Comparing the efficacy of leishman–giemsa cocktail stain, giemsa stain, and papanicolaou stain in potentially malignant oral lesions: A study on 540 cytological samples
Background: This study was planned to compare and evaluate the staining efficacy of Leishman–Giemsa cocktail (LG), Papanicolaou, and Giemsa stain (G) in potentially malignant disorders and malignant lesions. Aims: To evaluate the quality of nuclear and cytoplasmic staining of LG with G, and rapid Pa...
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Wolters Kluwer Medknow Publications
2018-01-01
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doaj-66ce398f66ec4859803c3eb751e1e1f42020-11-24T23:24:29ZengWolters Kluwer Medknow PublicationsJournal of Cytology0970-93712018-01-0135210510910.4103/JOC.JOC_249_16Comparing the efficacy of leishman–giemsa cocktail stain, giemsa stain, and papanicolaou stain in potentially malignant oral lesions: A study on 540 cytological samplesSupreet K SidhuKarthikeyan RamalingamSandeep GoyalMonika PooniaGaurav S RajawatNitish SharmaBackground: This study was planned to compare and evaluate the staining efficacy of Leishman–Giemsa cocktail (LG), Papanicolaou, and Giemsa stain (G) in potentially malignant disorders and malignant lesions. Aims: To evaluate the quality of nuclear and cytoplasmic staining of LG with G, and rapid Papanicolaou stain (Pap) and to compare the total staining efficiency of LG against G and P. Materials and Methods: One hundred and eighty participants were studied under three groups – 60 as healthy controls, 60 with potentially malignant disorders, and 60 with malignant lesions; smears were taken thrice from the buccal mucosa. One smear was fixed with Bio-Fix spray and other two smears were allowed to air dry for 2–3 minutes. Then, the ethyl alcohol-fixed smear was stained with Pap and the two other air-dried smears were stained with G and LG stains. Analysis was done using Friedman test and Wilcoxon Signed Rank Test with SPSS Version 15.0. Results: In the normal group, staining of LG was highly significant (P < 0.001). Among potentially malignant lesions, LG was observed to be highly significant (P < 0.001) when compared with G and was not significant when compared with Pap (P = 0.186). In the malignant group, LG was highly significant (P < 0.001). LG was superior with the highest average staining score of (2.018) than Pap and G. Conclusion: LG cocktail is a better stain with excellent cytoplasmic and nuclear staining intensity compared to Pap and G stains.http://www.jcytol.org/article.asp?issn=0970-9371;year=2018;volume=35;issue=2;spage=105;epage=109;aulast=SidhuExfoliative cytologyGiemsa stainLeishman–Giemsa cocktail stainrapid Papanicolaou methodstaining efficiency |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Supreet K Sidhu Karthikeyan Ramalingam Sandeep Goyal Monika Poonia Gaurav S Rajawat Nitish Sharma |
spellingShingle |
Supreet K Sidhu Karthikeyan Ramalingam Sandeep Goyal Monika Poonia Gaurav S Rajawat Nitish Sharma Comparing the efficacy of leishman–giemsa cocktail stain, giemsa stain, and papanicolaou stain in potentially malignant oral lesions: A study on 540 cytological samples Journal of Cytology Exfoliative cytology Giemsa stain Leishman–Giemsa cocktail stain rapid Papanicolaou method staining efficiency |
author_facet |
Supreet K Sidhu Karthikeyan Ramalingam Sandeep Goyal Monika Poonia Gaurav S Rajawat Nitish Sharma |
author_sort |
Supreet K Sidhu |
title |
Comparing the efficacy of leishman–giemsa cocktail stain, giemsa stain, and papanicolaou stain in potentially malignant oral lesions: A study on 540 cytological samples |
title_short |
Comparing the efficacy of leishman–giemsa cocktail stain, giemsa stain, and papanicolaou stain in potentially malignant oral lesions: A study on 540 cytological samples |
title_full |
Comparing the efficacy of leishman–giemsa cocktail stain, giemsa stain, and papanicolaou stain in potentially malignant oral lesions: A study on 540 cytological samples |
title_fullStr |
Comparing the efficacy of leishman–giemsa cocktail stain, giemsa stain, and papanicolaou stain in potentially malignant oral lesions: A study on 540 cytological samples |
title_full_unstemmed |
Comparing the efficacy of leishman–giemsa cocktail stain, giemsa stain, and papanicolaou stain in potentially malignant oral lesions: A study on 540 cytological samples |
title_sort |
comparing the efficacy of leishman–giemsa cocktail stain, giemsa stain, and papanicolaou stain in potentially malignant oral lesions: a study on 540 cytological samples |
publisher |
Wolters Kluwer Medknow Publications |
series |
Journal of Cytology |
issn |
0970-9371 |
publishDate |
2018-01-01 |
description |
Background: This study was planned to compare and evaluate the staining efficacy of Leishman–Giemsa cocktail (LG), Papanicolaou, and Giemsa stain (G) in potentially malignant disorders and malignant lesions. Aims: To evaluate the quality of nuclear and cytoplasmic staining of LG with G, and rapid Papanicolaou stain (Pap) and to compare the total staining efficiency of LG against G and P. Materials and Methods: One hundred and eighty participants were studied under three groups – 60 as healthy controls, 60 with potentially malignant disorders, and 60 with malignant lesions; smears were taken thrice from the buccal mucosa. One smear was fixed with Bio-Fix spray and other two smears were allowed to air dry for 2–3 minutes. Then, the ethyl alcohol-fixed smear was stained with Pap and the two other air-dried smears were stained with G and LG stains. Analysis was done using Friedman test and Wilcoxon Signed Rank Test with SPSS Version 15.0. Results: In the normal group, staining of LG was highly significant (P < 0.001). Among potentially malignant lesions, LG was observed to be highly significant (P < 0.001) when compared with G and was not significant when compared with Pap (P = 0.186). In the malignant group, LG was highly significant (P < 0.001). LG was superior with the highest average staining score of (2.018) than Pap and G. Conclusion: LG cocktail is a better stain with excellent cytoplasmic and nuclear staining intensity compared to Pap and G stains. |
topic |
Exfoliative cytology Giemsa stain Leishman–Giemsa cocktail stain rapid Papanicolaou method staining efficiency |
url |
http://www.jcytol.org/article.asp?issn=0970-9371;year=2018;volume=35;issue=2;spage=105;epage=109;aulast=Sidhu |
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