Size and Density of Artemisia annua Stomata Soaked in Water Extract of Gloriosa superba Seeds

• Main Authors: Sri Indah Rahmawati, Ahmad Yunus, Ari Susilowati
• Format: Article
• Language: English
• Published: Universitas Negeri Semarang 2017-12-01
• Series: Biosaintifika: Journal of Biology & Biology Education
• Subjects: Size; Density; Stomata; Artemisia annua; Gloriosa superba
• Online Access: https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/11234
• ISSN: 2085-191X; 2338-7610

<p class="IsiAbstrakIndo"><em><span lang="EN-GB">Artemisia annua</span></em><span lang="EN-GB"> is a herbaceous plant that produces artemisinin as a malaria drug, haemorrhoids therapy, aromatherapy, antiviral, anticancer and antibacterial. </span><em><span lang="EN-GB">Gloriosa superba</span></em><span lang="EN-GB"> is a plant that contains high colchicine compounds, especially on the seeds. </span><em><span lang="EN-GB">Gloriosa superba</span></em><span lang="EN-GB"> extracts of tubers, stems, seeds, and leaves were used as biomutagen for many plants. Colchicine contains of these plants as antimitotic have been studied and proven by the mitotic index plants. Water extracts of </span><em><span lang="EN-GB">Gloriosa superba</span></em><span lang="EN-GB"> seeds was used as a mutagen for </span><em><span lang="EN-GB">Artemisia annua</span></em><span lang="EN-GB">. The aim of this study was to determine the size and density of </span><em><span lang="EN-GB">Artemisia annua</span></em><span lang="EN-GB"> stomata soaked in water extract of </span><em><span lang="EN-GB">Gloriosa superba</span></em><span lang="EN-GB"> seeds as a mutagen. Extraction of </span><em><span lang="EN-GB">Gloriosa superba</span></em><span lang="EN-GB"> seeds obtained naturally on Krakal Beach, Gunung Kidul by using a maceration method with water solvent (1:1). </span><em><span lang="EN-GB">Artemisia annua</span></em><span lang="EN-GB"> sprouts were obtained from B2P2TOOT Tawangmangu. Variables treatment on sprouts using water extract concentration of </span><em><span lang="EN-GB">Gloriosa superba</span></em><span lang="EN-GB"> seeds and soaking time of </span><em><span lang="EN-GB">Artemisia annua</span></em><span lang="EN-GB"> sprouts. Measurements of stomatal length, width and density were conducted in epidermis of </span><em><span lang="EN-GB">Artemisia annua</span></em><span lang="EN-GB"> leaf. Observation and measurements of the stomata were conducted by using a light microscope. The results showed that the length and width of stomata were 0.025 mm and 0.017 mm respectively. The stomatal density of the control leaf (174.69 amount/mm2) was lower than the other treated plants. Stomatal size and density has increased with the increasing concentration extracts on treated plants. Water extracts of </span><em><span lang="EN-GB">Gloriosa superba</span></em><span lang="EN-GB"> seeds proved the effects on stomatal size and density of treated plants.  </span></p>