fibromodulindeficiencyreducescollagenstructuralnetworkbutnotglycosaminoglycancontentinasyngeneicmodelofcoloncarcinoma |
_version_ |
1716804207630090240 |