miR-196a-2 Promotes Malignant Progression of Thyroid Carcinoma by Targeting NRXN1
Thyroid cancer (TC) is the most common endocrine malignant disease with a rising morbidity year by year. Accumulating studies have shown that microRNAs (miRNAs) play a regulatory role in the progression of various tumors, but the molecular regulatory mechanism of miR-196a-2 in TC is still unknown. q...
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doaj-66d3b96026034ba4b3ee44b1dd152eab2021-06-14T00:17:17ZengHindawi LimitedComputational and Mathematical Methods in Medicine1748-67182021-01-01202110.1155/2021/4856820miR-196a-2 Promotes Malignant Progression of Thyroid Carcinoma by Targeting NRXN1Yaohua Fan0MingJian Fei1Yan Li2Zhenzhen Gao3Yuzhang Zhu4Guiping Dai5Dongjuan Wu6Department of OncologyDepartment of PathologyDepartment of OncologyDepartment of OncologyDepartment of OncologyDepartment of OncologyDepartment of OncologyThyroid cancer (TC) is the most common endocrine malignant disease with a rising morbidity year by year. Accumulating studies have shown that microRNAs (miRNAs) play a regulatory role in the progression of various tumors, but the molecular regulatory mechanism of miR-196a-2 in TC is still unknown. qRT-PCR was employed to measure the expression of miR-196a-2 and NRXN1 mRNA in TC cells, while western blot was used to detect the protein expression of NRXN1. CCK-8, colony formation and flow cytometry assays were used to measure cell proliferation and apoptosis of TC cells. Dual-luciferase reporter gene assay was used to predict and verify the targeted binding relationship between miR-196a-2 and NRXN1. Our study results manifested that miR-196a-2 was dramatically overexpressed in cells of TC, while NRXN1 was lowly expressed. miR-196a-2 could promote cell proliferation and inhibit cell apoptosis of TC. Additionally, miR-196a-2 could also target and inhibit the expression of NRXN1. Silencing NRXN1 could reverse the inhibitory effect of miR-196a-2 downregulation on cell proliferation of TC, as well as the promoting effect on cell apoptosis. In a conclusion, we found that miR-196a-2 could promote cell proliferation and inhibit cell apoptosis of TC by targeting NRXN1. Therefore, miR-196a-2/NRXN1 is potential to be a molecular therapeutic target for TC.http://dx.doi.org/10.1155/2021/4856820 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yaohua Fan MingJian Fei Yan Li Zhenzhen Gao Yuzhang Zhu Guiping Dai Dongjuan Wu |
spellingShingle |
Yaohua Fan MingJian Fei Yan Li Zhenzhen Gao Yuzhang Zhu Guiping Dai Dongjuan Wu miR-196a-2 Promotes Malignant Progression of Thyroid Carcinoma by Targeting NRXN1 Computational and Mathematical Methods in Medicine |
author_facet |
Yaohua Fan MingJian Fei Yan Li Zhenzhen Gao Yuzhang Zhu Guiping Dai Dongjuan Wu |
author_sort |
Yaohua Fan |
title |
miR-196a-2 Promotes Malignant Progression of Thyroid Carcinoma by Targeting NRXN1 |
title_short |
miR-196a-2 Promotes Malignant Progression of Thyroid Carcinoma by Targeting NRXN1 |
title_full |
miR-196a-2 Promotes Malignant Progression of Thyroid Carcinoma by Targeting NRXN1 |
title_fullStr |
miR-196a-2 Promotes Malignant Progression of Thyroid Carcinoma by Targeting NRXN1 |
title_full_unstemmed |
miR-196a-2 Promotes Malignant Progression of Thyroid Carcinoma by Targeting NRXN1 |
title_sort |
mir-196a-2 promotes malignant progression of thyroid carcinoma by targeting nrxn1 |
publisher |
Hindawi Limited |
series |
Computational and Mathematical Methods in Medicine |
issn |
1748-6718 |
publishDate |
2021-01-01 |
description |
Thyroid cancer (TC) is the most common endocrine malignant disease with a rising morbidity year by year. Accumulating studies have shown that microRNAs (miRNAs) play a regulatory role in the progression of various tumors, but the molecular regulatory mechanism of miR-196a-2 in TC is still unknown. qRT-PCR was employed to measure the expression of miR-196a-2 and NRXN1 mRNA in TC cells, while western blot was used to detect the protein expression of NRXN1. CCK-8, colony formation and flow cytometry assays were used to measure cell proliferation and apoptosis of TC cells. Dual-luciferase reporter gene assay was used to predict and verify the targeted binding relationship between miR-196a-2 and NRXN1. Our study results manifested that miR-196a-2 was dramatically overexpressed in cells of TC, while NRXN1 was lowly expressed. miR-196a-2 could promote cell proliferation and inhibit cell apoptosis of TC. Additionally, miR-196a-2 could also target and inhibit the expression of NRXN1. Silencing NRXN1 could reverse the inhibitory effect of miR-196a-2 downregulation on cell proliferation of TC, as well as the promoting effect on cell apoptosis. In a conclusion, we found that miR-196a-2 could promote cell proliferation and inhibit cell apoptosis of TC by targeting NRXN1. Therefore, miR-196a-2/NRXN1 is potential to be a molecular therapeutic target for TC. |
url |
http://dx.doi.org/10.1155/2021/4856820 |
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