ESBL, MBL and Ampc β Lactamases Producing Superbugs – Havoc in the Intensive Care Units of Punjab India
ABSTRACT Background: An alarming rise in the rates of the antibiotic resistance has now become a serious and an increasingly common public health concern, with severe implications, especially in the intensive care units. A variety of ß-lactamases which include ESBLs, AmpC ß-lactamases and metal...
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doaj-67137971c38241fdb943a908b8c4bec62020-11-25T02:41:30ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2013-01-0171707310.7860/JCDR/2012/5016.2673ESBL, MBL and Ampc β Lactamases Producing Superbugs – Havoc in the Intensive Care Units of Punjab India Loveena Oberoi0 Nachhatarjit Singh1 Poonam Sharma2Aruna Aggarwal3Professor, Department of MicrobiologyPhD Student, Department of MicrobiologyAssistant Professor, Department of MicrobiologyProfessor & Head, Department of Microbiology, Sri Guru Ram Das Institute of Medical Sciences & Research,Vallah Amritsar Panjab, India.ABSTRACT Background: An alarming rise in the rates of the antibiotic resistance has now become a serious and an increasingly common public health concern, with severe implications, especially in the intensive care units. A variety of ß-lactamases which include ESBLs, AmpC ß-lactamases and metallo-ßlactamases, have emerged as the most worrisome mechanism of resistance among the gram negative bacteria, which pose a therapeutic challenge to the health care settings. Materials and Methods: The present study was aimed at knowing the prevalence of various ß-lactamases in the gram negative isolates which were obtained from ICU patients. A total 273 gram negative isolates from 913 clinical samples which were received over a period of one year were processed for their identification and their antimicrobial susceptibility pattern was determined. They were then screened for the ß-lactamase production. Results: Among the 273 isolates, the ß-lactamase production was observed in 193 strains. 96 (35.16%) strains were ESBL producers, followed by 30 (10.98%) metallo ß- lactamase (MBL) producers and 15(5.4%) AmpC producers. The major ESBL and AmpC producer was Escherichia coli, while Klebsiella pneumonia was the predominant MBL producer. The co production of the ESBL/MBL/ AmpC ß- lactamases was observed in 52 (19.04%) strains and it was more common in Escherichia coli. A multidrug resistance to the fluoroquinolones and the aminoglycosides was also observed in the ß- lactamase producing organisms. Conclusion: The high prevalence of the ß- lactamases in the ICU isolates emphasizes the need for a continuous surveillance in the ICUs to detect the resistant strains, strict guidelines for the antibiotic therapy and the implementation of infection control measures to reduce the increasing burden of antibiotic resistance.https://jcdr.net/articles/PDF/2673/18-%205016_E(C)_PF1(M)_F(P)_PFA(PM)_OLF_PF1(RP)_u.pdfextended spectrum ß-lactamases (esbls)ampc ß-lactamasesmetallo-ß-lactamases (mbls)antibiotic resistanceintensive care units (icus) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Loveena Oberoi Nachhatarjit Singh Poonam Sharma Aruna Aggarwal |
spellingShingle |
Loveena Oberoi Nachhatarjit Singh Poonam Sharma Aruna Aggarwal ESBL, MBL and Ampc β Lactamases Producing Superbugs – Havoc in the Intensive Care Units of Punjab India Journal of Clinical and Diagnostic Research extended spectrum ß-lactamases (esbls) ampc ß-lactamases metallo-ß-lactamases (mbls) antibiotic resistance intensive care units (icus) |
author_facet |
Loveena Oberoi Nachhatarjit Singh Poonam Sharma Aruna Aggarwal |
author_sort |
Loveena Oberoi |
title |
ESBL, MBL and Ampc β Lactamases Producing Superbugs – Havoc in the Intensive Care Units of Punjab India |
title_short |
ESBL, MBL and Ampc β Lactamases Producing Superbugs – Havoc in the Intensive Care Units of Punjab India |
title_full |
ESBL, MBL and Ampc β Lactamases Producing Superbugs – Havoc in the Intensive Care Units of Punjab India |
title_fullStr |
ESBL, MBL and Ampc β Lactamases Producing Superbugs – Havoc in the Intensive Care Units of Punjab India |
title_full_unstemmed |
ESBL, MBL and Ampc β Lactamases Producing Superbugs – Havoc in the Intensive Care Units of Punjab India |
title_sort |
esbl, mbl and ampc β lactamases producing superbugs – havoc in the intensive care units of punjab india |
publisher |
JCDR Research and Publications Private Limited |
series |
Journal of Clinical and Diagnostic Research |
issn |
2249-782X 0973-709X |
publishDate |
2013-01-01 |
description |
ABSTRACT
Background: An alarming rise in the rates of the antibiotic
resistance has now become a serious and an increasingly
common public health concern, with severe implications,
especially in the intensive care units. A variety of ß-lactamases
which include ESBLs, AmpC ß-lactamases and metallo-ßlactamases, have emerged as the most worrisome mechanism
of resistance among the gram negative bacteria, which pose a
therapeutic challenge to the health care settings.
Materials and Methods: The present study was aimed at
knowing the prevalence of various ß-lactamases in the gram
negative isolates which were obtained from ICU patients. A total
273 gram negative isolates from 913 clinical samples which
were received over a period of one year were processed for
their identification and their antimicrobial susceptibility pattern
was determined. They were then screened for the ß-lactamase
production.
Results: Among the 273 isolates, the ß-lactamase production
was observed in 193 strains. 96 (35.16%) strains were ESBL
producers, followed by 30 (10.98%) metallo ß- lactamase (MBL)
producers and 15(5.4%) AmpC producers. The major ESBL and
AmpC producer was Escherichia coli, while Klebsiella pneumonia
was the predominant MBL producer. The co production of the
ESBL/MBL/ AmpC ß- lactamases was observed in 52 (19.04%)
strains and it was more common in Escherichia coli. A multidrug
resistance to the fluoroquinolones and the aminoglycosides was
also observed in the ß- lactamase producing organisms.
Conclusion: The high prevalence of the ß- lactamases in the
ICU isolates emphasizes the need for a continuous surveillance
in the ICUs to detect the resistant strains, strict guidelines
for the antibiotic therapy and the implementation of infection
control measures to reduce the increasing burden of antibiotic
resistance. |
topic |
extended spectrum ß-lactamases (esbls) ampc ß-lactamases metallo-ß-lactamases (mbls) antibiotic resistance intensive care units (icus) |
url |
https://jcdr.net/articles/PDF/2673/18-%205016_E(C)_PF1(M)_F(P)_PFA(PM)_OLF_PF1(RP)_u.pdf |
work_keys_str_mv |
AT loveenaoberoi esblmblandampcblactamasesproducingsuperbugshavocintheintensivecareunitsofpunjabindia AT nachhatarjitsingh esblmblandampcblactamasesproducingsuperbugshavocintheintensivecareunitsofpunjabindia AT poonamsharma esblmblandampcblactamasesproducingsuperbugshavocintheintensivecareunitsofpunjabindia AT arunaaggarwal esblmblandampcblactamasesproducingsuperbugshavocintheintensivecareunitsofpunjabindia |
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