Recognition of Phytophthora infestans in potato (Solanum tuberosum L.): Scr74 gene as an example
Phytophthora infestans is the causal agent of late blight, the most devastating disease of potato worldwide. The P. infestans genome encodes potentially polymorphic genes that evolve continually to evade the recognition of plant R genes, though it has hundreds of predicted and conserved effector pro...
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Online Access: | https://doi.org/10.1515/opag-2016-0021 |
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doaj-66b4322b78cd43daadba7e56e4053bfc2021-09-05T20:51:14ZengDe GruyterOpen Agriculture2391-95312016-01-011115717210.1515/opag-2016-0021opag-2016-0021Recognition of Phytophthora infestans in potato (Solanum tuberosum L.): Scr74 gene as an exampleBitew Mulusew Kassa0Domazakis Emmanouil1Debre Markos University, Debre Markos, Po.Box: 269, Amhara, EthiopiaWageningen UR Plant Breeding, Wageningen University and Research, Centre. P.O. Box: 8 386, 6700 AJ, Wageningen, TheNetherlandsPhytophthora infestans is the causal agent of late blight, the most devastating disease of potato worldwide. The P. infestans genome encodes potentially polymorphic genes that evolve continually to evade the recognition of plant R genes, though it has hundreds of predicted and conserved effector proteins recognised by the plant. The gene Scr74 encodes a predicted 74-amino acid secreted cysteine-rich protein belonging to a highly polymorphic gene family within P. infestans. This study screened the recognition of Scr74 genes in wild potato genotypes from August 2013 to January 2014 in the Plant Breeding Laboratory of Wageningen University, the Netherlands. To identify the recognition of the Scr74 gene, we grew potato genotypes in the green house for PVX assays, detached leaf assays and molecular work. Twenty-seven good-quality sequences of the Scr74 gene variant with a length of 74 amino acids were found and more frequent amino acid variation was detected on the mature protein. Seventeen Scr74 constructs were identified as diversified and two effectors were strongly recognised by wild S. verrucosum genotypes via effectoromics from the PVX assay. A strong plant cell death hypersensitive response (HR) was recorded on wild S. verrucosum and S. tuberosum genotypes from the detached leaf assay. This recognition seems to be a useful indicator for the presence of a resistance gene (s) to the polymorphic effectors of P. infestans (as it has seen on Scr74 gene) in the wild potato genotypes.https://doi.org/10.1515/opag-2016-0021phytophthora infestans polymorphic gene phytotoxin-like protein scr74 gene apoplastic effectors effectoromics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bitew Mulusew Kassa Domazakis Emmanouil |
spellingShingle |
Bitew Mulusew Kassa Domazakis Emmanouil Recognition of Phytophthora infestans in potato (Solanum tuberosum L.): Scr74 gene as an example Open Agriculture phytophthora infestans polymorphic gene phytotoxin-like protein scr74 gene apoplastic effectors effectoromics |
author_facet |
Bitew Mulusew Kassa Domazakis Emmanouil |
author_sort |
Bitew Mulusew Kassa |
title |
Recognition of Phytophthora infestans in potato
(Solanum tuberosum L.): Scr74 gene as an
example |
title_short |
Recognition of Phytophthora infestans in potato
(Solanum tuberosum L.): Scr74 gene as an
example |
title_full |
Recognition of Phytophthora infestans in potato
(Solanum tuberosum L.): Scr74 gene as an
example |
title_fullStr |
Recognition of Phytophthora infestans in potato
(Solanum tuberosum L.): Scr74 gene as an
example |
title_full_unstemmed |
Recognition of Phytophthora infestans in potato
(Solanum tuberosum L.): Scr74 gene as an
example |
title_sort |
recognition of phytophthora infestans in potato
(solanum tuberosum l.): scr74 gene as an
example |
publisher |
De Gruyter |
series |
Open Agriculture |
issn |
2391-9531 |
publishDate |
2016-01-01 |
description |
Phytophthora infestans is the causal agent of late
blight, the most devastating disease of potato worldwide.
The P. infestans genome encodes potentially polymorphic
genes that evolve continually to evade the recognition of
plant R genes, though it has hundreds of predicted and
conserved effector proteins recognised by the plant. The
gene Scr74 encodes a predicted 74-amino acid secreted
cysteine-rich protein belonging to a highly polymorphic
gene family within P. infestans. This study screened the
recognition of Scr74 genes in wild potato genotypes
from August 2013 to January 2014 in the Plant Breeding
Laboratory of Wageningen University, the Netherlands. To
identify the recognition of the Scr74 gene, we grew potato
genotypes in the green house for PVX assays, detached leaf
assays and molecular work. Twenty-seven good-quality
sequences of the Scr74 gene variant with a length of 74
amino acids were found and more frequent amino acid
variation was detected on the mature protein. Seventeen
Scr74 constructs were identified as diversified and two
effectors were strongly recognised by wild S. verrucosum
genotypes via effectoromics from the PVX assay. A strong
plant cell death hypersensitive response (HR) was recorded
on wild S. verrucosum and S. tuberosum genotypes from
the detached leaf assay. This recognition seems to be a
useful indicator for the presence of a resistance gene (s) to
the polymorphic effectors of P. infestans (as it has seen on
Scr74 gene) in the wild potato genotypes. |
topic |
phytophthora infestans polymorphic gene phytotoxin-like protein scr74 gene apoplastic effectors effectoromics |
url |
https://doi.org/10.1515/opag-2016-0021 |
work_keys_str_mv |
AT bitewmulusewkassa recognitionofphytophthorainfestansinpotatosolanumtuberosumlscr74geneasanexample AT domazakisemmanouil recognitionofphytophthorainfestansinpotatosolanumtuberosumlscr74geneasanexample |
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1717783983455993856 |