Diversity of A(H5N1) clade 2.3.2.1c avian influenza viruses with evidence of reassortment in Cambodia, 2014-2016.
In Cambodia, highly pathogenic avian influenza A(H5N1) subtype viruses circulate endemically causing poultry outbreaks and zoonotic human cases. To investigate the genomic diversity and development of endemicity of the predominantly circulating clade 2.3.2.1c A(H5N1) viruses, we characterised 68 AIV...
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doaj-6728153933ca4a05beeabe92aca666d72021-03-03T21:18:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-011412e022610810.1371/journal.pone.0226108Diversity of A(H5N1) clade 2.3.2.1c avian influenza viruses with evidence of reassortment in Cambodia, 2014-2016.Annika SuttieSongha TokSokhoun YannPonnarath KeoSrey Viseth HormMerryn RoeMatthew KayeSan SornDavun HollSothyra TumPhilippe BuchyIan BarrAeron HurtAndrew R GreenhillErik A KarlssonDhanasekaran VijaykrishnaYi-Mo DengPhilippe DussartPaul F HorwoodIn Cambodia, highly pathogenic avian influenza A(H5N1) subtype viruses circulate endemically causing poultry outbreaks and zoonotic human cases. To investigate the genomic diversity and development of endemicity of the predominantly circulating clade 2.3.2.1c A(H5N1) viruses, we characterised 68 AIVs detected in poultry, the environment and from a single human A(H5N1) case from January 2014 to December 2016. Full genomes were generated for 42 A(H5N1) viruses. Phylogenetic analysis shows that five clade 2.3.2.1c genotypes, designated KH1 to KH5, were circulating in Cambodia during this period. The genotypes arose through multiple reassortment events with the neuraminidase (NA) and internal genes belonging to H5N1 clade 2.3.2.1a, clade 2.3.2.1b or A(H9N2) lineages. Phylogenies suggest that the Cambodian AIVs were derived from viruses circulating between Cambodian and Vietnamese poultry. Molecular analyses show that these viruses contained the hemagglutinin (HA) gene substitutions D94N, S133A, S155N, T156A, T188I and K189R known to increase binding to the human-type α2,6-linked sialic acid receptors. Two A(H5N1) viruses displayed the M2 gene S31N or A30T substitutions indicative of adamantane resistance, however, susceptibility testing towards neuraminidase inhibitors (oseltamivir, zanamivir, lananmivir and peramivir) of a subset of thirty clade 2.3.2.1c viruses showed susceptibility to all four drugs. This study shows that A(H5N1) viruses continue to reassort with other A(H5N1) and A(H9N2) viruses that are endemic in the region, highlighting the risk of introduction and emergence of novel A(H5N1) genotypes in Cambodia.https://doi.org/10.1371/journal.pone.0226108 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Annika Suttie Songha Tok Sokhoun Yann Ponnarath Keo Srey Viseth Horm Merryn Roe Matthew Kaye San Sorn Davun Holl Sothyra Tum Philippe Buchy Ian Barr Aeron Hurt Andrew R Greenhill Erik A Karlsson Dhanasekaran Vijaykrishna Yi-Mo Deng Philippe Dussart Paul F Horwood |
spellingShingle |
Annika Suttie Songha Tok Sokhoun Yann Ponnarath Keo Srey Viseth Horm Merryn Roe Matthew Kaye San Sorn Davun Holl Sothyra Tum Philippe Buchy Ian Barr Aeron Hurt Andrew R Greenhill Erik A Karlsson Dhanasekaran Vijaykrishna Yi-Mo Deng Philippe Dussart Paul F Horwood Diversity of A(H5N1) clade 2.3.2.1c avian influenza viruses with evidence of reassortment in Cambodia, 2014-2016. PLoS ONE |
author_facet |
Annika Suttie Songha Tok Sokhoun Yann Ponnarath Keo Srey Viseth Horm Merryn Roe Matthew Kaye San Sorn Davun Holl Sothyra Tum Philippe Buchy Ian Barr Aeron Hurt Andrew R Greenhill Erik A Karlsson Dhanasekaran Vijaykrishna Yi-Mo Deng Philippe Dussart Paul F Horwood |
author_sort |
Annika Suttie |
title |
Diversity of A(H5N1) clade 2.3.2.1c avian influenza viruses with evidence of reassortment in Cambodia, 2014-2016. |
title_short |
Diversity of A(H5N1) clade 2.3.2.1c avian influenza viruses with evidence of reassortment in Cambodia, 2014-2016. |
title_full |
Diversity of A(H5N1) clade 2.3.2.1c avian influenza viruses with evidence of reassortment in Cambodia, 2014-2016. |
title_fullStr |
Diversity of A(H5N1) clade 2.3.2.1c avian influenza viruses with evidence of reassortment in Cambodia, 2014-2016. |
title_full_unstemmed |
Diversity of A(H5N1) clade 2.3.2.1c avian influenza viruses with evidence of reassortment in Cambodia, 2014-2016. |
title_sort |
diversity of a(h5n1) clade 2.3.2.1c avian influenza viruses with evidence of reassortment in cambodia, 2014-2016. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2019-01-01 |
description |
In Cambodia, highly pathogenic avian influenza A(H5N1) subtype viruses circulate endemically causing poultry outbreaks and zoonotic human cases. To investigate the genomic diversity and development of endemicity of the predominantly circulating clade 2.3.2.1c A(H5N1) viruses, we characterised 68 AIVs detected in poultry, the environment and from a single human A(H5N1) case from January 2014 to December 2016. Full genomes were generated for 42 A(H5N1) viruses. Phylogenetic analysis shows that five clade 2.3.2.1c genotypes, designated KH1 to KH5, were circulating in Cambodia during this period. The genotypes arose through multiple reassortment events with the neuraminidase (NA) and internal genes belonging to H5N1 clade 2.3.2.1a, clade 2.3.2.1b or A(H9N2) lineages. Phylogenies suggest that the Cambodian AIVs were derived from viruses circulating between Cambodian and Vietnamese poultry. Molecular analyses show that these viruses contained the hemagglutinin (HA) gene substitutions D94N, S133A, S155N, T156A, T188I and K189R known to increase binding to the human-type α2,6-linked sialic acid receptors. Two A(H5N1) viruses displayed the M2 gene S31N or A30T substitutions indicative of adamantane resistance, however, susceptibility testing towards neuraminidase inhibitors (oseltamivir, zanamivir, lananmivir and peramivir) of a subset of thirty clade 2.3.2.1c viruses showed susceptibility to all four drugs. This study shows that A(H5N1) viruses continue to reassort with other A(H5N1) and A(H9N2) viruses that are endemic in the region, highlighting the risk of introduction and emergence of novel A(H5N1) genotypes in Cambodia. |
url |
https://doi.org/10.1371/journal.pone.0226108 |
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