Molecular hydrogen suppresses glioblastoma growth via inducing the glioma stem-like cell differentiation
Abstract Background Glioblastoma (GBM) is the most common type of primary malignant brain tumor. Molecular hydrogen has been considered a preventive and therapeutic medical gas in many diseases including cancer. In our study, we sought to assess the potential role of molecular hydrogen on GBM. Metho...
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doaj-66e3637765be49e9a0a514f1603870b32020-11-25T03:48:44ZengBMCStem Cell Research & Therapy1757-65122019-05-0110111010.1186/s13287-019-1241-xMolecular hydrogen suppresses glioblastoma growth via inducing the glioma stem-like cell differentiationMeng-yu Liu0Fei Xie1Yan Zhang2Ting-ting Wang3Sheng-nan Ma4Peng-xiang Zhao5Xin Zhang6Tyler W. Lebaron7Xin-long Yan8Xue-mei Ma9College of Life Science and Bio-engineering, Beijing University of TechnologyCollege of Life Science and Bio-engineering, Beijing University of TechnologyAffiliated Bayi Brain Hospital, The Seventh Medical Center of PLA General HospitalCollege of Life Science and Bio-engineering, Beijing University of TechnologyCollege of Life Science and Bio-engineering, Beijing University of TechnologyCollege of Life Science and Bio-engineering, Beijing University of TechnologyCollege of Life Science and Bio-engineering, Beijing University of TechnologyCorrection is Molecular Hydrogen InstituteCollege of Life Science and Bio-engineering, Beijing University of TechnologyCollege of Life Science and Bio-engineering, Beijing University of TechnologyAbstract Background Glioblastoma (GBM) is the most common type of primary malignant brain tumor. Molecular hydrogen has been considered a preventive and therapeutic medical gas in many diseases including cancer. In our study, we sought to assess the potential role of molecular hydrogen on GBM. Methods The in vivo studies were performed using a rat orthotopic glioma model and a mouse subcutaneous xenograft model. Animals inhaled hydrogen gas (67%) 1 h two times per day. MR imaging studies were performed to determine the tumor volume. Immunohistochemistry (IHC), immunofluorescence staining, and flow cytometry analysis were conducted to determine the expression of surface markers. Sphere formation assay was performed to assess the cancer stem cell self-renewal capacity. Assays for cell migration, invasion, and colony formation were conducted. Results The in vivo study showed that hydrogen inhalation could effectively suppress GBM tumor growth and prolong the survival of mice with GBM. IHC and immunofluorescence staining demonstrated that hydrogen treatment markedly downregulated the expression of markers involved in stemness (CD133, Nestin), proliferation (ki67), and angiogenesis (CD34) and also upregulated GFAP expression, a marker of differentiation. Similar results were obtained in the in vitro studies. The sphere-forming ability of glioma cells was also suppressed by hydrogen treatment. Moreover, hydrogen treatment also suppressed the migration, invasion, and colony-forming ability of glioma cells. Conclusions Together, these results indicated that molecular hydrogen may serve as a potential anti-tumor agent in the treatment of GBM.http://link.springer.com/article/10.1186/s13287-019-1241-xMolecular hydrogenGlioblastomaGlioma stem-like cellCancer cell stemness |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Meng-yu Liu Fei Xie Yan Zhang Ting-ting Wang Sheng-nan Ma Peng-xiang Zhao Xin Zhang Tyler W. Lebaron Xin-long Yan Xue-mei Ma |
spellingShingle |
Meng-yu Liu Fei Xie Yan Zhang Ting-ting Wang Sheng-nan Ma Peng-xiang Zhao Xin Zhang Tyler W. Lebaron Xin-long Yan Xue-mei Ma Molecular hydrogen suppresses glioblastoma growth via inducing the glioma stem-like cell differentiation Stem Cell Research & Therapy Molecular hydrogen Glioblastoma Glioma stem-like cell Cancer cell stemness |
author_facet |
Meng-yu Liu Fei Xie Yan Zhang Ting-ting Wang Sheng-nan Ma Peng-xiang Zhao Xin Zhang Tyler W. Lebaron Xin-long Yan Xue-mei Ma |
author_sort |
Meng-yu Liu |
title |
Molecular hydrogen suppresses glioblastoma growth via inducing the glioma stem-like cell differentiation |
title_short |
Molecular hydrogen suppresses glioblastoma growth via inducing the glioma stem-like cell differentiation |
title_full |
Molecular hydrogen suppresses glioblastoma growth via inducing the glioma stem-like cell differentiation |
title_fullStr |
Molecular hydrogen suppresses glioblastoma growth via inducing the glioma stem-like cell differentiation |
title_full_unstemmed |
Molecular hydrogen suppresses glioblastoma growth via inducing the glioma stem-like cell differentiation |
title_sort |
molecular hydrogen suppresses glioblastoma growth via inducing the glioma stem-like cell differentiation |
publisher |
BMC |
series |
Stem Cell Research & Therapy |
issn |
1757-6512 |
publishDate |
2019-05-01 |
description |
Abstract Background Glioblastoma (GBM) is the most common type of primary malignant brain tumor. Molecular hydrogen has been considered a preventive and therapeutic medical gas in many diseases including cancer. In our study, we sought to assess the potential role of molecular hydrogen on GBM. Methods The in vivo studies were performed using a rat orthotopic glioma model and a mouse subcutaneous xenograft model. Animals inhaled hydrogen gas (67%) 1 h two times per day. MR imaging studies were performed to determine the tumor volume. Immunohistochemistry (IHC), immunofluorescence staining, and flow cytometry analysis were conducted to determine the expression of surface markers. Sphere formation assay was performed to assess the cancer stem cell self-renewal capacity. Assays for cell migration, invasion, and colony formation were conducted. Results The in vivo study showed that hydrogen inhalation could effectively suppress GBM tumor growth and prolong the survival of mice with GBM. IHC and immunofluorescence staining demonstrated that hydrogen treatment markedly downregulated the expression of markers involved in stemness (CD133, Nestin), proliferation (ki67), and angiogenesis (CD34) and also upregulated GFAP expression, a marker of differentiation. Similar results were obtained in the in vitro studies. The sphere-forming ability of glioma cells was also suppressed by hydrogen treatment. Moreover, hydrogen treatment also suppressed the migration, invasion, and colony-forming ability of glioma cells. Conclusions Together, these results indicated that molecular hydrogen may serve as a potential anti-tumor agent in the treatment of GBM. |
topic |
Molecular hydrogen Glioblastoma Glioma stem-like cell Cancer cell stemness |
url |
http://link.springer.com/article/10.1186/s13287-019-1241-x |
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