Biocompatibility of Mineral Trioxide Aggregate Mixed with Different Accelerators: an Animal Study
Statement of the Problem: Several additives have been introduced to decrease the setting time of MTA (mineral trioxide aggregate). For clinical applications, it is essential to investigate the biocompatibility of these materials. Purpose: The present study evaluated the tissue response to MTA that...
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Shiraz University of Medical Sciences
2020-03-01
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doaj-66ac6f3f01224f6a8d0cc43df5ef73042020-11-25T01:27:50ZengShiraz University of Medical SciencesJournal of Dentistry2345-64852345-64182020-03-012114855Biocompatibility of Mineral Trioxide Aggregate Mixed with Different Accelerators: an Animal StudyMitra Tabari0Maryam Seyed Majidi1Mahtab Hamzeh2Saeideh Ghoreishi3Dental Material Research Center, Dept. of Pediatric Dentistry, Babol University of Medical Sciences, Babol, IranDept. of Oral and Maxillofacial Pathology, Babol University of Medical Sciences, Babol, IranOral Health Research Center, Dept. of Pediatric Dentistry, Babol University of Medical Sciences, Babol, IranDental Material Research Center, Dept. of Pediatric Dentistry, Dental Research Institute, Isfahan University of Medical Sciences, Isfahan, IranStatement of the Problem: Several additives have been introduced to decrease the setting time of MTA (mineral trioxide aggregate). For clinical applications, it is essential to investigate the biocompatibility of these materials. Purpose: The present study evaluated the tissue response to MTA that has been separately mixed with citric acid, calcium lactate gluconate (CLG), and Na2HPo4. Materials and Method: In this experimental study Twenty one Wistar rats were divided into three groups of 7, 14 and 30 days follow up periods. Sterile polyethylene tubes were subsequently filled with MTA separately mixed with distilled water, 0.1% citric acid, 0.43% calcium lactate gluconate (CLG) and 15% Na2HPO4 and afterwards implanted subcutaneously. Empty tubes were implanted as negative control. At the end of their respective periods, the animals were sacrificed by anesthetic overdose and a biopsy was performed. The inflammatory responses were scored, classified and statistically analyzed using Kruskal-Wallis and Man-Whitney tests. Statistical significance was defined as p< 0.05. Results: There was no significant difference between test groups in any time period after implantation but the mean values of inflammatory responses were significantly more than that of the negative control group (p> 0.05). The mean values of inflammatory responses were decreasing over time in all test groups. These values did not significantly differ in any group except the CLG and Na2HPO4 groups. Conclusion: The inflammatory responses induced by MTA mixed with citric acid and MTA mixed with Na2HPo4 were comparable to that of the control MTA. MTA mixed with CLG provoked a moderate-to-severe inflammatory response at 7 days after implantation, so further study is required before clinical application of this cement.http://dentjods.sums.ac.ir/article_45894.htmlbiocompatibility ; mineral trioxide aggregate ; accelerator |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mitra Tabari Maryam Seyed Majidi Mahtab Hamzeh Saeideh Ghoreishi |
spellingShingle |
Mitra Tabari Maryam Seyed Majidi Mahtab Hamzeh Saeideh Ghoreishi Biocompatibility of Mineral Trioxide Aggregate Mixed with Different Accelerators: an Animal Study Journal of Dentistry biocompatibility ; mineral trioxide aggregate ; accelerator |
author_facet |
Mitra Tabari Maryam Seyed Majidi Mahtab Hamzeh Saeideh Ghoreishi |
author_sort |
Mitra Tabari |
title |
Biocompatibility of Mineral Trioxide Aggregate Mixed with Different Accelerators: an Animal Study |
title_short |
Biocompatibility of Mineral Trioxide Aggregate Mixed with Different Accelerators: an Animal Study |
title_full |
Biocompatibility of Mineral Trioxide Aggregate Mixed with Different Accelerators: an Animal Study |
title_fullStr |
Biocompatibility of Mineral Trioxide Aggregate Mixed with Different Accelerators: an Animal Study |
title_full_unstemmed |
Biocompatibility of Mineral Trioxide Aggregate Mixed with Different Accelerators: an Animal Study |
title_sort |
biocompatibility of mineral trioxide aggregate mixed with different accelerators: an animal study |
publisher |
Shiraz University of Medical Sciences |
series |
Journal of Dentistry |
issn |
2345-6485 2345-6418 |
publishDate |
2020-03-01 |
description |
Statement of the Problem: Several additives have been introduced to decrease the setting time of MTA (mineral trioxide aggregate). For clinical applications, it is essential to investigate the biocompatibility of these materials.
Purpose: The present study evaluated the tissue response to MTA that has been separately mixed with citric acid, calcium lactate gluconate (CLG), and Na2HPo4.
Materials and Method: In this experimental study Twenty one Wistar rats were divided into three groups of 7, 14 and 30 days follow up periods. Sterile polyethylene tubes were subsequently filled with MTA separately mixed with distilled water, 0.1% citric acid, 0.43% calcium lactate gluconate (CLG) and 15% Na2HPO4 and afterwards implanted subcutaneously. Empty tubes were implanted as negative control. At the end of their respective periods, the animals were sacrificed by anesthetic overdose and a biopsy was performed. The inflammatory responses were scored, classified and statistically analyzed using Kruskal-Wallis and Man-Whitney tests. Statistical significance was defined as p< 0.05.
Results: There was no significant difference between test groups in any time period after implantation but the mean values of inflammatory responses were significantly more than that of the negative control group (p> 0.05). The mean values of inflammatory responses were decreasing over time in all test groups. These values did not significantly differ in any group except the CLG and Na2HPO4 groups.
Conclusion: The inflammatory responses induced by MTA mixed with citric acid and MTA mixed with Na2HPo4 were comparable to that of the control MTA. MTA mixed with CLG provoked a moderate-to-severe inflammatory response at 7 days after implantation, so further study is required before clinical application of this cement. |
topic |
biocompatibility ; mineral trioxide aggregate ; accelerator |
url |
http://dentjods.sums.ac.ir/article_45894.html |
work_keys_str_mv |
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