Efficient ultrafiltration-based protocol to deplete extracellular vesicles from fetal bovine serum

Efficient ultrafiltration-based protocol to deplete extracellular vesicles from fetal bovine serum

Fetal bovine serum (FBS) is the most commonly used supplement in studies involving cell-culture experiments. However, FBS contains large numbers of bovine extracellular vesicles (EVs), which hamper the analyses of secreted EVs from the cell type of preference and, thus, also the downstream analyses....

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Bibliographic Details
Main Authors: Roman Kornilov, Maija Puhka, Bettina Mannerström, Hanna Hiidenmaa, Hilkka Peltoniemi, Pia Siljander, Riitta Seppänen-Kaijansinkko, Sippy Kaur
Format: Article
Language:English
Published: Taylor & Francis Group 2018-12-01
Series:Journal of Extracellular Vesicles
Subjects:
adipose-tissue mesenchymal stem cells
extracellular vesicles
fetal bovine serum
depleted serum
ultracentrifugation
ultrafiltration
Online Access:http://dx.doi.org/10.1080/20013078.2017.1422674

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