Comparison of Experimental Diabetic Periodontitis Induced by Porphyromonas gingivalis in Mice
Periodontitis is one of the severe complications in diabetic patients and gingival epithelium plays an initial role on the onset and progression of this disease. However the potential mechanism is yet sufficiently understood. Meanwhile, the research on the correlational experimental animal models wa...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2016-01-01
|
Series: | Journal of Diabetes Research |
Online Access: | http://dx.doi.org/10.1155/2016/4840203 |
id |
doaj-67020eea06d741b0a3f3cd91766a332a |
---|---|
record_format |
Article |
spelling |
doaj-67020eea06d741b0a3f3cd91766a332a2020-11-25T00:26:06ZengHindawi LimitedJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/48402034840203Comparison of Experimental Diabetic Periodontitis Induced by Porphyromonas gingivalis in MiceQi Wang0Peng Zhang1Ray Aprecio2Dongjiao Zhang3Hao Li4Ning Ji5Omaima Mohamed6Wu Zhang7Yiming Li8Yi Ding9State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Section S. Renmin Road, Chengdu, ChinaState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Section S. Renmin Road, Chengdu, ChinaCenter for Dental Research, School of Dentistry, Loma Linda University, 11175 Campus Street, Loma Linda, CA, USAShandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, 44-1 Wenhua W. Road, Jinan, ChinaDepartment of Prosthodontics, The Affiliated Hospital of Stomatology, Guangxi Medical University, 10 Shuangyong Road, Nanning 530021, ChinaState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Section S. Renmin Road, Chengdu, ChinaCenter for Dental Research, School of Dentistry, Loma Linda University, 11175 Campus Street, Loma Linda, CA, USACenter for Dental Research, School of Dentistry, Loma Linda University, 11175 Campus Street, Loma Linda, CA, USACenter for Dental Research, School of Dentistry, Loma Linda University, 11175 Campus Street, Loma Linda, CA, USAState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Section S. Renmin Road, Chengdu, ChinaPeriodontitis is one of the severe complications in diabetic patients and gingival epithelium plays an initial role on the onset and progression of this disease. However the potential mechanism is yet sufficiently understood. Meanwhile, the research on the correlational experimental animal models was also insufficient. Here, we established periodontitis with type 2 diabetes in db/db and Tallyho/JngJ (TH) mice and periodontitis with type 1 diabetes in streptozotocin induced diabetes C57BL/6J (STZ-C57) mice by oral infection of periodontal pathogen Porphyromonas gingivalis W50. We demonstrated that periodontal infected mice with high blood glucose levels showed dramatically more alveolar bone loss than their counterparts, in which infected db/db mice exhibited the most bone defects. No contrary impact could be observed between this periodontal infection and onset and severity of diabetes. The expressions of PTPN2 were inhibited whereas the expression of JAK1, STAT1, and STAT3 increased dramatically in gingival epithelia and the serum TNF-α also significantly increased in the mice with diabetic periodontitis. Our results indicated that the variations of inflammation-related protein expressions in gingival epithelia might lead to the phenotype differences in the mice with diabetic periodontitis.http://dx.doi.org/10.1155/2016/4840203 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qi Wang Peng Zhang Ray Aprecio Dongjiao Zhang Hao Li Ning Ji Omaima Mohamed Wu Zhang Yiming Li Yi Ding |
spellingShingle |
Qi Wang Peng Zhang Ray Aprecio Dongjiao Zhang Hao Li Ning Ji Omaima Mohamed Wu Zhang Yiming Li Yi Ding Comparison of Experimental Diabetic Periodontitis Induced by Porphyromonas gingivalis in Mice Journal of Diabetes Research |
author_facet |
Qi Wang Peng Zhang Ray Aprecio Dongjiao Zhang Hao Li Ning Ji Omaima Mohamed Wu Zhang Yiming Li Yi Ding |
author_sort |
Qi Wang |
title |
Comparison of Experimental Diabetic Periodontitis Induced by Porphyromonas gingivalis in Mice |
title_short |
Comparison of Experimental Diabetic Periodontitis Induced by Porphyromonas gingivalis in Mice |
title_full |
Comparison of Experimental Diabetic Periodontitis Induced by Porphyromonas gingivalis in Mice |
title_fullStr |
Comparison of Experimental Diabetic Periodontitis Induced by Porphyromonas gingivalis in Mice |
title_full_unstemmed |
Comparison of Experimental Diabetic Periodontitis Induced by Porphyromonas gingivalis in Mice |
title_sort |
comparison of experimental diabetic periodontitis induced by porphyromonas gingivalis in mice |
publisher |
Hindawi Limited |
series |
Journal of Diabetes Research |
issn |
2314-6745 2314-6753 |
publishDate |
2016-01-01 |
description |
Periodontitis is one of the severe complications in diabetic patients and gingival epithelium plays an initial role on the onset and progression of this disease. However the potential mechanism is yet sufficiently understood. Meanwhile, the research on the correlational experimental animal models was also insufficient. Here, we established periodontitis with type 2 diabetes in db/db and Tallyho/JngJ (TH) mice and periodontitis with type 1 diabetes in streptozotocin induced diabetes C57BL/6J (STZ-C57) mice by oral infection of periodontal pathogen Porphyromonas gingivalis W50. We demonstrated that periodontal infected mice with high blood glucose levels showed dramatically more alveolar bone loss than their counterparts, in which infected db/db mice exhibited the most bone defects. No contrary impact could be observed between this periodontal infection and onset and severity of diabetes. The expressions of PTPN2 were inhibited whereas the expression of JAK1, STAT1, and STAT3 increased dramatically in gingival epithelia and the serum TNF-α also significantly increased in the mice with diabetic periodontitis. Our results indicated that the variations of inflammation-related protein expressions in gingival epithelia might lead to the phenotype differences in the mice with diabetic periodontitis. |
url |
http://dx.doi.org/10.1155/2016/4840203 |
work_keys_str_mv |
AT qiwang comparisonofexperimentaldiabeticperiodontitisinducedbyporphyromonasgingivalisinmice AT pengzhang comparisonofexperimentaldiabeticperiodontitisinducedbyporphyromonasgingivalisinmice AT rayaprecio comparisonofexperimentaldiabeticperiodontitisinducedbyporphyromonasgingivalisinmice AT dongjiaozhang comparisonofexperimentaldiabeticperiodontitisinducedbyporphyromonasgingivalisinmice AT haoli comparisonofexperimentaldiabeticperiodontitisinducedbyporphyromonasgingivalisinmice AT ningji comparisonofexperimentaldiabeticperiodontitisinducedbyporphyromonasgingivalisinmice AT omaimamohamed comparisonofexperimentaldiabeticperiodontitisinducedbyporphyromonasgingivalisinmice AT wuzhang comparisonofexperimentaldiabeticperiodontitisinducedbyporphyromonasgingivalisinmice AT yimingli comparisonofexperimentaldiabeticperiodontitisinducedbyporphyromonasgingivalisinmice AT yiding comparisonofexperimentaldiabeticperiodontitisinducedbyporphyromonasgingivalisinmice |
_version_ |
1725345944684199936 |