Update and New Epidemiological Aspects about Grapevine Yellows in Chile
To date, phytoplasmas belonging to six ribosomal subgroups have been detected to infect grapevines in Chile in 36 percent of the sampled plants. A new survey on the presence of grapevine yellows was carried out from 2016 to 2020, and 330 grapevine plants from the most important wine regions of the c...
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doaj-66987ca1ef6240c6859b520a375464212020-11-25T04:03:51ZengMDPI AGPathogens2076-08172020-11-01993393310.3390/pathogens9110933Update and New Epidemiological Aspects about Grapevine Yellows in ChileNicolás Quiroga0Camila Gamboa1Daniela Soto2Ana Maria Pino3Alan Zamorano4Juan Campodonico5Alberto Alma6Assunta Bertaccini7Nicola Fiore8Departamento de Sanidad Vegetal, Facultad de Ciencias Agronómicas, Universidad de Chile, La Pintana, 8820808 Santiago, ChileDepartamento de Sanidad Vegetal, Facultad de Ciencias Agronómicas, Universidad de Chile, La Pintana, 8820808 Santiago, ChileDepartamento de Sanidad Vegetal, Facultad de Ciencias Agronómicas, Universidad de Chile, La Pintana, 8820808 Santiago, ChileDepartamento de Sanidad Vegetal, Facultad de Ciencias Agronómicas, Universidad de Chile, La Pintana, 8820808 Santiago, ChileDepartamento de Sanidad Vegetal, Facultad de Ciencias Agronómicas, Universidad de Chile, La Pintana, 8820808 Santiago, ChilePh.D. Program in Science, Ecology and Evolution mention, Facultad de Ciencias, Universidad Austral de Chile, 5091000 Valdivia, ChileForest and Food Sciences and Technologies, Department of Agricultural, Università di Torino, DISAFA, 10095 Grugliasco (TO), ItalyAlma Mater Studiorum, Department of Agricultural and Food Sciences, Università di Bologna, 40127 Bologna, ItalyDepartamento de Sanidad Vegetal, Facultad de Ciencias Agronómicas, Universidad de Chile, La Pintana, 8820808 Santiago, ChileTo date, phytoplasmas belonging to six ribosomal subgroups have been detected to infect grapevines in Chile in 36 percent of the sampled plants. A new survey on the presence of grapevine yellows was carried out from 2016 to 2020, and 330 grapevine plants from the most important wine regions of the country were sampled and analyzed by nested PCR/RFLP analyses. Phytoplasmas enclosed in subgroups 16SrIII-J and 16SrVII-A were identified with infection rates of 17% and 2%, respectively. The vineyards in which the phytoplasma-infected plants were detected were further inspected to identify alternative host plants and insects of potential epidemiological relevance. Five previously unreported plant species resulted positive for 16SrIII-J phytoplasma (<i>Rosa</i> spp., <i>Brassica rapa</i>, <i>Erodium</i> spp., <i>Malva</i> spp. and <i>Rubus ulmifolius</i>) and five insect species were fully or partially identified (<i>Amplicephalus ornatus</i>, <i>A. pallidus</i>, <i>A. curtulus</i>, <i>Bergallia</i> sp., <i>Exitianus obscurinervis</i>) as potential vectors of 16SrIII-J phytoplasmas. The 16SrVII-A phytoplasmas were not detected in non-grape plant species nor in insects. This work establishes updated guidelines for the study, management, and prevention of grapevine yellows in Chile, and in other grapevine growing regions of South America.https://www.mdpi.com/2076-0817/9/11/933insects vectornested-PCR/RFLPsequencingweeds |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nicolás Quiroga Camila Gamboa Daniela Soto Ana Maria Pino Alan Zamorano Juan Campodonico Alberto Alma Assunta Bertaccini Nicola Fiore |
spellingShingle |
Nicolás Quiroga Camila Gamboa Daniela Soto Ana Maria Pino Alan Zamorano Juan Campodonico Alberto Alma Assunta Bertaccini Nicola Fiore Update and New Epidemiological Aspects about Grapevine Yellows in Chile Pathogens insects vector nested-PCR/RFLP sequencing weeds |
author_facet |
Nicolás Quiroga Camila Gamboa Daniela Soto Ana Maria Pino Alan Zamorano Juan Campodonico Alberto Alma Assunta Bertaccini Nicola Fiore |
author_sort |
Nicolás Quiroga |
title |
Update and New Epidemiological Aspects about Grapevine Yellows in Chile |
title_short |
Update and New Epidemiological Aspects about Grapevine Yellows in Chile |
title_full |
Update and New Epidemiological Aspects about Grapevine Yellows in Chile |
title_fullStr |
Update and New Epidemiological Aspects about Grapevine Yellows in Chile |
title_full_unstemmed |
Update and New Epidemiological Aspects about Grapevine Yellows in Chile |
title_sort |
update and new epidemiological aspects about grapevine yellows in chile |
publisher |
MDPI AG |
series |
Pathogens |
issn |
2076-0817 |
publishDate |
2020-11-01 |
description |
To date, phytoplasmas belonging to six ribosomal subgroups have been detected to infect grapevines in Chile in 36 percent of the sampled plants. A new survey on the presence of grapevine yellows was carried out from 2016 to 2020, and 330 grapevine plants from the most important wine regions of the country were sampled and analyzed by nested PCR/RFLP analyses. Phytoplasmas enclosed in subgroups 16SrIII-J and 16SrVII-A were identified with infection rates of 17% and 2%, respectively. The vineyards in which the phytoplasma-infected plants were detected were further inspected to identify alternative host plants and insects of potential epidemiological relevance. Five previously unreported plant species resulted positive for 16SrIII-J phytoplasma (<i>Rosa</i> spp., <i>Brassica rapa</i>, <i>Erodium</i> spp., <i>Malva</i> spp. and <i>Rubus ulmifolius</i>) and five insect species were fully or partially identified (<i>Amplicephalus ornatus</i>, <i>A. pallidus</i>, <i>A. curtulus</i>, <i>Bergallia</i> sp., <i>Exitianus obscurinervis</i>) as potential vectors of 16SrIII-J phytoplasmas. The 16SrVII-A phytoplasmas were not detected in non-grape plant species nor in insects. This work establishes updated guidelines for the study, management, and prevention of grapevine yellows in Chile, and in other grapevine growing regions of South America. |
topic |
insects vector nested-PCR/RFLP sequencing weeds |
url |
https://www.mdpi.com/2076-0817/9/11/933 |
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