Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements
Here, using cryo-EM and smFRET, Henderson et al. show how tryptophan 571 in the HIV-1 Env acts as a conformational switch during receptor-mediated viral entry and design HIV-1 Env proteins that cannot undergo conformational changes. This has important implications for HIV-1 vaccine design.
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2020-01-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-14196-w |
| id |
doaj-66b0bc924f5e41b8ab9751e365c7c967 |
|---|---|
| record_format |
Article |
| spelling |
doaj-66b0bc924f5e41b8ab9751e365c7c9672021-05-11T09:06:59ZengNature Publishing GroupNature Communications2041-17232020-01-0111111410.1038/s41467-019-14196-wDisruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangementsRory Henderson0Maolin Lu1Ye Zhou2Zekun Mu3Robert Parks4Qifeng Han5Allen L. Hsu6Elizabeth Carter7Scott C. Blanchard8R J Edwards9Kevin Wiehe10Kevin O. Saunders11Mario J. Borgnia12Alberto Bartesaghi13Walther Mothes14Barton F. Haynes15Priyamvada Acharya16S. Munir Alam17Department of Medicine, Duke University School of MedicineDepartment of Microbial Pathogenesis, Yale University School of MedicineDepartment of Computer Science, Duke UniversityDepartment of Immunology, Duke University School of MedicineDuke Human Vaccine Institute, Duke University School of MedicineDepartment of Medicine, Duke University School of MedicineGenome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human ServicesDepartment of Medicine, Duke University School of MedicineDepartment of Physiology and Biophysics, Weill Cornell MedicineDepartment of Medicine, Duke University School of MedicineDepartment of Medicine, Duke University School of MedicineDuke Human Vaccine Institute, Duke University School of MedicineGenome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human ServicesDepartment of Computer Science, Duke UniversityDepartment of Microbial Pathogenesis, Yale University School of MedicineDepartment of Medicine, Duke University School of MedicineDuke Human Vaccine Institute, Duke University School of MedicineDepartment of Medicine, Duke University School of MedicineHere, using cryo-EM and smFRET, Henderson et al. show how tryptophan 571 in the HIV-1 Env acts as a conformational switch during receptor-mediated viral entry and design HIV-1 Env proteins that cannot undergo conformational changes. This has important implications for HIV-1 vaccine design.https://doi.org/10.1038/s41467-019-14196-w |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Rory Henderson Maolin Lu Ye Zhou Zekun Mu Robert Parks Qifeng Han Allen L. Hsu Elizabeth Carter Scott C. Blanchard R J Edwards Kevin Wiehe Kevin O. Saunders Mario J. Borgnia Alberto Bartesaghi Walther Mothes Barton F. Haynes Priyamvada Acharya S. Munir Alam |
| spellingShingle |
Rory Henderson Maolin Lu Ye Zhou Zekun Mu Robert Parks Qifeng Han Allen L. Hsu Elizabeth Carter Scott C. Blanchard R J Edwards Kevin Wiehe Kevin O. Saunders Mario J. Borgnia Alberto Bartesaghi Walther Mothes Barton F. Haynes Priyamvada Acharya S. Munir Alam Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements Nature Communications |
| author_facet |
Rory Henderson Maolin Lu Ye Zhou Zekun Mu Robert Parks Qifeng Han Allen L. Hsu Elizabeth Carter Scott C. Blanchard R J Edwards Kevin Wiehe Kevin O. Saunders Mario J. Borgnia Alberto Bartesaghi Walther Mothes Barton F. Haynes Priyamvada Acharya S. Munir Alam |
| author_sort |
Rory Henderson |
| title |
Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements |
| title_short |
Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements |
| title_full |
Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements |
| title_fullStr |
Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements |
| title_full_unstemmed |
Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements |
| title_sort |
disruption of the hiv-1 envelope allosteric network blocks cd4-induced rearrangements |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2020-01-01 |
| description |
Here, using cryo-EM and smFRET, Henderson et al. show how tryptophan 571 in the HIV-1 Env acts as a conformational switch during receptor-mediated viral entry and design HIV-1 Env proteins that cannot undergo conformational changes. This has important implications for HIV-1 vaccine design. |
| url |
https://doi.org/10.1038/s41467-019-14196-w |
| work_keys_str_mv |
AT roryhenderson disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements AT maolinlu disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements AT yezhou disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements AT zekunmu disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements AT robertparks disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements AT qifenghan disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements AT allenlhsu disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements AT elizabethcarter disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements AT scottcblanchard disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements AT rjedwards disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements AT kevinwiehe disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements AT kevinosaunders disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements AT mariojborgnia disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements AT albertobartesaghi disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements AT walthermothes disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements AT bartonfhaynes disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements AT priyamvadaacharya disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements AT smuniralam disruptionofthehiv1envelopeallostericnetworkblockscd4inducedrearrangements |
| _version_ |
1721450209104363520 |