Increased Expression of Thymic Stromal Lymphopoietin in Chronic Constriction Injury of Rat Nerve
Thymic stromal lymphopoietin (TSLP) is a well-known cytokine for T helper 2 inflammatory responses. A nerve injury activates the neuroinflammation cascade and neuron–glia interaction in dorsal root ganglions (DRG)s, leading to neuropathic pain. Therefore, this study was to investigate the role of TS...
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doaj-66c46fe7a13f4d568746f3e5fc1e4ada2021-07-15T15:38:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-01227105710510.3390/ijms22137105Increased Expression of Thymic Stromal Lymphopoietin in Chronic Constriction Injury of Rat NerveChieh-Hsin Wu0Chun-Ching Lu1Chao-Lan Huang2Ming-Kung Wu3Ying-Yi Lu4Division of Neurosurgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung 807, TaiwanDepartment of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei 112, TaiwanDepartment of Anesthesiology, Taipei Veterans General Hospital, Taipei 112, TaiwanDepartment of Psychiatry, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanDepartment of Dermatology, Kaohsiung Veterans General Hospital, Kaohsiung 813, TaiwanThymic stromal lymphopoietin (TSLP) is a well-known cytokine for T helper 2 inflammatory responses. A nerve injury activates the neuroinflammation cascade and neuron–glia interaction in dorsal root ganglions (DRG)s, leading to neuropathic pain. Therefore, this study was to investigate the role of TSLP after nerve injury. Male Sprague-Dawley rats were divided as an experimental group with chronic constriction injury (CCI) to the sciatic nerve and a control group. The mechanical pain threshold response was determined by calibration forceps. After assessment of mechanical allodynia, the ipsilateral spinal cord, DRG, sciatic nerve and skin were harvested. Immunofluorescence staining was performed to identify cell types with various markers. Western blot analyses were performed to evaluate protein expressions. Mechanical allodynia developed after CCI and persisted for the next 14 days. Astrocyte reactions occurred and continued until day 14, too. After CCI, DRG and the sciatic nerve also had significantly increased expressions of TSLP/TSLP-R/STAT5. The TSLPR was localized to sensory neuronal endings innervating the skin. This study is the first to demonstrate that the TSLP complex and the STAT5 pathway in nerve are potential therapeutic targets because of their roles in pain regulation after nerve injury.https://www.mdpi.com/1422-0067/22/13/7105thymic stromal lymphopoietin (TSLP)dorsal root ganglions (DRG)neuropathic painchronic constriction injury (CCI) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chieh-Hsin Wu Chun-Ching Lu Chao-Lan Huang Ming-Kung Wu Ying-Yi Lu |
spellingShingle |
Chieh-Hsin Wu Chun-Ching Lu Chao-Lan Huang Ming-Kung Wu Ying-Yi Lu Increased Expression of Thymic Stromal Lymphopoietin in Chronic Constriction Injury of Rat Nerve International Journal of Molecular Sciences thymic stromal lymphopoietin (TSLP) dorsal root ganglions (DRG) neuropathic pain chronic constriction injury (CCI) |
author_facet |
Chieh-Hsin Wu Chun-Ching Lu Chao-Lan Huang Ming-Kung Wu Ying-Yi Lu |
author_sort |
Chieh-Hsin Wu |
title |
Increased Expression of Thymic Stromal Lymphopoietin in Chronic Constriction Injury of Rat Nerve |
title_short |
Increased Expression of Thymic Stromal Lymphopoietin in Chronic Constriction Injury of Rat Nerve |
title_full |
Increased Expression of Thymic Stromal Lymphopoietin in Chronic Constriction Injury of Rat Nerve |
title_fullStr |
Increased Expression of Thymic Stromal Lymphopoietin in Chronic Constriction Injury of Rat Nerve |
title_full_unstemmed |
Increased Expression of Thymic Stromal Lymphopoietin in Chronic Constriction Injury of Rat Nerve |
title_sort |
increased expression of thymic stromal lymphopoietin in chronic constriction injury of rat nerve |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-07-01 |
description |
Thymic stromal lymphopoietin (TSLP) is a well-known cytokine for T helper 2 inflammatory responses. A nerve injury activates the neuroinflammation cascade and neuron–glia interaction in dorsal root ganglions (DRG)s, leading to neuropathic pain. Therefore, this study was to investigate the role of TSLP after nerve injury. Male Sprague-Dawley rats were divided as an experimental group with chronic constriction injury (CCI) to the sciatic nerve and a control group. The mechanical pain threshold response was determined by calibration forceps. After assessment of mechanical allodynia, the ipsilateral spinal cord, DRG, sciatic nerve and skin were harvested. Immunofluorescence staining was performed to identify cell types with various markers. Western blot analyses were performed to evaluate protein expressions. Mechanical allodynia developed after CCI and persisted for the next 14 days. Astrocyte reactions occurred and continued until day 14, too. After CCI, DRG and the sciatic nerve also had significantly increased expressions of TSLP/TSLP-R/STAT5. The TSLPR was localized to sensory neuronal endings innervating the skin. This study is the first to demonstrate that the TSLP complex and the STAT5 pathway in nerve are potential therapeutic targets because of their roles in pain regulation after nerve injury. |
topic |
thymic stromal lymphopoietin (TSLP) dorsal root ganglions (DRG) neuropathic pain chronic constriction injury (CCI) |
url |
https://www.mdpi.com/1422-0067/22/13/7105 |
work_keys_str_mv |
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