Study of Callus Induction and Shoot Regeneration of Safflower (Carthamus tinctorius L.) Using Hypocotyl and Cotyledon Explants Culture
After cereals, oilseed crops are included as the second source of supplying calorie for human populations. In recent years, safflower was considered due to quality and high content of fatty acids. For optimization of tissue culture of safflower, an experiment was carried out as a factorial arrangeme...
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Shahid Bahonar University of Kerman
2010-05-01
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Online Access: | https://jab.uk.ac.ir/article_364_a962cdc2e97751460a4658a999119645.pdf |
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doaj-67106b82ce024cf496514af1ae9dd6e62020-11-25T02:58:37ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002010-05-01219911110.22103/jab.2011.364364Study of Callus Induction and Shoot Regeneration of Safflower (Carthamus tinctorius L.) Using Hypocotyl and Cotyledon Explants Culturejavad Motamedi0Alireza zebarjadi1Danyal kahrizi2Alihatef salmanian3jaleh soheylikhah4دانشجوی کارشناسی ارشد اصلاح نباتات گروه زراعت و اصلاح نباتات دانشکده کشاورزی دانشگاه رازی کرمانشاه دانشگاه آزاداسلامی واحد کرمانشاه باشگاه پژوهشگران جوان کرمانشاه ایراناستادیار گروه زراعت و اصلاح نباتات دانشکده کاورزی دانشگاه رازی کرمانشاه-استادیار گروه پژوهشی بیوتکنولوژی مقاومت به خشکی دانشگاه رازی کرمانشاهاستادیار گروه زراعت و اصلاح نباتات دانشکده کشاورزی دانشگاه رازی کرمانشاه- استادیار گروه پژوهشی بیوتکنولوژی مقاومت به خشکی دانشگاه رازی کرمانشاهدانشیار پژوهشگاه ملی تحقیقات مهندسی ژنتیک و زیست فن آوری تهرانکارشناس ارشد زیست شناسی دانشکده علوم دانشگاه رازی کرمانشاهAfter cereals, oilseed crops are included as the second source of supplying calorie for human populations. In recent years, safflower was considered due to quality and high content of fatty acids. For optimization of tissue culture of safflower, an experiment was carried out as a factorial arrangement in a completely randomized design with three replications in 2008-2009. Callus induction and shoot regeneration percentage of cotyledons and hypocotyl explants were measured on basal MS medium for two cultivars (Dincer and Sina) of safflower in different concentrations of NAA and BAP hormones. Analysis of interaction effects indicated that the cultivars were independent in response of different level of NAA. Mean comparison showed that the highest percentage of callus induction occurred on MS medium containing 0.5 mg/l NAA and 0.5 mg/l BAP (94.33%) and 1 mg/l NAA and 1 mg/l BAP (97%). Furthermore, hypocotyl explant of Dincer has a good response to callus induction. The highest percentage of shoot regeneration also was achieved on MS medium supplemented with 0.1 mg/l NAA and 2 mg/l BAP from cotyledon explant of Dincer.https://jab.uk.ac.ir/article_364_a962cdc2e97751460a4658a999119645.pdfsafflowercallus inductionshoot regenerationexplanttissue culture |
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language |
fas |
format |
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DOAJ |
author |
javad Motamedi Alireza zebarjadi Danyal kahrizi Alihatef salmanian jaleh soheylikhah |
spellingShingle |
javad Motamedi Alireza zebarjadi Danyal kahrizi Alihatef salmanian jaleh soheylikhah Study of Callus Induction and Shoot Regeneration of Safflower (Carthamus tinctorius L.) Using Hypocotyl and Cotyledon Explants Culture مجله بیوتکنولوژی کشاورزی safflower callus induction shoot regeneration explant tissue culture |
author_facet |
javad Motamedi Alireza zebarjadi Danyal kahrizi Alihatef salmanian jaleh soheylikhah |
author_sort |
javad Motamedi |
title |
Study of Callus Induction and Shoot Regeneration of Safflower (Carthamus tinctorius L.) Using Hypocotyl and Cotyledon Explants Culture |
title_short |
Study of Callus Induction and Shoot Regeneration of Safflower (Carthamus tinctorius L.) Using Hypocotyl and Cotyledon Explants Culture |
title_full |
Study of Callus Induction and Shoot Regeneration of Safflower (Carthamus tinctorius L.) Using Hypocotyl and Cotyledon Explants Culture |
title_fullStr |
Study of Callus Induction and Shoot Regeneration of Safflower (Carthamus tinctorius L.) Using Hypocotyl and Cotyledon Explants Culture |
title_full_unstemmed |
Study of Callus Induction and Shoot Regeneration of Safflower (Carthamus tinctorius L.) Using Hypocotyl and Cotyledon Explants Culture |
title_sort |
study of callus induction and shoot regeneration of safflower (carthamus tinctorius l.) using hypocotyl and cotyledon explants culture |
publisher |
Shahid Bahonar University of Kerman |
series |
مجله بیوتکنولوژی کشاورزی |
issn |
2228-6705 2228-6500 |
publishDate |
2010-05-01 |
description |
After cereals, oilseed crops are included as the second source of supplying calorie for human populations. In recent years, safflower was considered due to quality and high content of fatty acids. For optimization of tissue culture of safflower, an experiment was carried out as a factorial arrangement in a completely randomized design with three replications in 2008-2009. Callus induction and shoot regeneration percentage of cotyledons and hypocotyl explants were measured on basal MS medium for two cultivars (Dincer and Sina) of safflower in different concentrations of NAA and BAP hormones. Analysis of interaction effects indicated that the cultivars were independent in response of different level of NAA. Mean comparison showed that the highest percentage of callus induction occurred on MS medium containing 0.5 mg/l NAA and 0.5 mg/l BAP (94.33%) and 1 mg/l NAA and 1 mg/l BAP (97%). Furthermore, hypocotyl explant of Dincer has a good response to callus induction. The highest percentage of shoot regeneration also was achieved on MS medium supplemented with 0.1 mg/l NAA and 2 mg/l BAP from cotyledon explant of Dincer. |
topic |
safflower callus induction shoot regeneration explant tissue culture |
url |
https://jab.uk.ac.ir/article_364_a962cdc2e97751460a4658a999119645.pdf |
work_keys_str_mv |
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